1887

Abstract

A novel orange-pigmented bacterial strain, designated HME6815, was isolated from wetland in Jeju Island, Republic of Korea. The cells were Gram stain-negative, non-motile, strictly aerobic and rod-shaped. Optimal growth occurred at 30 °C and pH 7.0 on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HME6815 formed a distinct phyletic lineage within the family and was most closely related to members of the genera , , , and with 92.3–94.3 % 16S rRNA gene sequence similarity. The major cellular fatty acids were iso-C , iso-C 3-OH, summed feature 3 (C ω7 and/or C ω6) and iso-C . The only respiratory quinone was MK-7. Polar lipid analysis revealed the presence of phosphatidylethanolamine, four unidentified aminolipids, one unidentified aminophospholipid and three unidentified polar lipids. The DNA G+C content was 38.4 mol%. On the basis of the evidence presented in this study, strain HME6815 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is HME6815 ( = KCTC 23736 = CECT 8010).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000719
2016-01-01
2019-09-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/1/326.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000719&mimeType=html&fmt=ahah

References

  1. Albert R. A., Zitomer D., Dollhopf M., Schauer-Gimenez A. E., Struble C., King M., Son S., Langer S., Busse H. J.. ( 2014;). Proposal of Vibrionimonas magnilacihabitans gen. nov., sp. nov., a curved Gram-stain-negative bacterium isolated from lake water. Int J Syst Evol Microbiol 64: 613–620 [CrossRef] [PubMed].
    [Google Scholar]
  2. Anders H., Dunfield P. F., Lagutin K., Houghton K. M., Power J. F., Mackenzie A. D., Vyssotski M., Ryan J. L., Hanssen E. G., other authors. ( 2014;). Thermoflavifilum aggregans gen. nov., sp. nov., a thermophilic and slightly halophilic filamentous bacterium from the phylum Bacteroidetes. Int J Syst Evol Microbiol 64: 1264–1270 [CrossRef] [PubMed].
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [PubMed].
    [Google Scholar]
  4. Eder W., Peplies J., Wanner G., Frühling A., Verbarg S.. ( 2015;). Hydrobacter penzbergensis gen. nov., sp. nov., isolated from purified water. Int J Syst Evol Microbiol 65: 920–926 [CrossRef] [PubMed].
    [Google Scholar]
  5. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  7. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. 20: 406–416.
    [Google Scholar]
  8. Gonzalez J. M., Saiz-Jimenez C.. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4: 770–773 [CrossRef] [PubMed].
    [Google Scholar]
  9. Hucker G. J.. ( 1921;). A new modification and application of the gram stain. J Bacteriol 6: 395–397 [PubMed].
    [Google Scholar]
  10. Kämpfer P., Lodders N., Falsen E.. ( 2011;). Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov. Int J Syst Evol Microbiol 61: 518–523 [CrossRef] [PubMed].
    [Google Scholar]
  11. Kang H., Kim H., Lee B. I., Joung Y., Joh K.. ( 2014;). Sediminibacterium goheungense sp. nov., isolated from a freshwater reservoir. Int J Syst Evol Microbiol 64: 1328–1333 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kang J. Y., Chun J., Seo J.-W., Kim C. H., Jahng K. Y.. ( 2015;). Flaviaesturariibacter amylovorans gen. nov., sp. nov., a starch-hydrolysing bacterium, isolated from estuarine water. Int J Syst Evol Microbiol 65: 2209–2214 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kim Y. J., Nguyen N. L., Weon H. Y., Yang D. C.. ( 2013;). Sediminibacterium ginsengisoli sp. nov., isolated from soil of a ginseng field, and emended descriptions of the genus Sediminibacterium and of Sediminibacterium salmoneum. Int J Syst Evol Microbiol 63: 905–912 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kim S. J., Park J. H., Lim J. M., Ahn J. H., Anandham R., Weon H. Y., Kwon S. W.. ( 2014;). Parafilimonas terrae gen. nov., sp. nov., isolated from greenhouse soil. Int J Syst Evol Microbiol 64: 3040–3045 [CrossRef] [PubMed].
    [Google Scholar]
  16. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 125–175. Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;.
    [Google Scholar]
  17. Leandro T., França L., Nobre M. F., Rainey F. A., da Costa M. S.. ( 2013;). Heliimonas saccharivorans gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from a mineral water aquifer, and emended description of Filimonas lacunae. Int J Syst Evol Microbiol 63: 3793–3799 [CrossRef] [PubMed].
    [Google Scholar]
  18. Lee D. G., Park J. M., Kang H., Hong S. Y., Lee K. R., Chang H. B., Trujillo M. E.. ( 2013;). Asinibacterium lactis gen. nov., sp. nov., a member of the family Chitinophagaceae, isolated from donkey (Equus asinus) milk. Int J Syst Evol Microbiol 63: 3180–3185 [CrossRef] [PubMed].
    [Google Scholar]
  19. Madhaiyan M., Poonguzhali S., Senthilkumar M., Pragatheswari D., Lee J. S., Lee K. C.. ( 2015;). Arachidicoccus rhizosphaerae gen. nov., sp. nov., a plant-growth-promoting bacterium in the family Chitinophagaceae isolated from rhizosphere soil. Int J Syst Evol Microbiol 65: 578–586 [CrossRef] [PubMed].
    [Google Scholar]
  20. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  21. Pruesse E., Peplies J., Glöckner F. O.. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28: 1823–1829 [CrossRef] [PubMed].
    [Google Scholar]
  22. Qu J. H., Yuan H. L.. ( 2008;). Sediminibacterium salmoneum gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment of a eutrophic reservoir. Int J Syst Evol Microbiol 58: 2191–2194 [CrossRef] [PubMed].
    [Google Scholar]
  23. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  24. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  25. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  26. Zhang L., Wang Y., Wei L., Wang Y., Shen X., Li S.. ( 2013;). Taibaiella smilacinae gen. nov., sp. nov., an endophytic member of the family Chitinophagaceae isolated from the stem of Smilacina japonica, and emended description of Flavihumibacter petaseus. Int J Syst Evol Microbiol 63: 3769–3776 [CrossRef] [PubMed].
    [Google Scholar]
  27. Zhao R., Chen X. Y., Li X. D., Tian Y., Kong B. H., Chen Z. L., Li Y. H.. ( 2014;). Cnuella takakiae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides. Int J Syst Evol Microbiol 64: 607–612 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000719
Loading
/content/journal/ijsem/10.1099/ijsem.0.000719
Loading

Data & Media loading...

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error