1887

Abstract

Species of the genus are achlorophyllous algae and ubiquitous in nature, and so far, six species have been listed in this genus: , , , , and . A strain of the genus , IFM 53848, was isolated in Japan from a patient with systemic protothecosis and had been designated . Our previous study, by using PCR analysis, revealed that its SSU rRNA gene (rDNA) was distinctively larger than that of and other species of the genus . In this study, molecular analysis showed that the exceptionally large SSU rDNA of IFM 53848 contains four group I introns. The morphology of IFM 53848 was indistinguishable from those of or , and phylogenetic analyses, based on the sequences of the SSU rDNA exons and the D1/D2 region of the large subunit rDNA, indicated that IFM 53848 was closely related to . On the other hand, unlike , IFM 53848 failed to assimilate fructose or lysine and grew well at higher temperatures of up to 42 °C. In addition, the nucleotide sequence of the ribosomal internal transcribed spacer and the matrix assisted laser desorption ionization time-of-flight mass spectrometry profile of IFM 53848 were clearly distinct from those of . The results strongly suggest that IFM 53848 represents a novel species, and so the seventh member of the genus , which we have named sp. nov. The unique characteristics of the strain may provide useful insights into the systematic taxonomy of the genus .

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2016-03-01
2020-01-18
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