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Abstract

A novel obligately halophilic, facultatively alkaliphilic actinobacterium, designated EGI 80759, was isolated from the rhizosphere of Willd, Karamay, Xinjiang province, north-west China. Cells of strain EGI 80759 were Gram-stain-positive, non-motile and non-endospore-forming rods. Strain EGI 80759 showed obligately halophilic growth with a tolerance to 8–25 % (w/v) NaCl (optimum growth at 10–12 %, w/v) and facultatively alkaliphilic growth within the pH range 7.0–11.0 (optimum growth at pH 9.0–10.0). Cell-wall hydrolysates of the isolate contained -diaminopimelic acid (peptidoglycan type A1γ), with glucose, glucosamine, ribose and mannose as the major sugars. The major fatty acids identified were 10-methyl-C, Cω8 and C. The predominant menaquinone was MK-9(H). The G+C content of the genomic DNA was 72.1 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain EGI 80759 clustered with members of the class and showed highest 16S rRNA gene sequence similarities with F10 (90.3 %) and ANL-iso2 (88.1 %). On the basis of the data obtained from phenotypic and chemotaxonomic studies and the phylogenetic analysis, the isolate is proposed to be a representative of a novel genus and a novel species, gen. nov., sp. nov., of a proposed novel family, fam. nov., and order, ord. nov., within the class . The type strain of the type species, , is EGI 80759 ( = CGMCC 1.14997 = KCTC 39588).

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2016-01-01
2021-07-23
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