1887

Abstract

A Gram-staining-negative bacterial strain (termed CZ-AZ5) was isolated from a biological filter in a marine recirculating aquaculture system in Tianjin, China. Its taxonomic status was determined using a polyphasic approach. CZ-AZ5 cells were non-spore-forming, non-motile rods, 0.6–0.7 μm wide and 3.0–3.7 μm long. CZ-AZ5 was strictly heterotrophic, aerobic, oxidase-negative and catalase-positive. Growth occurred in the temperature range 20–40 °C (optimal: 30 °C), pH range 6.0–8.5 (optimal: pH 7.5) and salinity range 0–5 % (w/v) NaCl (optimal: 1 %). In phylogenetic analyses based on 16S rRNA gene sequences, CZ-AZ5 was assigned to the family (phylum ) and was clustered with the genera and within this family. It showed highest sequence similarity to ‘ Haliscomenobacter calcifugiens’ (86.2 %), followed by ATCC 23119 (85.7 %) and T-3 (85.6 %). DNA G+C content was 40.1 mol%, the major menaquinone was MK-7, and the major cellular fatty acids (>10 %) were Cω7 and iso-C. Our phenotypic, chemotaxonomic and phylogenetic observations, taken together, led us to conclude that strain CZ-AZ5 represents a novel species and genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CZ-AZ5 ( = CGMCC 1.13179 = JCM 18886).

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2016-03-01
2020-03-29
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. 1990; Basic local alignment search tool. J Mol Biol215:403–410 [CrossRef][PubMed]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B.. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol52:1049–1070[PubMed]
    [Google Scholar]
  3. Chen Z., Lei X., Lai Q., Li Y., Zhang B., Zhang J., Zhang H., Yang L., Zheng W., other authors. 2014; Phaeodactylibacter xiamenensis gen. nov., sp. nov., a member of the family Saprospiraceae isolated from the marine alga Phaeodactylum tricornutum . Int J Syst Evol Microbiol64:3496–3502 [CrossRef][PubMed]
    [Google Scholar]
  4. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D.. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp267–287Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;
    [Google Scholar]
  6. Dong X.-Z., Cai M.-Y.. 2001; Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);
    [Google Scholar]
  7. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol20:406–416 [CrossRef]
    [Google Scholar]
  9. Garrity G. M., Holt J. G.. 2001; Taxonomic outline of the Archaea and Bacteria . In Bergey's Manual of Systematic Bacteriology, 2nd edn.vol. 1 pp155–166Edited by Boone D. R., Castenholz R. W., Garrity G. M.. New York: Springer;[CrossRef]
    [Google Scholar]
  10. Hosoya S., Arunpairojana V., Suwannachart C., Kanjana-Opas A., Yokota A.. 2006; Aureispira marina gen. nov., sp. nov., a gliding, arachidonic acid-containing bacterium isolated from the southern coastline of Thailand. Int J Syst Evol Microbiol56:2931–2935 [CrossRef][PubMed]
    [Google Scholar]
  11. Hosoya S., Arunpairojana V., Suwannachart C., Kanjana-Opas A., Yokota A.. 2007; Aureispira maritima sp. nov., isolated from marine barnacle debris. Int J Syst Evol Microbiol57:1948–1951 [CrossRef][PubMed]
    [Google Scholar]
  12. Khan S. T., Fukunaga Y., Nakagawa Y., Harayama S.. 2007; Emended descriptions of the genus Lewinella and of Lewinella cohaerens, Lewinella nigricans and Lewinella persica, and description of Lewinella lutea sp. nov. and Lewinella marina sp. nov. Int J Syst Evol Microbiol57:2946–2951 [CrossRef][PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  14. Lee S. D.. 2007; Lewinella agarilytica sp. nov., a novel marine bacterium of the phylum Bacteroidetes, isolated from beach sediment. Int J Syst Evol Microbiol57:2814–2818 [CrossRef][PubMed]
    [Google Scholar]
  15. Lei X., Li Y., Wang G., Chen Y., Lai Q., Chen Z., Zhang J., Liao P., Zhu H., other authors. 2015; Phaeodactylibacter luteus sp. nov., isolated from the oleaginous microalga Picochlorum sp. Int J Syst Evol Microbiol65:2666–2670 [CrossRef][PubMed]
    [Google Scholar]
  16. Marmur J., Doty P.. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  17. Mcilroy S. J., Nielsen P. H.. 2014; The family Saprospiraceae . In The Prokaryotes, 4th edn. pp863–889Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F.. New York: Springer;
    [Google Scholar]
  18. Oh H.-M., Lee K., Cho J.-C.. 2009; Lewinella antarctica sp. nov., a marine bacterium isolated from Antarctic seawater. Int J Syst Evol Microbiol59:65–68 [CrossRef][PubMed]
    [Google Scholar]
  19. Reichenbach H.. 1989; Genus Saprospira Gross 1911. In Bergey's Manual of Systematic Bacteriologyvol. 3 pp2077–2082Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G.. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  20. Reichenbach H.. 2006; The genus Saprospira . In The Prokaryotes, 3rd edn.vol. 7 pp591–601Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E.. New York: Springer;[CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  22. Sung H.-R., Lee J.-M., Kim M., Shin K.-S.. 2015; Lewinella xylanilytica sp. nov., a member of the family Saprospiraceae isolated from coastal seawater. Int J Syst Evol Microbiol65:3433–3438 [CrossRef][PubMed]
    [Google Scholar]
  23. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  24. van Veen W. L., van der Kooij D., Geuze E.C.W.A., van der Vlies A. W.. 1973; Investigations on the sheathed bacterium Haliscomenobacter hydrossis gen.n., sp.n., isolated from activated sludge. Antonie van Leeuwenhoek39:207–216 [CrossRef][PubMed]
    [Google Scholar]
  25. Wu C., Lu X., Qin M., Wang Y., Ruan J.. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)]16:176–178
    [Google Scholar]
  26. Yoon J., Katsuta A., Kasai H.. 2012a; Rubidimonas crustatorum gen. nov., sp. nov., a novel member of the family Saprospiraceae isolated from a marine crustacean. Antonie van Leeuwenhoek101:461–467 [CrossRef][PubMed]
    [Google Scholar]
  27. Yoon J., Matsuo Y., Kasai H., Yokota A.. 2012b; Portibacter lacus gen. nov., sp. nov., a new member of the family Saprospiraceae isolated from a saline lake. J Gen Appl Microbiol58:191–197 [CrossRef][PubMed]
    [Google Scholar]
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