1887

Abstract

A Gram-stain-negative, curved rod-shaped and non-pigmented strain, HME8277, was isolated from surface seawater of the Yellow Sea in the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to MED297 (96.4 % 16S rRNA gene sequence similarity), IMCC4489 (96.3 %) and KMM 3655 (95.8 %). The major fatty acids were summed feature 3 (comprising Cω7 and/or Cω6; 43.0 %), C (19.0 %) and summed feature 8 (comprising Cω7 and/or Cω6; 15.0 %). The DNA G+C content was 46.1 mol%. The predominant respiratory quinone was Q-8. The major polar lipids of strain HME8277 comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified phospholipids and four unidentified lipids. On the basis of polyphasic analyses, strain HME8277 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain HME8277 ( = KACC 17315 = CECT 8288). An emended description of the genus is also provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000720
2016-01-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/1/360.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000720&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y.,, Holmes B.. & Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes (2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [PubMed].
    [Google Scholar]
  2. Choi A., Cho J. C.. ( 2010;). Reinekea aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 60: 2813–2817 [CrossRef] [PubMed].
    [Google Scholar]
  3. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  4. Gonzalez J. M., Saiz-Jimenez C.. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4: 770–773 [CrossRef] [PubMed].
    [Google Scholar]
  5. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  6. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 125–175. Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;.
    [Google Scholar]
  7. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  8. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  9. Pinhassi J., Pujalte M. J., Macián M. C., Lekunberri I., González J. M., Pedrós-Alió C., Arahal D. R.. ( 2007;). Reinekea blandensis sp. nov., a marine, genome-sequenced gammaproteobacterium. Int J Syst Evol Microbiol 57: 2370–2375 [CrossRef] [PubMed].
    [Google Scholar]
  10. Romanenko L. A., Schumann P., Rohde M., Mikhailov V. V., Stackebrandt E.. ( 2004;). Reinekea marinisedimentorum gen. nov., sp. nov., a novel gammaproteobacterium from marine coastal sediments. Int J Syst Evol Microbiol 54: 669–673 [CrossRef] [PubMed].
    [Google Scholar]
  11. Rosselló-Mora R., Amann R.. ( 2001;). The species concept for prokaryotes. FEMS Microbiol Rev 25: 39–67 [CrossRef] [PubMed].
    [Google Scholar]
  12. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101. Newark, DE: MIDI Inc.
    [Google Scholar]
  13. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849 [CrossRef].
    [Google Scholar]
  14. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  15. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000720
Loading
/content/journal/ijsem/10.1099/ijsem.0.000720
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error