1887

Abstract

A Gram-stain-positive, aerobic and non-motile actinobacterial strain, designated YIM C0533, was isolated from a soil sample collected from Shiling county, Yunnan province, south-west China. The isolate grew at 15–37 °C, pH 6.0–8.0 and in the presence of 0–3 % (w/v) NaCl. The whole-cell hydrolysates contained -diaminopimelic acid, xylose, galactose, mannose, ribose, arabinose, glucose and rhamnose. The acyl type of muramic acid was glycolyl. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified phospholipid. The major cellular fatty acids were iso-C, 10-methyl C and iso-C.MK-9(H) was the predominant menaquinone. The genomic DNA G+C content was determined to be 69.4 mol%. These chemotaxonomic data and the morphological properties were consistent with those of the genus . The strain showed highest sequence similarities to CGMCC 4.1403 on phylogenetic analysis of 16S rRNA gene sequences and was found to form a coherent cluster in the neighbour–joining tree. The DNA–DNA hybridization experiment indicated that the DNA–DNA relatedness value between strain YIM C0533 and CGMCC 4.1403 was 34.4 ± 1.3 %. In addition, the results of physiological and biochemical tests allowed the isolate to be differentiated phenotypically from CGMCC 4.1403. On the basis of data from this polyphasic study, strain YIM C0533 is characterized as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM C0533 ( = CPCC 204160 = KCTC 39591 = CGMCC 4.7289). An emended description of the genus is also provided.

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2016-04-01
2020-01-22
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