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Abstract

A biofilm-forming, Gram-stain-negative, aerobic, catalase-positive but oxidase-negative strain, designated CT6, was isolated from the microbial mats (∼45 °C) of a hot water spring, located within the Himalayan ranges at Manikaran, Himachal Pradesh, India. Strain CT6 formed white, smooth colonies with irregular margins. Transmission electron microscopy revealed coccoid, non-flagellated cells with wavy boundaries. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CT6 belongs to the genus with a sequence similarity value of 95.4 % to the sole member of this genus, ATCC 11041. Strain CT6 was found to have phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The major cellular fatty acids were C, summed feature 8 (Cω7 and/or Cω6), C, Cω8 cyclo and summed feature 3 (Cω7 and/or Cω6). The major respiratory quinone was ubiquinone-8. The major polyamines were putrescine, spermidine and the betaproteobacterial-specific 2-hydroxyputrescine. The DNA G+C content was 63.5 mol%. Based on the genotypic, phenotypic, physiological and biochemical data, strain CT6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed ( = DSM 100029 = KCTC 42939 = MCC 2711).

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2016-03-01
2020-01-29
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