1887

Abstract

A Gram-stain-negative, non-spore-forming and coccus-shaped bacterial strain, designated 4DR5, was isolated from freshwater and its taxonomic position was investigated using a polyphasic approach. Growth occurred at 10–40 °C (optimum 30 °C), at pH 6–9 (optimum pH 7) and in the presence of 0–0.4 % (w/v) NaCl (optimum 0 %) on R2A agar. On the basis of 16S rRNA gene sequence similarity, strain 4DR5 was assigned to the family of the class , and its closest related taxa were species of the genera (93.67 % sequence similarity), (93.07 %), (92.31–92.38 %), (91.79 %) and (90.24–91.23 %). The predominant isoprenoid quinone detected in strain 4DR5 was Q-10. The major cellular fatty acids were a summed feature consisting of Cω7 and/or Cω6, one consisting of Cω7 and/or Cω6, and C. The major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of the strain was 61.2 mol%. The phylogenetic, chemotaxonomic and biochemical data not only supported the affiliation of strain 4DR5 to the family , but also separated it from other established genera within the family. Therefore, the novel isolate evidently represents a novel species of a new genus of , for which the name gen. nov., sp. nov. is proposed. The type strain of is 4DR5 ( = KCTC 32475 = JCM 19370).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000694
2016-01-01
2024-11-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/1/201.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000694&mimeType=html&fmt=ahah

References

  1. Bogan B. W., Sullivan W. R., Kayser K. J., Derr K. D., Aldrich H. C., Paterek J. R. 2003; Alkanindiges illinoisensis gen. nov., sp. nov., an obligately hydrocarbonoclastic, aerobic squalane-degrading bacterium isolated from oilfield soils. Int J Syst Evol Microbiol 53:1389–1395 [View Article][PubMed]
    [Google Scholar]
  2. Bouvet P. J., Grimont P. A. 1986; Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii . Int J Syst Bacteriol 36:228–240 [View Article]
    [Google Scholar]
  3. Brisou J., Prévot A. R. 1954; [Studies on bacterial taxonomy. X. The revision of species under Acromobacter group.]. Ann Inst Pasteur (Paris) 86:722–728 (in French). [PubMed]
    [Google Scholar]
  4. Carr E. L., Kämpfer P., Patel B. K., Gürtler V., Seviour R. J. 2003; Seven novel species of Acinetobacter isolated from activated sludge. Int J Syst Evol Microbiol 53:953–963 [View Article][PubMed]
    [Google Scholar]
  5. Catlin B. W. 1970; Transfer of the organism named Neisseria catarrhalis to Branhamella gen. nov. Int J Syst Bacteriol 20:155–159 [View Article]
    [Google Scholar]
  6. Franzmann P., Skerman V. 1981; Agitococcus lubricus gen. nov. sp. nov., a lipolytic, twitching coccus from freshwater. Int J Syst Bacteriol 31:177–183 [View Article]
    [Google Scholar]
  7. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [View Article][PubMed]
    [Google Scholar]
  8. Jeon Y.-S., Lee K., Park S.-C., Kim B.-S., Cho Y.-J., Ha S.-M., Chun J. 2014; EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 64:689–691 [View Article][PubMed]
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132Edited by Munro H. N. New York: Academic Press; [CrossRef]
    [Google Scholar]
  10. Juni E., Bøvre K. 2005; Family II. Moraxellaceae Rossau, Van Landschoot, Gillis and De Ley 1991, 317VP . In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol. 2 pp 411–441Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  11. Juni E., Heym G. A. 1986; Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36:388–391 [View Article]
    [Google Scholar]
  12. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [View Article][PubMed]
    [Google Scholar]
  13. Lwoff A. 1939; Revision et demembrement des Hemophilae; le genre Moraxella nov. gen. Ann Inst Pasteur (Paris) 62:168–176
    [Google Scholar]
  14. Malhotra J., Anand S., Jindal S., Rajagopal R., Lal R. 2012; Acinetobacter indicus sp. nov., isolated from a hexachlorocyclohexane dump site. Int J Syst Evol Microbiol 62:2883–2890 [View Article][PubMed]
    [Google Scholar]
  15. Minnikin D., Patel P., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  16. Nemec A., De Baere T., Tjernberg I., Vaneechoutte M., van der Reijden T. J., Dijkshoorn L. 2001; Acinetobacter ursingii sp. nov. and Acinetobacter schindleri sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 51:1891–1899 [View Article][PubMed]
    [Google Scholar]
  17. Nemec A., Dijkshoorn L., Cleenwerck I., De Baere T., Janssens D., Van Der Reijden T. J., Ježek P., Vaneechoutte M. 2003; Acinetobacter parvus sp. nov., a small-colony-forming species isolated from human clinical specimens. Int J Syst Evol Microbiol 53:1563–1567 [View Article][PubMed]
    [Google Scholar]
  18. Nishimura Y., Ino T., Iizuka H. 1988; Acinetobacter radioresistens sp. nov. isolated from cotton and soil. Int J Syst Bacteriol 38:209–211 [View Article]
    [Google Scholar]
  19. Oh K.-H., Lee S.-Y., Lee M.-H., Oh T.-K., Yoon J.-H. 2011; Paraperlucidibaca baekdonensis gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 61:1382–1385 [View Article][PubMed]
    [Google Scholar]
  20. Rossau R., Van Landschoot A., Gillis M., De Ley J. 1991; Taxonomy of Moraxellaceae fam. nov., a new bacterial family to accommodate the genera Moraxella, Acinetobacter, and Psychrobacter and related organisms. Int J Syst Bacteriol 41:310–319 [View Article]
    [Google Scholar]
  21. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  22. Song J., Choo Y.-J., Cho J.-C. 2008; Perlucidibaca piscinae gen. nov., sp. nov., a freshwater bacterium belonging to the family Moraxellaceae . Int J Syst Evol Microbiol 58:97–102 [View Article][PubMed]
    [Google Scholar]
  23. Staley J. T., Irgens R. L., Brenner D. J. 1987; Enhydrobacter aerosaccus gen. nov., sp. nov., a gas-vacuolated, facultatively anaerobic, heterotrophic rod. Int J Syst Bacteriol 37:289–291 [View Article]
    [Google Scholar]
  24. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  25. Yoon J.-H., Park S., Jung Y.-T., Lee J.-S., Lee K.-C. 2013; Paraperlucidibaca wandonensis sp. nov., isolated from seawater, and emended description of the genus Paraperlucidibaca Oh et al. 2011. Int J Syst Evol Microbiol 63:4113–4117 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.000694
Loading
/content/journal/ijsem/10.1099/ijsem.0.000694
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error