1887

Abstract

A novel moderately thermophilic, weakly acidophilic, heterotrophic, anaerobic, short-rod bacterium having an outer sheath-like structure (toga) was isolated from a low-salt acidic terrestrial hot spring in Oku-Shiobara, Tochigi, Japan. The strain, designated NAS-01, grew between 30 and 60 °C (optimum 55 °C), and at pH 4.5 and 7.5 (optimum pH 5.5 to 6.0) and could not grow in media with ≥ 1 % NaCl (optimum 0 % NaCl). It utilized Fe(III), thiosulfate or -cystine as electron acceptor for growth, and yeast extract, peptone or a variety of sugars as carbon and energy sources. The major cellular fatty acid was C, and no lipoquinone was detected. Strain NAS-01 contained phospholipids and glycolipids, but not aminolipids. The DNA G+C content was 41.1 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain was included in the phylum , and was closely related to . The 16S rRNA gene sequence similarity between them was 90 %, and they were deeply branched off the rest of the known species. On the basis of the phylogenetic and ecophysiological properties, strain NAS-01 represents a novel species of a new genus in the phylum , for which we propose the name gen. nov., sp. nov. The type strain of the type species is NAS-01 ( = JCM 19762 = DSM 28954). In addition, we propose a new order and family, ord. nov. and fam. nov., respectively, to accommodate the novel genus and the closely related genus .

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2016-02-01
2020-03-28
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References

  1. Achenbach-Richter L., Gupta R., Stetter K. O., Woese C. R.. 1987; Were the original eubacteria thermophiles?. Syst Appl Microbiol9:34–39 [CrossRef][PubMed]
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S.. 1979; Methanogens: reevaluation of a unique biological group. Microbiol Rev43:260–296[PubMed]
    [Google Scholar]
  3. Ben Hania W., Ghodbane R., Postec A., Brochier-Armanet C., Hamdi M., Fardeau M.-L., Ollivier B.. 2011; Cultivation of the first mesophilic representative (“mesotoga”) within the order Thermotogales . Syst Appl Microbiol34:581–585 [CrossRef][PubMed]
    [Google Scholar]
  4. Ben Hania W., Godbane R., Postec A., Hamdi M., Ollivier B., Fardeau M.-L.. 2012; Defluviitoga tunisiensis gen. nov., sp. nov., a thermophilic bacterium isolated from a mesothermic and anaerobic whey digester. Int J Syst Evol Microbiol62:1377–1382 [CrossRef][PubMed]
    [Google Scholar]
  5. Ben Hania W., Postec A., Aüllo T., Ranchou-Peyruse A., Erauso G., Brochier-Armanet C., Hamdi M., Ollivier B., Saint-Laurent S., other authors. 2013; Mesotoga infera sp. nov., a mesophilic member of the order Thermotogales, isolated from an underground gas storage aquifer. Int J Syst Evol Microbiol63:3003–3008 [CrossRef][PubMed]
    [Google Scholar]
  6. Bhandari V., Gupta R. S.. 2014; Molecular signatures for the phylum (class) Thermotogae and a proposal for its division into three orders (Thermotogales, Kosmotogales ord. nov. and Petrotogales ord. nov.) containing four families (Thermotogaceae, Fervidobacteriaceae fam. nov., Kosmotogaceae fam. nov. and Petrotogaceae fam. nov.) and a new genus Pseudothermotoga gen. nov. with five new combinations. Antonie van Leeuwenhoek105:143–168 [CrossRef][PubMed]
    [Google Scholar]
  7. Castresana J.. 2000; Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol17:540–552 [CrossRef][PubMed]
    [Google Scholar]
  8. Davey M. E., Wood W. A., Key R., Nakamura K., Stahl D. A.. 1993; Isolation of three species of Geotoga and Petrotoga: two new genera, representing a new lineage in the bacterial line of descent distantly related to the “Thermotogales”. Syst Appl Microbiol16:191–200 [CrossRef]
    [Google Scholar]
  9. DiPippo J. L., Nesbø C. L., Dahle H., Doolittle W. F., Birkland N.-K., Noll K. M.. 2009; Kosmotoga olearia gen. nov., sp. nov., a thermophilic, anaerobic heterotroph isolated from an oil production fluid. Int J Syst Evol Microbiol59:2991–3000 [CrossRef][PubMed]
    [Google Scholar]
  10. Edgar R. C.. 2004; muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res32:1792–1797 [CrossRef][PubMed]
    [Google Scholar]
  11. Feng Y., Cheng L., Zhang X., Li X., Deng Y., Zhang H.. 2010; Thermococcoides shengliensis gen. nov., sp. nov., a new member of the order Thermotogales isolated from oil-production fluid. Int J Syst Evol Microbiol60:932–937 [CrossRef][PubMed]
    [Google Scholar]
  12. Guindon S., Dufayard J.-F., Lefort V., Anisimova M., Hordijk W., Gascuel O.. 2010; New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol59:307–321 [CrossRef][PubMed]
    [Google Scholar]
  13. Gupta R. S., Bhandari V.. 2011; Phylogeny and molecular signatures for the phylum Thermotogae and its subgroups. Antonie van Leeuwenhoek100:1–34 [CrossRef][PubMed]
    [Google Scholar]
  14. Huber R., Hannig M.. 2006; Thermotogales. In The Prokaryotes, 3rd edn.vol 7 pp899–922Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E.. Proteobacteria: Delta and Epsilon subclasses. Deeply rooting bacteria New York: Springer;[CrossRef]
    [Google Scholar]
  15. Huber R., Langworthy T. A., König H., Thomm M., Woese C. R., Sleytr U. B., Stetter K. O.. 1986; Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90°C. Arch Microbiol144:324–333 [CrossRef]
    [Google Scholar]
  16. Huber R., Woese C. R., Langworthy T. A., Fricke H., Stetter K. O.. 1989; Thermosipho africanus gen. nov., represents a new genus of thermophilic eubacteria within the “Thermotogales”. Syst Appl Microbiol12:32–37 [CrossRef]
    [Google Scholar]
  17. Itoh T., Suzuki K., Nakase T.. 1998; Thermocladium modestius gen. nov., sp. nov., a new genus of rod-shaped, extremely thermophilic crenarchaeote. Int J Syst Bacteriol48:879–887 [CrossRef][PubMed]
    [Google Scholar]
  18. Itoh T., Yamanoi K., Kudo T., Ohkuma M., Takashina T.. 2011; Aciditerrimonas ferrireducens gen. nov., sp. nov., an iron-reducing thermoacidophilic actinobacterium isolated from a solfataric field. Int J Syst Evol Microbiol61:1281–1285 [CrossRef][PubMed]
    [Google Scholar]
  19. Jayasinghearachchi H. S., Lal B.. 2011; Oceanotoga teriensis gen. nov., sp. nov., a thermophilic bacterium isolated from offshore oil-producing wells. Int J Syst Evol Microbiol61:554–560 [CrossRef][PubMed]
    [Google Scholar]
  20. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  21. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  22. Nesbø C. L., Bradnan D. M., Adebusuyi A., Dlutek M., Petrus A. K., Foght J., Doolittle W. F., Noll K. M.. 2012; Mesotoga prima gen. nov., sp. nov., the first described mesophilic species of the Thermotogales . Extremophiles16:387–393 [CrossRef][PubMed]
    [Google Scholar]
  23. Nishida H., Beppu T., Ueda K.. 2011; Whole-genome comparison clarifies close phylogenetic relationships between the phyla Dictyoglomi and Thermotogae . Genomics98:370–375 [CrossRef][PubMed]
    [Google Scholar]
  24. Nunoura T., Hirai M., Imachi H., Miyazaki M., Makita H., Hirayama H., Furushima Y., Yamamoto H., Takai K.. 2010; Kosmotoga arenicorallina sp. nov. a thermophilic and obligately anaerobic heterotroph isolated from a shallow hydrothermal system occurring within a coral reef, southern part of the Yaeyama Archipelago, Japan, reclassification of Thermococcoides shengliensis as Kosmotoga shengliensis comb. nov., and emended description of the genus Kosmotoga . Arch Microbiol192:811–819 [CrossRef][PubMed]
    [Google Scholar]
  25. Patel B. K. C, Morgan H. W., Daniel R. M.. 1985; Fervidobacterium nodosum gen. nov. and spec. nov., a new chemoorganotrophic, caldoactive, anaerobic bacterium. Arch Microbiol141:63–69 [CrossRef]
    [Google Scholar]
  26. Reysenbach A.-L., Liu Y., Lindgren A. R., Wagner I. D., Sislak C. D., Mets A., Schouten S.. 2013; Mesoaciditoga lauensis gen. nov., sp. nov., a moderately thermoacidophilic member of the order Thermotogales from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol63:4724–4729 [CrossRef][PubMed]
    [Google Scholar]
  27. Sinninghe Damsté J. S., Rijpstra W. I. C, Hopmans E. C., Schouten S., Balk M., Stams A. J. M. 2007; Structural characterization of diabolic acid-based tetraester, tetraether and mixed ether/ester, membrane-spanning lipids of bacteria from the order Thermotogales . Arch Microbiol188:629–641 [CrossRef][PubMed]
    [Google Scholar]
  28. Tamaoka J.. 1994; Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics pp463–470Edited by Goodfellow M., O'Donnell A. G.. Chichester: Wiley;
    [Google Scholar]
  29. Wery N., Lesongeur F., Pignet P., Derennes V., Cambon-Bonavita M.-A., Godfroy A., Barbier G.. 2001; Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol51:495–504 [CrossRef][PubMed]
    [Google Scholar]
  30. Zhaxybayeva O., Swithers K. S., Lapierre P., Fournier G. P., Bickhart D. M., DeBoy R. T., Nelson K. E., Nesbø C. L., Doolittle W. F., other authors. 2009; On the chimeric nature, thermophilic origin, and phylogenetic placement of the Thermotogales . Proc Natl Acad Sci U S A106:5865–5870 [CrossRef][PubMed]
    [Google Scholar]
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