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Abstract

A yellowish brown, phototrophic, purple non-sulfur bacterium, strain JA924, was isolated in pure culture from a brackish water sample collected from an estuary. Single cells were oval to rod-shaped, non-motile and Gram-stain-negative and had a vesicular architecture of intracellular photosynthetic membranes. Bacteriochlorophyll- and carotenoids of the spheroidene series were present as photosynthetic pigments. Photolithoautotrophy, chemo-organoheterotrophy and photo-organoheterotrophy were the growth modes observed. Strain JA924 had complex growth requirements. Strain JA924 was mesophilic and moderately halophilic. The DNA G+C content was 64 mol% (HPLC). The major cellular fatty acids were Cω7/Cω6, C and C. The major quinone was ubiquinone-10 (Q-10). Phosphatidylglycerol, phosphatidylethanolamine, sulfolipid and an aminolipid were the main polar lipids of strain JA924. EzTaxon-e searches based on the 16S rRNA gene sequence of JA924 revealed highest similarity with AK41 (98.19 %) and other members of the genus ( < 95.71 %). Strain JA924 was further identified to be distantly related to AK41 ( < 29 % based on DNA–DNA hybridization and Δ (>5 °C). Phenotypic, chemotaxonomic and molecular differences indicate that strain JA924 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JA924 ( = LMG 29031 = KCTC 15485).

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2016-01-01
2024-12-06
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References

  1. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  2. Gonzalez J. M., Saiz-Jimenez C. 2005; A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. Extremophiles 9:75–79 [View Article][PubMed]
    [Google Scholar]
  3. Hanada S., Takaichi S., Matsuura K., Nakamura K. 2002; Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52:187–193 [View Article][PubMed]
    [Google Scholar]
  4. Hiraishi A., Hoshino Y. 1984; Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 30:435–448 [View Article]
    [Google Scholar]
  5. Hiraishi A., Ueda Y. 1995; Isolation and characterization of Rhodovulum strictum sp. nov. and some other purple nonsulfur bacteria from colored blooms in tidal and seawater pools. Int J Syst Bacteriol 45:319–326 [View Article][PubMed]
    [Google Scholar]
  6. Hiraishi A., Hoshino Y., Kitamura H. 1984; Isoprenoid quinone composition in the classification of Rhodospirillaceae . J Gen Appl Microbiol 30:197–210 [View Article]
    [Google Scholar]
  7. Imhoff J. F. 1984; Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 25:85–89 [View Article]
    [Google Scholar]
  8. Imhoff J. F. 2005; Genus XIV. Rhodovulum. Hiraishi & Ueda 1994a, 21VP. In. Bergey's Manual of Systematic Bacteriology, 2nd edn. vol. 2 pp 205–209Edited by Brenner D. J., Krieg R., Staley J. T., Garrity G. M. New York: Springer; [CrossRef]
    [Google Scholar]
  9. Imhoff J. F., Caumette P. 2004; Recommended standards for the description of new species of anoxygenic phototrophic bacteria. Int J Syst Evol Microbiol 54:1415–1421 [View Article][PubMed]
    [Google Scholar]
  10. Kates M. 1972; Techniques in lipidology. In Laboratory Techniques in Biochemistry and Molecular Biology vol 3 pp 355–356Edited by Work T. S., Work E. New York: Elsevier;
    [Google Scholar]
  11. Kates M. 1986; Techniques of lipidology: isolation, analysis and identification of lipids. In Laboratory Techniques in Biochemistry and Molecular Biology vol 3 pp 100–112Edited by Burdon R. H., van Knippenberg P. H. Amsterdam: Elsevier;
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  13. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  14. Lakshmi K.V.N.S., Sasikala Ch., Takaichi S., Ramana ChV. 2011; Phaeospirillum oryzae sp. nov., a spheroplast-forming, phototrophic alphaproteobacterium from a paddy soil. Int J Syst Evol Microbiol 61:1656–1661 [View Article][PubMed]
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  17. Norris J. R., Ribbons D. W. 1972; The Determination of the Molecular Weight of DNA Per Bacterial Nucleoid.. Methods Microbiol 5A:1–385
    [Google Scholar]
  18. Oren A., Duker S., Ritter S. 1996; The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138:135–140 [View Article]
    [Google Scholar]
  19. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [View Article][PubMed]
    [Google Scholar]
  20. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  21. Shivali K., Ramana V. V., Ramaprasad E. V. V., Sasikala Ch., Ramana ChV. 2011; Marichromatium litoris sp. nov. and Marichromatium chrysaorae sp. nov. isolated from beach sand and from a jelly fish (Chrysaora colorata). Syst Appl Microbiol 34:600–605 [View Article][PubMed]
    [Google Scholar]
  22. Soto C. Y., Cama M., Gibert I., Luquin M. 2000; Application of an easy and reliable method for sulfolipid-I detection in the study of its distribution in Mycobacterium tuberculosis strains. FEMS Microbiol Lett 187:103–107 [View Article][PubMed]
    [Google Scholar]
  23. Stackebrandt E., Goebel B. M. 1994; A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  24. Subhash Y., Sasikala Ch., Ramana ChV. 2013a; Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. Int J Syst Evol Microbiol 63:3463–3469 [View Article][PubMed]
    [Google Scholar]
  25. Subhash Y., Tushar L., Sasikala Ch., Ramana ChV. 2013b; Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 63:4524–4532 [View Article][PubMed]
    [Google Scholar]
  26. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis (mega) version 6. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  27. Tindall B. J. 1990a; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  28. Tindall B. J. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  29. Tourova T. P., Antonov A. S. 1988; Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19:333–355 [View Article]
    [Google Scholar]
  30. Trüper H. G., Pfennig N. 1981; Isolation of members of the families Chromatiaceae and Chlorobiaceae. In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria pp 279–289Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler P., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  32. Wyatt G. R. 1953; The quantitative composition of deoxypentose nucleic acids as related to the newly proposed structure. Cold Spring Harb Symp Quant Biol 18:133–134 [View Article][PubMed]
    [Google Scholar]
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