1887

Abstract

Three bacterial strains, designated DS48-6-5, DS48-6-7 and DS48-6-9, were isolated from a sediment sample taken from Daechung Reservoir (Republic of Korea) at a water depth of 48 m. Cells of the strains were Gram-stain-negative, aerobic, rod-shaped and motile with a single polar flagellum. Comparative 16S rRNA gene sequence studies showed that the three isolates had clear affiliation with and the closest relatives were KCTC 32299, DSM 11587 and KCTC 12591 with 97.2–97.9 % 16S rRNA gene sequence similarities; the 16S rRNA gene sequence similarities between the three strains were 99.5–100 %. The only isoprenoid quinone of the three strains was ubiquinone-8, and the major fatty acids were summed feature 3 (Cω6 and/or Cω7) and C. The G+C content of the genomic DNA of strains DS48-6-5, DS48-6-7 and DS48-6-9 was 66.7, 67.0 and 66.8 mol%, respectively. DNA–DNA hybridization values of the novel strains with KCTC 32299, DSM 11587 and KCTC 12591 were 19.3–48.5 %. Based on the evidence from this taxonomic study using a polyphasic approach, it is proposed that strains, DS48-6-5, DS48-6-7 and DS48-6-9, represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS48-6-5 ( = KCTC 42645 = NBRC 111169).

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2016-05-01
2019-10-15
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