1887

Abstract

A total of 13 isolates from healthy freshwater turtles were characterized by genotypic and phenotypic tests. Phylogenetic analysis of partial 16S rRNA and gene sequences showed that the isolates investigated formed a monophyletic group. The closest related species based on 16S rRNA gene sequencing was CCUG 55632 with 94.4 % similarity and the closest related species based on gene sequence comparison was [] CCUG 19802 with 91.5 % similarity. All the investigated isolates exhibited phenotypic characteristics of the family . However, they could be separated from existing genera of the by the following test results: indole, ornithine decarboxylase and Voges–Proskauer positive; and methyl red, urease and PNPG (α-glucosidase) negative. No X- or V-factor requirement was observed. A zone of β-haemolysis surrounded the colonies after 24 h of incubation on bovine blood agar at 37 °C. Acid was produced from -arabinose, dulcitol, -mannitol, sucrose and trehalose. Representative strain ELNT2x had a fatty acid profile that was characteristic for members of the . ELNT2x expressed only one respiratory quinone, ubiquinone-8 (100 %). The DNA G+C content of strain ELNT2x was 42.8 mol%. On the basis of both phylogenetic and phenotypic evidence, it is proposed that the strains should be classified as representatives of a novel species of a new genus, gen. nov., sp. nov. The type strain of is ELNT2x ( = CCUG 65146 = DSM 28140), which was isolated from the oral cavity of a captive eastern long-necked turtle () in Denmark in 2012.

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2016-02-01
2019-10-19
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