1887

Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated HJM-3, was isolated from the place where the ocean and a freshwater lake meet at Hwajinpo on the East Sea, South Korea, and subjected to a polyphasic taxonomic study. The novel strain grew optimally at 30–35 °C, at pH 7.0–7.5 and in the presence of 1.0–2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain HJM-3 clustered with the type strain of and the proposed type strain of . Strain HJM-3 exhibited the highest 16S rRNA gene sequence similarity values (each 95.1 %) to these representative strains of and , and sequence similarities of less than 94.9 % to the type strains of other recognized species. Strain HJM-3 contained menaquinone-6 as the predominant isoprenoid quinone, and iso-C, iso-C G and iso-C 3-OH as the major fatty acids. The major polar lipids detected in strain HJM-3 were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain HJM-3 was 34.7 mol%. The fatty acid and polar lipid profiles and DNA G+C content of strain HJM-3 could be distinguished from those of the phylogenetically related taxa. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain HJM-3 is considered to represent a novel genus and species within the family , for which the name gen. nov., sp. nov., is proposed. The type strain is HJM-3 ( = KCTC 42777 = NBRC 111588).

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2016-02-01
2019-10-23
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