1887

Abstract

A Gram-stain-negative, non-flagellated, aerobic and coccoid, ovoid or rod-shaped bacterium, designated AM-4, was isolated from seawater collected from the East Sea, South Korea and subjected to a taxonomic study using a polyphasic approach. The novel strain grew optimally at 30–35 °C, at pH 7.0–8.0 and in the presence of 1.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain AM-4 forms a distinct evolutionary lineage independent of other taxa of the family . Strain AM-4 exhibited 16S rRNA gene sequence similarities of 95.1–95.5 % to the type strains of , and , and of less than 94.9 % to the type strains of other taxa. Strain AM-4 contained Q-10 as the predominant ubiquinone and Cω7 and C as the major fatty acids. The major polar lipids detected in strain AM-4 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified lipid, one unidentified aminolipid and one unidentified glycolipid. The fatty acid and polar lipid profiles of strain AM-4 were distinguished from those of the phylogenetically related taxa. The DNA G+C content of strain AM-4 was 57.2 mol%. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain AM-4 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov., is proposed. The type strain of the type species is AM-4 ( = KCTC 42776 = CECT 8947).

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2016-02-01
2019-10-20
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