1887

Abstract

A survey to obtain potential antagonists of pome fruit tree diseases yielded two yellow epiphytic bacterial isolates morphologically similar to , but showing no biocontrol activity. Whole-cell MALDI-TOF mass spectrometry and analysis of 16S rRNA gene and sequences suggested the possibility of a novel species with a phylogenetic position in either the genus or the genus . Multi-locus sequence analysis (MLSA) placed the two strains in the genus and supported their classification as a novel species. The strains showed general phenotypic characteristics typical of this genus and results of DNA–DNA hybridizations confirmed that they represent a single novel species. Both strains showed a DNA G+C content, as determined by HPLC, of 54.5 mol% and could be discriminated from phylogenetically related species of the genus by their ability to utilize potassium gluconate, potassium 2-ketogluconate, maltose, melibiose and raffinose. Whole-genome sequencing of strain EM595 revealed the presence of a chromosomal carotenoid biosynthesis gene cluster similar to those found in species of the genera and that explains the pigmentation of the strain, which is atypical for the genus . Additional strains belonging to the same species were recovered from different plant hosts in three different continents, revealing the cosmopolitan nature of this epiphyte. The name sp. nov. is proposed, with EM595 ( = LMG 28990 = CCOS 903) as the designated type strain.

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2016-03-01
2019-12-15
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