1887

Abstract

A Gram-stain positive, endospore-forming, strictly anaerobic bacterium, designated strain Gal1, was isolated from shea cake, a waste material from the production of shea butter, originating from Saraya, Senegal. The cells were rod-shaped, slightly curved, and motile with peritrichous flagella. The strain was oxidase-negative and catalase-negative. Growth was observed at temperatures ranging from 15 to 45 °C (optimum 30 °C) and at pH 6.5–9.3 (optimum pH 7.8). The salinity range for growth was 0–3.5 % NaCl (optimum 1 %). Yeast extract was required for growth. Strain Gal1 fermented various carbohydrates such as mannose, mannitol, arabinose, cellobiose, fructose, glucose, maltose, sucrose, trehalose and lactose and the major end-products were ethanol and acetate. The only major cellular fatty acid was C16 : 0 (19.6 %). The DNA base G+C content of strain Gal1 was 33.8 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that this strain was related to DSM 26468 with 94.27 % similarity, ATTC 35295 with 93.94 % similarity, and DSM 1283 and DSM 15930 with 93.63 % similarity. On the basis of phenotypic characteristics, phylogenetic analysis and the results of biochemical and physiological tests, strain Gal1 was clearly distinguished from closely related genera, and strain Gal1 can be assigned to a novel species of a new genus for which the name gen. nov., sp. nov. is proposed. The type strain is Gal1 ( = DSM 26537 = JCM 18753).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000889
2016-03-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/3/1383.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000889&mimeType=html&fmt=ahah

References

  1. Benson D. A., Boguski M. S., Lipman D. J., Ostell J., Ouellette B. F. F., Rapp B. A., Wheeler D. L.. 1999; GenBank. Nucleic Acids Res27:12–17 [CrossRef][PubMed]
    [Google Scholar]
  2. Bhatta R., Mani S., Baruah L., Sampath K. T.. 2012; Phenolic composition, fermentation profile, protozoa population and methane production from Sheanut (Butyrospermum parkii) byproducts in vitro. Asian-Australas J Anim Sci25:1389–1394[CrossRef]
    [Google Scholar]
  3. Edgar R. C.. 2004; muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res32:1792–1797 [CrossRef][PubMed]
    [Google Scholar]
  4. Hall T. A.. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis proGram for Windows 95/98/NT. Nucleic Acids Symp Ser41:95–98
    [Google Scholar]
  5. Hardman J. K., Stadtman T. C.. 1960; Metabolism of ω-acids. II. Fermentation of delta-aminovaleric acid by Clostridium aminovalericum n. sp. J Bacteriol79:549–552[PubMed]
    [Google Scholar]
  6. Hungate R. E.. 1969; A roll tube method for the cultivation of strict anaerobes. Methods Microbiol 3B117–132 [CrossRef]
    [Google Scholar]
  7. Jeong H., Lim Y. W., Yi H., Sekiguchi Y., Kamagata Y., Chun J.. 2007; Anaerosporobacter mobilis gen. nov., sp. nov., isolated from forest soil. Int J Syst Evol Microbiol57:1784–1787 [CrossRef][PubMed]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R.. 1969; Evolution of protein molecules. In Mammalian Protein Metabolismvol. 3 pp21–132Edited by Munro H. N.. 3 New York: Academic press; [CrossRef]
    [Google Scholar]
  9. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E.. 1988; Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol38:358–361 [CrossRef]
    [Google Scholar]
  10. Macy J. M., Snellen J. E., Hungate R. E.. 1972; Use of syringe methods for anaerobiosis. Am J Clin Nutr25:1318–1323[PubMed]
    [Google Scholar]
  11. Maidak B. L., Cole J. R., Lilburn T. G., Parker C. T. Jr, Saxman P. R., Farris R. J., Garrity G. M., Olsen G. J., Schmidt T. M., Tiedje J. M.. 2001; The RDP-II (Ribosomal Database Project). Nucleic Acids Res29:173–174 [CrossRef][PubMed]
    [Google Scholar]
  12. Mechichi T., Labat M., Garcia J. L., Thomas P., Patel B. K. C.. 1999; Sporobacterium olearium gen. nov. sp. nov., a new methanethiol-producing bacterium that degrades aromatic compounds, isolated from an olive mill wastewater treatment digester. Int J Syst Bacteriol49:1741–1748 [CrossRef][PubMed]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  14. Miller L. T.. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol16:584–586[PubMed]
    [Google Scholar]
  15. Miller T. L., Wolin M. J.. 1974; A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol27:985–987[PubMed]
    [Google Scholar]
  16. Podosokorskaya O. A., Bonch-Osmolovskaya E. A., Beskorovaynyy A. V., Toshchakov S. V., Kolganova T. V., Kublanov I. V.. 2014; Mobilitalea sibirica gen. nov., sp. nov., a halotolerant polysaccharide-degrading bacterium. Int J Syst Evol Microbiol64:2657–2661 [CrossRef][PubMed]
    [Google Scholar]
  17. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  18. Sasser M.. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl20:16
    [Google Scholar]
  19. Sleat R., Mah R. A.. 1985; Clostridium populeti sp. nov., a cellulolytic species from a woody-biomass digestor. Int J Syst Bacteriol35:160–163 [CrossRef]
    [Google Scholar]
  20. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  21. Widdel F., Pfennig N.. 1981; Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from saline environments. Description of Desulfobacter postgatei gen. nov., sp. nov. Arch Microbiol129:395–400 [CrossRef][PubMed]
    [Google Scholar]
  22. S., Lebeau F., Wathelet J. P., Leemans V., Destain M. F.. 2007; Étude des paramètres opératoires de pressage mécanique des amandes de Vitellaria paradoxa Gaertn C.F. (karité). Biotechnol Agron Soc Environ11:267–273
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000889
Loading
/content/journal/ijsem/10.1099/ijsem.0.000889
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error