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Abstract

An aerobic, Gram-stain-negative, coccobacillus-shaped, non-endospore-forming, pink-pigmented bacterium, designated PN2, was isolated from an olive leaf. The strain grew at 15–35 °C with an optimum temperature for growth at 30 °C, and at pH 5.0–7.5 with an optimum pH for growth at 6.0. Growth was observed in the presence of up to 1.02 % (w/v) NaCl. The major fatty acids were C cyclo ω8, C and Cω7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, unknown aminolipids, an unknown phospholipid and an unknown lipid. The respiratory quinone was ubiquinone-10. The DNA G+C content of strain PN2 was 70.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PN2 was closely related to members of the genus and shared highest similarity with ATCC BAA-692 (96.5 %), subsp. ATCC 49956 (96.2 %) and subsp. ATCC BAA-691 (96.2 %). Furthermore, the DNA–DNA relatedness value between strain PN2 and the closest related species ATCC BAA-692 was 27 %. These data allowed the phenotypic and genotypic differentiation of strain PN2 from its closest phylogenetic neighbour ( ATCC BAA-692). Based on phenotypic and genotypic characteristics, strain PN2 is classified as representing a novel species of the genus for which the name sp. nov. is proposed. The type strain is PN2 ( = BCC 44864 = NBRC 107871).

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2016-01-01
2020-01-26
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