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Abstract

A Gram-stain-negative, non-spore-forming, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, RA1-3, was isolated from a sea squirt () collected from the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain RA1-3 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain RA1-3 clustered with the type strains of three species of the genus , showing 97.54–98.41 % 16S rRNA gene sequence similarity. Sequence similarities to other recognized species were less than 96.97 %. Strain RA1-3 contained Q-10 as the predominant ubiquinone and Cω7 as the major fatty acid. The major polar lipids of strain RA1-3 were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain RA1-3 was 56 mol% and DNA–DNA relatedness values with the type strains of , and were 13–24 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain RA1-3 is separated from other recognized species of the genus . On the basis of the data presented, strain RA1-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RA1-3 ( = KCTC 42605 = CECT 8868).

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2016-01-01
2019-10-14
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