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Abstract

A Gram-stain-positive, non-motile, aerobic and terminal-endospore-forming rod-shaped bacterium, strain P5-1, was isolated from the hindgut of a wood-feeding higher termite, sp. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was closely related to CCM 7311 (97.5 % similarity). Growth was observed at 10–40 °C (optimum, 30 °C) and at pH 5.5–9.0 (optimum, pH 7.5). The DNA G+C content of strain P5-1 was 48.9 mol%. Cells contained menaquinone 7 (MK-7) as the sole respiratory quinone and the major fatty acids were anteiso-C and iso-C. The cellular polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified glycolipid and one unidentified aminophospholipid. The diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained within this study, strain P5-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P5-1 ( = CGMCC 1.15178 = NBRC 111536).

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2016-02-01
2021-10-27
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