1887

Abstract

A Gram-staining-negative bacterial strain (termed CZ-AZ5) was isolated from a biological filter in a marine recirculating aquaculture system in Tianjin, China. Its taxonomic status was determined using a polyphasic approach. CZ-AZ5 cells were non-spore-forming, non-motile rods, 0.6–0.7 μm wide and 3.0–3.7 μm long. CZ-AZ5 was strictly heterotrophic, aerobic, oxidase-negative and catalase-positive. Growth occurred in the temperature range 20–40 °C (optimal: 30 °C), pH range 6.0–8.5 (optimal: pH 7.5) and salinity range 0–5 % (w/v) NaCl (optimal: 1 %). In phylogenetic analyses based on 16S rRNA gene sequences, CZ-AZ5 was assigned to the family (phylum ) and was clustered with the genera and within this family. It showed highest sequence similarity to ‘ Haliscomenobacter calcifugiens’ (86.2 %), followed by ATCC 23119 (85.7 %) and T-3 (85.6 %). DNA G+C content was 40.1 mol%, the major menaquinone was MK-7, and the major cellular fatty acids (>10 %) were Cω7 and iso-C. Our phenotypic, chemotaxonomic and phylogenetic observations, taken together, led us to conclude that strain CZ-AZ5 represents a novel species and genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CZ-AZ5 ( = CGMCC 1.13179 = JCM 18886).

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2016-03-01
2019-10-15
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990;). Basic local alignment search tool. J Mol Biol 215: 403–410 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bernardet J.-F. , Nakagawa Y. , Holmes B. . Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [PubMed].
    [Google Scholar]
  3. Chen Z. , Lei X. , Lai Q. , Li Y. , Zhang B. , Zhang J. , Zhang H. , Yang L. , Zheng W. , other authors . ( 2014;). Phaeodactylibacter xiamenensis gen. nov., sp. nov., a member of the family Saprospiraceae isolated from the marine alga Phaeodactylum tricornutum . Int J Syst Evol Microbiol 64: 3496–3502 [CrossRef] [PubMed].
    [Google Scholar]
  4. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57: 2259–2261 [CrossRef] [PubMed].
    [Google Scholar]
  5. Collins M. D. . ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . London: Academic Press;.
    [Google Scholar]
  6. Dong X.-Z. , Cai M.-Y. . ( 2001;). Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);.
    [Google Scholar]
  7. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  8. Fitch W. M. . ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 20: 406–416 [CrossRef].
    [Google Scholar]
  9. Garrity G. M. , Holt J. G. . ( 2001;). Taxonomic outline of the Archaea and Bacteria . . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 1, pp. 155–166. Edited by Boone D. R. , Castenholz R. W. , Garrity G. M. . New York: Springer;.[CrossRef]
    [Google Scholar]
  10. Hosoya S. , Arunpairojana V. , Suwannachart C. , Kanjana-Opas A. , Yokota A. . ( 2006;). Aureispira marina gen. nov., sp. nov., a gliding, arachidonic acid-containing bacterium isolated from the southern coastline of Thailand. Int J Syst Evol Microbiol 56: 2931–2935 [CrossRef] [PubMed].
    [Google Scholar]
  11. Hosoya S. , Arunpairojana V. , Suwannachart C. , Kanjana-Opas A. , Yokota A. . ( 2007;). Aureispira maritima sp. nov., isolated from marine barnacle debris. Int J Syst Evol Microbiol 57: 1948–1951 [CrossRef] [PubMed].
    [Google Scholar]
  12. Khan S. T. , Fukunaga Y. , Nakagawa Y. , Harayama S. . ( 2007;). Emended descriptions of the genus Lewinella and of Lewinella cohaerens, Lewinella nigricans and Lewinella persica, and description of Lewinella lutea sp. nov. and Lewinella marina sp. nov. Int J Syst Evol Microbiol 57: 2946–2951 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  14. Lee S. D. . ( 2007;). Lewinella agarilytica sp. nov., a novel marine bacterium of the phylum Bacteroidetes, isolated from beach sediment. Int J Syst Evol Microbiol 57: 2814–2818 [CrossRef] [PubMed].
    [Google Scholar]
  15. Lei X. , Li Y. , Wang G. , Chen Y. , Lai Q. , Chen Z. , Zhang J. , Liao P. , Zhu H. , other authors . ( 2015;). Phaeodactylibacter luteus sp. nov., isolated from the oleaginous microalga Picochlorum sp. Int J Syst Evol Microbiol 65: 2666–2670 [CrossRef] [PubMed].
    [Google Scholar]
  16. Marmur J. , Doty P. . ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118 [CrossRef] [PubMed].
    [Google Scholar]
  17. Mcilroy S. J. , Nielsen P. H. . ( 2014;). The family Saprospiraceae . . In The Prokaryotes , 4th edn., pp. 863–889. Edited by Rosenberg E. , DeLong E. F. , Lory S. , Stackebrandt E. , Thompson F. . New York: Springer;.
    [Google Scholar]
  18. Oh H.-M. , Lee K. , Cho J.-C. . ( 2009;). Lewinella antarctica sp. nov., a marine bacterium isolated from Antarctic seawater. Int J Syst Evol Microbiol 59: 65–68 [CrossRef] [PubMed].
    [Google Scholar]
  19. Reichenbach H. . ( 1989;). Genus Saprospira Gross 1911. . In Bergey's Manual of Systematic Bacteriology vol. 3, pp. 2077–2082. Edited by Staley J. T. , Bryant M. P. , Pfennig N. , Holt J. G. . Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  20. Reichenbach H. . ( 2006;). The genus Saprospira . . In The Prokaryotes , 3rd edn. vol. 7, pp. 591–601. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K.-H. , Stackebrandt E. . New York: Springer;.[CrossRef]
    [Google Scholar]
  21. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  22. Sung H.-R. , Lee J.-M. , Kim M. , Shin K.-S. . ( 2015;). Lewinella xylanilytica sp. nov., a member of the family Saprospiraceae isolated from coastal seawater. Int J Syst Evol Microbiol 65: 3433–3438 [CrossRef] [PubMed].
    [Google Scholar]
  23. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  24. van Veen W. L. , van der Kooij D. , Geuze E.C.W.A. , van der Vlies A. W. . ( 1973;). Investigations on the sheathed bacterium Haliscomenobacter hydrossis gen.n., sp.n., isolated from activated sludge. Antonie van Leeuwenhoek 39: 207–216 [CrossRef] [PubMed].
    [Google Scholar]
  25. Wu C. , Lu X. , Qin M. , Wang Y. , Ruan J. . ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16: 176–178.
    [Google Scholar]
  26. Yoon J. , Katsuta A. , Kasai H. . ( 2012a;). Rubidimonas crustatorum gen. nov., sp. nov., a novel member of the family Saprospiraceae isolated from a marine crustacean. Antonie van Leeuwenhoek 101: 461–467 [CrossRef] [PubMed].
    [Google Scholar]
  27. Yoon J. , Matsuo Y. , Kasai H. , Yokota A. . ( 2012b;). Portibacter lacus gen. nov., sp. nov., a new member of the family Saprospiraceae isolated from a saline lake. J Gen Appl Microbiol 58: 191–197 [CrossRef] [PubMed].
    [Google Scholar]
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