1887

Abstract

A novel actinobacterium, designated strain SG20, was isolated from a Saharan soil sample collected from Béni-isguen (Mzab), Ghardaïa province, southern Algeria. The micro-organism developed small roundish sporangia on aerial mycelium that were sessile or carried by very short sporangiophores. The cell-wall peptidoglycan contained -diaminopimelic acid and the whole-cell sugars comprised glucose, ribose and mannose, but madurose was not detected. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were iso-C and C. The phospholipids detected were diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and unknown lipids. The phenotypic and chemotaxonomic characteristics of the novel strain resembled those of recognized members of the genus . Moreover, phylogenetic analysis based on a 16S rRNA gene sequence generated from the strain identified its closest relative as BCC 53154 (98.5 % similarity), which produces single spores on aerial mycelium, but no sporangia. In hybridization experiments, the DNA–DNA relatedness values recorded between strain SG20 and DSM 46822 fell well below 70 %. On the basis of phenotypic and genotypic data, strain SG20 can be distinguished as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SG20 ( = DSM 46743 = CECT 8840).

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2016-03-01
2022-01-18
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