1887

Abstract

Two novel, Gram-stain-variable, moderately thermophilic, acidophilic, rod-shaped, endospore-forming bacteria, G45-16 and G45-17, were isolated from acid mine water of Zijin copper mine in Fujian Province, China. Phylogenetic analysis of 16S rRNA gene sequences showed that they were closely related to ATCC 49025 with sequence similarities of 96.8 %. Cells grew aerobically at 20–45 °C (optimum, 40 °C), at pH 2.5–5.5 (optimum, pH 3.5) and in the presence of 0–4.0 % (w/v) NaCl. Strains contained MK-7 as the major menaquinone and the major cellular fatty acids were ω-cyclohexane C and ω-cyclohexane C. The DNA G+C content was 51.3 and 49.8 mol% ( ) for G45-16 and G45-17, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons with their relatives and DNA–DNA relatedness values, it is concluded that strains G45-16 and G45-17 represent a novel species within the genus , for which the name sp. nov. is proposed; the type strain is G45-16 ( = CGMCC 1.15049 = NBRC 111483).

Funding
This study was supported by the:
  • , National Nature Science Foundation of China , (Award (2014CB846000))
  • , Open Funding Project of the State Key Laboratory of Bioreactor Engineering , (Award 31171234)
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2015-12-01
2020-09-30
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