1887

Abstract

A novel strain of actinobacteria, designated strain NEAU-ZJC8, was isolated from a saline–alkaline soil collected from Heilongjiang Province, north-east China and characterized using a polyphasic approach. Strain NEAU-ZJC8 exhibited morphological, cultural and chemotaxonomic features consistent with its classification as representing a member of the genus . Growth occurred at 16–35 °C, pH 5.0–11.0 and in the presence of 0–11 % (w/v) NaCl. The cell-wall peptidoglycan contained -diaminopimelic acid and glycine. Whole-cell hydrolysates mainly contained glucose, galactose and ribose. Predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unidentified phospholipids. The major cellular fatty acids (>10 %) were iso-C, iso-C, anteiso-C and C. The G+C content of the DNA was 72.7 mol%. A phylogenetic tree based on 16S rRNA sequences showed that strain NEAU-ZJC8 formed a distinct clade within the genus and was closely related to DSM 41970 (98.56 % similarity) and NBRC 105047 (97.96 % similarity). A combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-ZJC8 could be distinguished from its closest phylogenetic relatives. Therefore, strain NEAU-ZJC8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-ZJC8 ( = CGMCC 4.7178 = JCM 30057).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000887
2016-03-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/3/1358.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000887&mimeType=html&fmt=ahah

References

  1. Anderson A. S., Wellington E. M. H. 2001; The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51:797–814 [View Article][PubMed]
    [Google Scholar]
  2. Antony-Babu S., Goodfellow M. 2008; Biosystematics of alkaliphilic streptomycetes isolated from seven locations across a beach and dune sand system. Antonie van Leeuwenhoek 94:581–591 [View Article][PubMed]
    [Google Scholar]
  3. Antony-Babu S., Stach J. E. M., Goodfellow M. 2010; Computer-assisted numerical analysis of colour-group data for dereplication of streptomycetes for bioprospecting and ecological purposes. Antonie van Leeuwenhoek 97:231–239 [View Article][PubMed]
    [Google Scholar]
  4. Chaudhary H. S., Soni B., Shrivastava A. R., Shrivastava S. 2013; Diversity and versatility of actinomycetes and its role in antibiotic production. J Appl Pharma Sci 3:S83–S94 [View Article]
    [Google Scholar]
  5. Collins M. D. 1985; Isoprenoid quinone analyses in bacterial classification and identification. In Chemical Methods in Bacterial Systematics pp 267–284Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  7. Duan Y.-Y., Ming H., Dong L., Yin Y.-R., Zhang Y., Zhou E.-M., Liu L., Nie G.-X., Li W.-J. 2014; Streptomyces calidiresistens sp. nov., isolated from a hot spring sediment. Antonie van Leeuwenhoek 106:189–196 [View Article][PubMed]
    [Google Scholar]
  8. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789 [View Article]
    [Google Scholar]
  10. Goodfellow M., Fiedler H. P. 2010; A guide to successful bioprospecting: informed by actinobacterial systematics. Antonie van Leeuwenhoek 98:119–142 [View Article][PubMed]
    [Google Scholar]
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
    [Google Scholar]
  12. Hamedi J., Mohammadipanah F., Ventosa A. 2013; Systematic and biotechnological aspects of halophilic and halotolerant actinomycetes. Extremophiles 17:1–13 [View Article][PubMed]
    [Google Scholar]
  13. He H., Liu C., Zhao J., Li W., Pan T., Yang L., Wang X., Xiang W. 2014; Streptomyces zhaozhouensis sp. nov., an actinomycete isolated from candelabra aloe (Aloe arborescens Mill). Int J Syst Evol Microbiol 64:1096–1101 [View Article][PubMed]
    [Google Scholar]
  14. Huss V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
    [Google Scholar]
  15. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  16. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Iliarionov S., Goodfellow M. 2000; Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 50:2031–2036 [View Article][PubMed]
    [Google Scholar]
  17. Kim B. Y., Zucchi T. D., Fiedler H. P., Goodfellow M. 2012a; Streptomyces staurospininus sp. nov., a staurosporine-producing actinomycete. Int J Syst Evol Microbiol 62:966–970 [View Article][PubMed]
    [Google Scholar]
  18. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012b; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  19. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [View Article]
    [Google Scholar]
  20. Kurtböke D. 2012; Biodiscovery from rare actinomycetes: an eco-taxonomical perspective. Appl Microbiol Biotechnol 93:1843–1852 [View Article][PubMed]
    [Google Scholar]
  21. Lai H., Wei X., Jiang Y., Chen X., Li Q., Jiang Y., Jiang C., Gillerman L. 2014; Halopolyspora alba gen. nov., sp. nov., isolated from sediment. Int J Syst Evol Microbiol 64:2775–2780 [View Article][PubMed]
    [Google Scholar]
  22. Lechevalier H. A., Lechevalier M. P. 1970a; A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetes pp 393–405Edited by Prauser H. Jena: Gustav Fischer;
    [Google Scholar]
  23. Lechevalier M. P., Lechevalier H. A. 1970b; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
    [Google Scholar]
  24. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (Special Publication 6) pp 277–284Edited by Dietz A., Thayer J. Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  25. Lechevalier M. P., De Bievre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic Actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [View Article]
    [Google Scholar]
  26. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B195–206 [View Article]
    [Google Scholar]
  27. Manfio G. P., Zakrzewska-Czerwinska J., Atalan E., Goodfellow M. 1995; Towards minimal standards for the description of Streptomyces species. Biotekhnologiya 8:228–237
    [Google Scholar]
  28. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J. 2000; A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-stain-positive bacteria. Lett Appl Microbiol 30:178–182 [View Article][PubMed]
    [Google Scholar]
  29. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  30. Okoro C. K., Brown R., Jones A. L., Andrews B. A., Asenjo J. A., Goodfellow M., Bull A. T. 2009; Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile. Antonie van Leeuwenhoek 95:121–133 [View Article][PubMed]
    [Google Scholar]
  31. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  32. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  33. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  34. Tamura K., Nei M. 1993; Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526[PubMed]
    [Google Scholar]
  35. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  36. Tiwari K., Gupta R. K. 2012; Rare actinomycetes: a potential storehouse for novel antibiotics. Crit Rev Biotechnol 32:108–132 [View Article][PubMed]
    [Google Scholar]
  37. Uchida K., Kudo T., Suzuki K. I., Nakase T. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56 [View Article][PubMed]
    [Google Scholar]
  38. Veyisoglu A., Tatar D., Cetin D., Guven K., Sahin N. 2014; Streptomyces karpasiensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 64:827–832 [View Article][PubMed]
    [Google Scholar]
  39. Waksman S. A. 1967 The Actinomycetes. A Summary of Current Knowledge New York: Ronald;
    [Google Scholar]
  40. Waksman S. A., Henrici A. T. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341[PubMed]
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  42. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp 2452–2492Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  43. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16:176–178
    [Google Scholar]
  44. Xiang W., Liu C., Wang X., Du J., Xi L., Huang Y. 2011; Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol 61:1165–1169 [View Article][PubMed]
    [Google Scholar]
  45. Xie Q. Y., Lin H. P., Li L., Brown R., Goodfellow M., Deng Z., Hong K. 2012; Verrucosispora wenchangensis sp. nov., isolated from mangrove soil. Antonie van Leeuwenhoek 102:1–7 [View Article][PubMed]
    [Google Scholar]
  46. Yokota A., Tamura T., Hasegawa T., Huang L. H. 1993; Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . Int J Syst Bacteriol 43:805–812 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000887
Loading
/content/journal/ijsem/10.1099/ijsem.0.000887
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error