1887

Abstract

Two isolates of aerobic, budding, pink-pigmented bacteria, designated strains PX4 and PT1, were isolated from a boreal peat bog and a forested tundra wetland. Cells of these strains were non-motile spheres that occurred singly or in short chains. Novel isolates were capable of growth at pH values between 3.5 and 6.5 (optimum at pH 5.0–5.5) and at temperatures between 6 and 30 °C (optimum at 15–25 °C). Most sugars and a number of polysaccharides including pectin, xylan, lichenin and Phytagel were used as growth substrates. The major fatty acids were C, Cω9 and C; the major polar lipids were phosphocholine and trimethylornithine. The quinone was menaquinone-6, and the G+C content of the DNA was 66 mol%. Strains PX4 and PT1 were members of the order and displayed 93–94 % 16S rRNA gene sequence similarity to , 91–92 % to species of the genus and 90–91 % to . The two novel strains, however, differed from members of these genera by cell morphology, substrate utilization pattern and a number of physiological characteristics. Based on these data, the novel isolates should be considered as representing a novel genus and species of planctomycetes, for which the name gen. nov., sp. nov., is proposed. The type strain is PX4 ( = DSM 28747 = VKM B-2904). We also suggest the establishment of a novel family, fam. nov., to accommodate stalk-free planctomycetes with spherical cells, which can be assembled in short chains, long filaments or shapeless aggregates. This family includes the genera and .

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2016-02-01
2019-12-14
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References

  1. Auch A. F., von Jan M., Klenk H. P., Göker M.. 2010; Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci2:117–134 [CrossRef][PubMed]
    [Google Scholar]
  2. Bondoso J., Albuquerque L., Nobre M. F., Lobo-da-Cunha A., da Costa M. S., Lage O. M.. 2011; Aquisphaera giovannonii gen. nov., sp. nov., a planctomycete isolated from a freshwater aquarium. Int J Syst Evol Microbiol61:2844–2850 [CrossRef][PubMed]
    [Google Scholar]
  3. Dedysh S. N.. 2011; Cultivating uncultured bacteria from northern wetlands: knowledge gained and remaining gaps. Front Microbiol2:184 [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. 1989; phylip – phylogeny inference package (version 3.2). Cladistics5:164–166
    [Google Scholar]
  5. Gerhardt P.. 1981; Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Giovannoni S. J., Schabtach E., Castenholz R. W.. 1987; Isosphaera pallida, gen. and comb. nov., a gliding, budding eubacterium from hot springs. Arch Microbiol147:276–284 [CrossRef]
    [Google Scholar]
  7. Göker M., Cleland D., Saunders E., Lapidus A., Nolan M., Lucas S., Hammon N., Deshpande S., Cheng J.-F., other authors. 2011; Complete genome sequence of Isosphaera pallida type strain (IS1BT). Stand Genomic Sci4:63–71 [CrossRef][PubMed]
    [Google Scholar]
  8. Ivanova A. O., Dedysh S. N.. 2012; Abundance, diversity, and depth distribution of planctomycetes in acidic northern wetlands. Front Microbiol3:5 [CrossRef][PubMed]
    [Google Scholar]
  9. Kulichevskaya I. S., Ivanova A. O., Baulina O. I., Bodelier P. L. E, Damsté J. S., Dedysh S. N.. 2008; Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands. Int J Syst Evol Microbiol58:1186–1193 [CrossRef][PubMed]
    [Google Scholar]
  10. Kulichevskaya I. S., Detkova E. N., Bodelier P. L., Rijpstra I. C., Sinninghe Damsté J. S., Dedysh S. N.. 2012; Singulisphaera rosea sp. nov., a novel planctomycete from acidic Sphagnum peat, and emended description of the genus Singulisphaera. Int J Syst Evol Microbiol62:118–123 [CrossRef][PubMed]
    [Google Scholar]
  11. Kulichevskaya I. S., Ivanova A. A., Detkova E. N., Rijpstra W. I., Sinninghe Damsté J. S., Dedysh S. N.. 2015; Planctomicrobium piriforme gen. nov., sp. nov., a stalked planctomycete from a littoral wetland of a boreal lake. Int J Syst Evol Microbiol65:1659–1665 [CrossRef][PubMed]
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. 2004; arb: a software environment for sequence data. Nucleic Acids Res32:1363–1371 [CrossRef][PubMed]
    [Google Scholar]
  13. Moore E. K., Villanueva L., Hopmans E. C., Rijpstra W. I. C, Mets A., Dedysh S. N., Sinninghe Damsté J. S.. 2015; Abundant trimethylornithine lipids and specific gene sequences are indicative of planctomycete importance at the oxic/anoxic interface in Sphagnum-dominated northern wetlands. Appl Environ Microbiol81:6333–6344 [CrossRef][PubMed]
    [Google Scholar]
  14. Neef A., Amann R., Schlesner H., Schleifer K.-H.. 1998; Monitoring a widespread bacterial group: in situ detection of planctomycetes with 16S rRNA-targeted probes. Microbiology144:3257–3266 [CrossRef][PubMed]
    [Google Scholar]
  15. Scheuner C., Tindall B. J., Lu M., Nolan M., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Huntemann M., other authors. 2014; Complete genome sequence of Planctomyces brasiliensis type strain (DSM 5305T), phylogenomic analysis and reclassification of Planctomycetes including the descriptions of Gimesia gen. nov., Planctopirus gen. nov. and Rubinisphaera gen. nov. and emended descriptions of the order Planctomycetales and the family Planctomycetaceae. Stand Genomic Sci9:10 [CrossRef][PubMed]
    [Google Scholar]
  16. Serkebaeva Y. M., Kim Y., Liesack W., Dedysh S. N.. 2013; Pyrosequencing-based assessment of the bacteria diversity in surface and subsurface peat layers of a northern wetland, with focus on poorly studied phyla and candidate divisions. PLoS One8:e63994 [CrossRef][PubMed]
    [Google Scholar]
  17. Sinninghe Damsté J. S., Rijpstra W. I. C, Hopmans E. C., Weijers J. W. H, Foesel B. U., Overmann J., Dedysh S. N.. 2011; 13,16-Dimethyl octacosanedioic acid (iso-diabolic acid), a common membrane-spanning lipid of Acidobacteria subdivisions 1 and 3. Appl Environ Microbiol77:4147–4154 [CrossRef][PubMed]
    [Google Scholar]
  18. Staley J. T., Fuerst J. A., Giovannoni S., Schlesner H.. et al. 1992; The order Planctomycetales and the genera Planctomyces, Pirellula, Gemmata and Isosphaera. In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn. pp3710–3731Edited by Balows A., Trüper H., Dworkin M., Harder W., Schleifer K. H.. New York: Springer; [CrossRef]
    [Google Scholar]
  19. Ward N.. 2010; Phylum XXV. Planctomycetes Garrity and Holt 2001, 137 emend. Ward. In Bergey's Manual of Systematic Bacteriologyvol 4 pp879–925Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlung B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B.. New York: Springer; [CrossRef]
    [Google Scholar]
  20. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol173:697–703[PubMed]
    [Google Scholar]
  21. Yarza P., Yilmaz P., Pruesse E., Glöckner F. O., Ludwig W., Schleifer K.-H., Whitman W. B., Euzéby J., Amann R., Rosselló-Móra R.. 2014; Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol12:635–645 [CrossRef][PubMed]
    [Google Scholar]
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