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Abstract

A Gram-stain-negative, non-motile, non-spore-forming, non-pigmented, oxidase- and catalase-positive bacterial strain, designated BIc20019, was isolated from the ice core of Austre Lovénbreen in Ny-Ålesund, Svalbard. The temperature and NaCl ranges for growth were 4–34 °C (optimum, 25–29 °C) and 0–8 % (w/v) (optimum, 2–4 %). Analysis of the 16S rRNA gene sequence indicated that strain BIc20019 belonged to the genus Psychrobacter and was closely related to Psychrobacter arcticus 273-4, Psychrobacter cryohalolentis K5, ‘Psychrobacter fjordensis’ BSw21516B, Psychrobacter fozii LMG 21280, Psychrobacter luti LMG 21276 and Pyschrobacter okhotskensis MD17 at greater than 99 % similarity. Phylogenetic analysis based on gyrB gene sequences revealed highest similarity (93.6 %) to P. okhotskensis MD17. However, DNA hybridization experiments revealed a low level of DNA–DNA relatedness ( < 59 %) between strain BIc20019 and its closest relatives. Strain BIc20019 contained ubiquinone-8 (Q-8) as the predominant respiratory quinone, and C18 : 1ω9c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) as the major fatty acids. It had a DNA G+C content of 46.3 mol%. The polar lipid profile of strain BIc20019 was mainly composed of phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Owing to the differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene and gyrB gene sequences, and DNA–DNA relatedness data, the isolate merits classification within a novel species for which the name Psychrobacter glaciei sp. nov. is proposed. The type strain is BIc20019 ( = KCTC 42280 = CCTCC AB 2014019).

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2016-04-01
2019-10-22
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References

  1. Bakermans C., Ayala-del-Río H. L., Ponder M. A., Vishnivetskaya T., Gilichinsky D., Thomashow M. F., Tiedje J. M.. ( 2006;). Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost. Int J Syst Evol Microbiol 56: 1285–1291 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bowers R. M., Lauber C. L., Wiedinmyer C., Hamady M., Hallar A. G., Fall R., Knight R., Fierer N.. ( 2009;). Characterization of airborne microbial communities at a high-elevation site and their potential to act as atmospheric ice nuclei. Appl Environ Microbiol 75: 5121–5130 [CrossRef] [PubMed].
    [Google Scholar]
  3. Bowman J. P., Cavanagh J., Austin J. J., Sanderson K.. ( 1996;). Novel Psychrobacter species from Antarctic ornithogenic soils. Int J Syst Bacteriol 46: 841–848 [CrossRef] [PubMed].
    [Google Scholar]
  4. Bozal N., Montes M. J., Tudela E., Guinea J.. ( 2003;). Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp. nov. and Psychrobacter fozii sp. nov. Int J Syst Evol Microbiol 53: 1093–1100 [CrossRef] [PubMed].
    [Google Scholar]
  5. Fahlgren C., Hagström A., Nilsson D., Zweifel U. L.. ( 2010;). Annual variations in the diversity, viability, and origin of airborne bacteria. Appl Environ Microbiol 76: 3015–3025 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  7. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  8. Huss V. A., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4: 184–192 [CrossRef] [PubMed].
    [Google Scholar]
  9. Juni E., Heym G. A.. ( 1986;). Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36: 388–391 [CrossRef].
    [Google Scholar]
  10. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  11. Lee Y. K., Sung K. C., Yim J. H., Park K. J., Chung H., Lee H. K.. ( 2005;). Isolation of protease-producing Arctic marine bacteria. Ocean Polar Res 27: 215–219 [CrossRef].
    [Google Scholar]
  12. Maruyama A., Honda D., Yamamoto H., Kitamura K., Higashihara T.. ( 2000;). Phylogenetic analysis of psychrophilic bacteria isolated from the Japan Trench, including a description of the deep-sea species Psychrobacter pacificensis sp. nov. Int J Syst Evol Microbiol 50: 835–846 [CrossRef] [PubMed].
    [Google Scholar]
  13. MIDI ( 1999;). Sherlock Microbial Identification System Operation Manual, version 3.0 Newark, DE: MIDI Inc;.
    [Google Scholar]
  14. Rodrigues D. F., da C Jesus E., Ayala-Del-Río H. L., Pellizari V. H., Gilichinsky D., Sepulveda-Torres L., Tiedje J. M.. ( 2009;). Biogeography of two cold-adapted genera: Psychrobacter and Exiguobacterium. ISME J 3: 658–665 [CrossRef] [PubMed].
    [Google Scholar]
  15. Romanenko L. A., Lysenko A. M., Rohde M., Mikhailov V. V., Stackebrandt E.. ( 2004;). Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan. Int J Syst Evol Microbiol 54: 1741–1745 [CrossRef] [PubMed].
    [Google Scholar]
  16. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  17. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V.. ( 1986;). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5: 139–156 [CrossRef].
    [Google Scholar]
  18. Srinivas T. N. R., Singh S. M., Pradhan S., Pratibha M. S., Kishore K. H., Singh A. K., Begum Z., Prabagaran S. R., Reddy G. S. N., Shivaji S.. ( 2011;). Comparison of bacterial diversity in proglacial soil from Kafni Glacier, Himalayan Mountain ranges, India, with the bacterial diversity of other glaciers in the world. Extremophiles 15: 673–690 [CrossRef] [PubMed].
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  21. Tindall B. J.. ( 1990;). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  23. Xie C. H., Yokota A.. ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49: 345–349 [CrossRef] [PubMed].
    [Google Scholar]
  24. Yumoto I., Hirota K., Sogabe Y., Nodasaka Y., Yokota Y., Hoshino T.. ( 2003;). Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea. Int J Syst Evol Microbiol 53: 1985–1989 [CrossRef] [PubMed].
    [Google Scholar]
  25. Zeng Y., Liu W., Li H., Yu Y., Chen B.. ( 2007;). Effect of restriction endonucleases on assessment of biodiversity of cultivable polar marine planktonic bacteria by amplified ribosomal DNA restriction analysis. Extremophiles 11: 685–692 [CrossRef] [PubMed].
    [Google Scholar]
  26. Zeng Y., Zheng T., Yu Y., Chen B., He J.. ( 2010;). Relationships between Arctic and Antarctic Shewanella strains evaluated by a polyphasic taxonomic approach. Polar Biol 33: 531–541 [CrossRef].
    [Google Scholar]
  27. Zeng Y. X., Yu Y., Li H. R., Luo W.. ( 2015;). Psychrobacter fjordensis sp. nov., a psychrotolerant bacterium isolated from an Arctic fjord in Svalbard. Antonie van Leeuwenhoek 108: 1283–1292 [CrossRef] [PubMed].
    [Google Scholar]
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