1887

Abstract

A Gram-stain-positive, non-endospore-forming, haloalkaliphilic actinobacterium, strain CK5, was isolated from a soil sample, collected at Cape King (Antarctica), and its taxonomic position was investigated by using a polyphasic approach. Cells were cocci with orange pigmentation, non-motile and grew optimally at 25 °C and pH 9.0–9.5 in the presence of 2 % (w/v) NaCl. Cellular membrane contained MK-7 (72 %) and MK-8 (28 %), and anteiso-C (64.8 %), iso-C (13.3 %), -C (9.9 %), -C (4.0 %), -C (3.7 %) as major cellular fatty acids. The DNA G+C content was 64.8 mol%. Strain CK5, based on the 16S rRNA gene sequence similarity, was most closely related to JG-241 (99.5 %), YIM 70009 (99.4 %), YIM 70081 (99.4 %), YIM 70084 (99.3 %), YIM 70097 (97.2 %), CAAS 251 (97.1 %) and CCUG 48939 (97.1 %). Strain CK5 revealed 31 % DNA–DNA relatedness with respect to DSM 15664, 29 % with respect to DSM 19081, 10 % with respect to DSM 15666 and 1 % with respect to DSM 15474, DSM 15475, DSM 17733 and DSM 19422. On the basis of 16S rRNA gene sequences, DNA–DNA hybridization and chemotaxonomic characteristics, strain CK5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CK5 ( = DSM 27373 = JCM 19723).

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2016-03-01
2019-09-22
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