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Abstract

A Gram-stain-negative, non-spore-forming and coccus-shaped bacterial strain, designated 4DR5, was isolated from freshwater and its taxonomic position was investigated using a polyphasic approach. Growth occurred at 10–40 °C (optimum 30 °C), at pH 6–9 (optimum pH 7) and in the presence of 0–0.4 % (w/v) NaCl (optimum 0 %) on R2A agar. On the basis of 16S rRNA gene sequence similarity, strain 4DR5 was assigned to the family of the class , and its closest related taxa were species of the genera (93.67 % sequence similarity), (93.07 %), (92.31–92.38 %), (91.79 %) and (90.24–91.23 %). The predominant isoprenoid quinone detected in strain 4DR5 was Q-10. The major cellular fatty acids were a summed feature consisting of Cω7 and/or Cω6, one consisting of Cω7 and/or Cω6, and C. The major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of the strain was 61.2 mol%. The phylogenetic, chemotaxonomic and biochemical data not only supported the affiliation of strain 4DR5 to the family , but also separated it from other established genera within the family. Therefore, the novel isolate evidently represents a novel species of a new genus of , for which the name gen. nov., sp. nov. is proposed. The type strain of is 4DR5 ( = KCTC 32475 = JCM 19370).

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2016-01-01
2020-11-25
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