1887

Abstract

An aerobic, Gram-stain-negative, coccobacillus-shaped, non-endospore-forming, pink-pigmented bacterium, designated PN2, was isolated from an olive leaf. The strain grew at 15–35 °C with an optimum temperature for growth at 30 °C, and at pH 5.0–7.5 with an optimum pH for growth at 6.0. Growth was observed in the presence of up to 1.02 % (w/v) NaCl. The major fatty acids were C cyclo ω8, C and Cω7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, unknown aminolipids, an unknown phospholipid and an unknown lipid. The respiratory quinone was ubiquinone-10. The DNA G+C content of strain PN2 was 70.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PN2 was closely related to members of the genus and shared highest similarity with ATCC BAA-692 (96.5 %), subsp. ATCC 49956 (96.2 %) and subsp. ATCC BAA-691 (96.2 %). Furthermore, the DNA–DNA relatedness value between strain PN2 and the closest related species ATCC BAA-692 was 27 %. These data allowed the phenotypic and genotypic differentiation of strain PN2 from its closest phylogenetic neighbour ( ATCC BAA-692). Based on phenotypic and genotypic characteristics, strain PN2 is classified as representing a novel species of the genus for which the name sp. nov. is proposed. The type strain is PN2 ( = BCC 44864 = NBRC 107871).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000748
2016-01-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/1/474.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000748&mimeType=html&fmt=ahah

References

  1. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496[PubMed]
    [Google Scholar]
  2. Chanprame S., Todd J. J., Widholm J. M. 1996; Prevention of pink-pigmented methylotrophic bacteria (Methylobacterium mesophilicum) contamination of plant tissue cultures. Plant Cell Rep 16:222–225 [View Article][PubMed]
    [Google Scholar]
  3. Chen Q., Sun L. N., Zhang X. X., He J., Kwon S. W., Zhang J., Li S. P., Gu J. G. 2014; Roseomonas rhizosphaerae sp. nov., a triazophos-degrading bacterium isolated from soil. Int J Syst Evol Microbiol 64:1127–1133 [View Article][PubMed]
    [Google Scholar]
  4. Corpe W. A. 1985; A method for detecting methylotrophic bacteria on solid surfaces. J Microbiol Methods 3:215–221 [View Article]
    [Google Scholar]
  5. Dong L., Ming H., Yin Y. R., Duan Y. Y., Zhou E. M., Nie G. X., Feng H. G., Liu L., Li W. J. 2014; Roseomonas alkaliterrae sp. nov., isolated from an alkali geothermal soil sample in Tengchong, Yunnan, South-West China. Antonie van Leeuwenhoek 105:899–905 [View Article][PubMed]
    [Google Scholar]
  6. Duke P. B., Jarvis J. D. 1972; The catalase test- a cautionary tale. Med Lab Technol 29:203–204[PubMed]
    [Google Scholar]
  7. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  9. Furuhata K., Miyamoto H., Goto K., Kato Y., Hara M., Fukuyama M. 2008; Roseomonas stagni sp. nov., isolated from pond water in Japan. J Gen Appl Microbiol 54:167–171 [View Article][PubMed]
    [Google Scholar]
  10. Furuhata K., Ishizaki N., Edagawa A., Fukuyama M. 2013; Roseomonas tokyonensis sp. nov. isolated from a biofilm sample obtained from a cooling tower in Tokyo, Japan. Biocontrol Sci 18:205–209 [View Article][PubMed]
    [Google Scholar]
  11. Gallego V., Sánchez-Porro C., García M. T., Ventosa A. 2006; Roseomonas aquatica sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 56:2291–2295 [View Article][PubMed]
    [Google Scholar]
  12. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
    [Google Scholar]
  13. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  14. Han X. Y., Pham A. S., Tarrand J. J., Rolston K. V., Helsel L. O., Levett P. N. 2003; Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov. Am J Clin Pathol 120:256–264 [View Article][PubMed]
    [Google Scholar]
  15. He D., Kim J. K., Jiang X. Y., Park H. Y., Sun C., Yu H. S., Yoon M. H., Kim S. C., Jin F. X., Im W. T. 2014; Roseomonas sediminicola sp. nov., isolated from fresh water. Antonie van Leeuwenhoek 105:191–197 [View Article][PubMed]
    [Google Scholar]
  16. Hsu S. C., Lockwood J. L. 1975; Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29:422–426[PubMed]
    [Google Scholar]
  17. Jiang C. Y., Dai X., Wang B. J., Zhou Y. G., Liu S. J. 2006; Roseomonas lacus sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56:25–28 [View Article][PubMed]
    [Google Scholar]
  18. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. 1984; Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 48:3169–3172 [View Article]
    [Google Scholar]
  19. Kim D. U., Ka J. O. 2014; Roseomonas soli sp. nov., isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Int J Syst Evol Microbiol 64:1024–1029 [View Article][PubMed]
    [Google Scholar]
  20. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  21. Kim S. J., Weon H. Y., Ahn J. H., Hong S. B., Seok S. J., Whang K. S., Kwon S. W. 2013; Roseomonas aerophila sp. nov., isolated from air. Int J Syst Evol Microbiol 63:2334–2337 [View Article][PubMed]
    [Google Scholar]
  22. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  23. Kovács N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [View Article][PubMed]
    [Google Scholar]
  24. Küster E., Williams S. T. 1964; Production of hydrogen sulfide by Streptomycetes and methods for its detection. Appl Microbiol 12:46–52[PubMed]
    [Google Scholar]
  25. Kuraishi H., Katayama-Fujimura Y., Sugiyama J., Yokoyama T. 1985; Ubiquinone systems in fungi. I. Distribution of ubiquinones in the major families of Ascomycetes, Basidiomycetes, and Deuteromycetes, and their taxonomic implications. Trans Mycol Soc Jpn 26:383–395
    [Google Scholar]
  26. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  27. Limtong S., Kaewwichian R., Jindamorakot S., Yongmanitchai W., Nakase T. 2012; Candida wangnamkhiaoensis sp. nov., an anamorphic yeast species in the Hyphopichia clade isolated in Thailand. Antonie van Leeuwenhoek 102:23–28 [View Article][PubMed]
    [Google Scholar]
  28. Lopes A., Espirito Santo C., Grass G., Chung A. P., Morais P. V. 2011; Roseomonas pecuniae sp. nov., isolated from the surface of a copper-alloy coin. Int J Syst Evol Microbiol 61:610–615 [View Article][PubMed]
    [Google Scholar]
  29. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  30. Nei M., Kumar S. 2000 Molecular Evolution and Phylogenetics New York: Oxford University Press;
    [Google Scholar]
  31. Nutaratat P., Srisuk N., Duangmal K., Yurimoto H., Sakai Y., Muramatsu Y., Nakagawa Y. 2013; Roseomonas musae sp. nov., a new bacterium isolated from a banana phyllosphere. Antonie van Leeuwenhoek 103:617–624 [View Article][PubMed]
    [Google Scholar]
  32. Rihs J. D., Brenner D. J., Weaver R. E., Steigerwalt A. G., Hollis D. G., Yu V. L. 1993; Roseomonas, a new genus associated with bacteremia and other human infections. J Clin Microbiol 31:3275–3283[PubMed]
    [Google Scholar]
  33. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  34. Sánchez-Porro C., Gallego V., Busse H.-J., Kämpfer P., Ventosa A. 2009; Transfer of Teichococcus ludipueritiae and Muricoccus roseus to the genus Roseomonas, as Roseomonas ludipueritiae comb. nov. and Roseomonas rosea comb. nov., respectively, and emended description of the genus Roseomonas. Int J Syst Evol Microbiol 59:1193–1198 [View Article][PubMed]
    [Google Scholar]
  35. Schaeffer A. B., Fulton M. D. 1933; A simplified method of staining endospores. Science 77:194 [View Article][PubMed]
    [Google Scholar]
  36. Sierra G. 1957; A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek 23:15–22 [View Article][PubMed]
    [Google Scholar]
  37. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  38. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  39. Tonouchi A., Tazawa D. 2014; Roseomonas aceris sp. nov. isolated from a mono maple tree in the Shirakami Mountains in Japan. J Gen Appl Microbiol 60:38–43 [View Article][PubMed]
    [Google Scholar]
  40. Venkata Ramana V., Sasikala Ch., Takaichi S., Ramana ChV. 2010; Roseomonas aestuarii sp. nov., a bacteriochlorophyll-a containing alphaproteobacterium isolated from an estuarine habitat of India. Syst Appl Microbiol 33:198–203 [View Article][PubMed]
    [Google Scholar]
  41. Wang C., Xu X. X., Qu Z., Wang H. L., Lin H. P., Xie Q. Y., Ruan J. S., Hong K. 2011; Micromonospora rhizosphaerae sp. nov., isolated from mangrove rhizosphere soil. Int J Syst Evol Microbiol 61:320–324 [View Article][PubMed]
    [Google Scholar]
  42. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  43. Weyant R. S., Whitney A. M. 2005; Roseomonas . In Bergey's Manual of Systematic Bacteriology, Part C, 2nd edn. vol. 2 pp 88–92Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer; [View Article]
    [Google Scholar]
  44. Yamada Y., Kondo K. 1973; Coenzymes Q system in the classification of the yeast genera Rhodotorula and Cryptococcus, and the yeast-like genera Sporobolomyces and Rhodosporidium . J Gen Appl Microbiol 19:59–77 [View Article]
    [Google Scholar]
  45. Yoo S. H., Weon H. Y., Noh H. J., Hong S. B., Lee C. M., Kim B. Y., Kwon S. W., Go S. J. 2008; Roseomonas aerilata sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 58:1482–1485 [View Article][PubMed]
    [Google Scholar]
  46. Yoon J. H., Kang S. J., Oh H. W., Oh T. K. 2007; Roseomonas terrae sp. nov. Int J Syst Evol Microbiol 57:2485–2488 [View Article][PubMed]
    [Google Scholar]
  47. Zhang Y. Q., Yu L. Y., Wang D., Liu H. Y., Sun C. H., Jiang W., Zhang Y. Q., Li W. J. 2008; Roseomonas vinacea sp. nov., a Gram-negative coccobacillus isolated from a soil sample. Int J Syst Evol Microbiol 58:2070–2074 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000748
Loading
/content/journal/ijsem/10.1099/ijsem.0.000748
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error