1887

Abstract

A Gram-reaction-negative, aerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated YX-36, was isolated from a vegetable plot in Yixing, Jiangsu province, China. The strain grew at 15–37 °C (optimally at 37 °C), at pH 6.0–9.5 (optimally at pH 6.5) and in the presence of 0–1 % (w/v) NaCl (optimally without NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YX-36 was related most closely to DR-9 (96.88 % similarity), followed by SMS-12 (95.78 %), DR-f3 (95.77 %) and DRP28 (95.77 %). The DNA G+C content of strain YX-36 was 47.2 mol%. The only isoprenoid quinone was menaquinone 7 (MK-7). The major polar lipids were phosphatidylethanolamine and aminophospholipid. The major fatty acids were iso-C, summed feature 3 (iso-C 2-OH/Cω7) and iso-C 3-OH. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain YX-36 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YX-36 ( = DSM 26809 = CCTCC AB 2012880).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000941
2016-04-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/4/1779.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000941&mimeType=html&fmt=ahah

References

  1. An D. S., Yin C. R., Lee S. T., Cho C. H.. 2009; Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. Int J Syst Evol Microbiol59:1122–1125 [CrossRef][PubMed]
    [Google Scholar]
  2. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N.. 2010; Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol60:134–139 [CrossRef][PubMed]
    [Google Scholar]
  3. Barrow G. I., Feltham R. K. A..editors 1993; Cowan and Steel's Manual for the Identification of Medical Bacteria New York: Cambridge University Press; [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol52:1049–1070[PubMed]
    [Google Scholar]
  5. Bowman J. P.. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L.. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  9. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  10. Joung Y., Joh K.. 2011; Mucilaginibacter myungsuensis sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol61:1506–1510 [CrossRef][PubMed]
    [Google Scholar]
  11. Kang S. J., Jung Y. T., Oh K. H., Oh T. K., Yoon J. H.. 2011; Mucilaginibacter boryungensis sp. nov., isolated from soil. Int J Syst Evol Microbiol61:1549–1553 [CrossRef][PubMed]
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  13. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  14. Lányí B.. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol19:1–67 [CrossRef]
    [Google Scholar]
  15. Lee H. R., Han S. I., Rhee K. H., Whang K. S.. 2013; Mucilaginibacter herbaticus sp. nov., isolated from the rhizosphere of the medicinal plant Angelica sinensis. Int J Syst Evol Microbiol63:2787–2793 [CrossRef][PubMed]
    [Google Scholar]
  16. Leifson E.. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol85:1183–1184[PubMed]
    [Google Scholar]
  17. MacFaddin J. F.. 1980; Biochemical Tests for Identification of Medical Bacteria, 2nd edn Baltimore: Williams & Wilkins;
    [Google Scholar]
  18. Madhaiyan M., Poonguzhali S., Lee J. S., Senthilkumar M., Lee K. C., Sundaram S.. 2010; Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. Int J Syst Evol Microbiol60:2451–2457 [CrossRef][PubMed]
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  20. Murray R. G. E., Doetsch R. N., Robinow F.. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp21–41Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  21. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N.. 2007; Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. Int J Syst Evol Microbiol57:2349–2354 [CrossRef][PubMed]
    [Google Scholar]
  22. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  23. Sambrook J., Russell D. W.. 2001; Molecular Cloning: a Laboratory Manual, 3rd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp607–654Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Tamura T., Hatano K.. 2001; Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol51:2119–2125 [CrossRef][PubMed]
    [Google Scholar]
  26. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  28. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M.. 2008; Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. Int J Syst Evol Microbiol58:2046–2050 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000941
Loading
/content/journal/ijsem/10.1099/ijsem.0.000941
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error