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Abstract

A Gram-stain-negative, non-motile, non-spore-forming, non-pigmented, oxidase- and catalase-positive bacterial strain, designated BIc20019, was isolated from the ice core of Austre Lovénbreen in Ny-Ålesund, Svalbard. The temperature and NaCl ranges for growth were 4–34 °C (optimum, 25–29 °C) and 0–8 % (w/v) (optimum, 2–4 %). Analysis of the 16S rRNA gene sequence indicated that strain BIc20019 belonged to the genus and was closely related to 273-4, K5, ‘’ BSw21516B, LMG 21280, LMG 21276 and MD17 at greater than 99 % similarity. Phylogenetic analysis based on gene sequences revealed highest similarity (93.6 %) to MD17. However, DNA hybridization experiments revealed a low level of DNA–DNA relatedness ( < 59 %) between strain BIc20019 and its closest relatives. Strain BIc20019 contained ubiquinone-8 (Q-8) as the predominant respiratory quinone, and Cω9 and summed feature 3 (Cω7 and/or iso-C 2-OH) as the major fatty acids. It had a DNA G+C content of 46.3 mol%. The polar lipid profile of strain BIc20019 was mainly composed of phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Owing to the differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene and gene sequences, and DNA–DNA relatedness data, the isolate merits classification within a novel species for which the name sp. nov. is proposed. The type strain is BIc20019 ( = KCTC 42280 = CCTCC AB 2014019).

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2016-04-01
2022-01-29
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