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Abstract

A yellowish brown, phototrophic, purple non-sulfur bacterium, strain JA924, was isolated in pure culture from a brackish water sample collected from an estuary. Single cells were oval to rod-shaped, non-motile and Gram-stain-negative and had a vesicular architecture of intracellular photosynthetic membranes. Bacteriochlorophyll- and carotenoids of the spheroidene series were present as photosynthetic pigments. Photolithoautotrophy, chemo-organoheterotrophy and photo-organoheterotrophy were the growth modes observed. Strain JA924 had complex growth requirements. Strain JA924 was mesophilic and moderately halophilic. The DNA G+C content was 64 mol% (HPLC). The major cellular fatty acids were Cω7/Cω6, C and C. The major quinone was ubiquinone-10 (Q-10). Phosphatidylglycerol, phosphatidylethanolamine, sulfolipid and an aminolipid were the main polar lipids of strain JA924. EzTaxon-e searches based on the 16S rRNA gene sequence of JA924 revealed highest similarity with AK41 (98.19 %) and other members of the genus ( < 95.71 %). Strain JA924 was further identified to be distantly related to AK41 ( < 29 % based on DNA–DNA hybridization and Δ (>5 °C). Phenotypic, chemotaxonomic and molecular differences indicate that strain JA924 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JA924 ( = LMG 29031 = KCTC 15485).

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2016-01-01
2021-10-24
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