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Abstract

A novel actinomycete, designated strain 2902at01 was isolated from soil collected at a mangrove forest in Zhanjiang, Guangdong province, China. The strain was identified using a polyphasic classification method. The 16S rRNA gene sequence of strain 2902at01 showed the highest similarity to Y22 (98.3 %) and TJ2-2 (98.1 %). Phylogenetic analysis based on the gene sequence also clearly showed that the strain was different from any previously discovered species of the genus . The characteristic whole-cell sugars were ribose and xylose. The cell-wall hydrolysates contained alanine, asparagine, glycine and -diaminopimelic acid. MK-10(H) and MK-10(H) were the major menaquinones of the novel strain. The predominant fatty acids were iso-C, anteiso-C and iso-C The characteristic polar lipids of strain 2902at01 were phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and diphosphatidylglycerol. The DNA G+C content was 70.2 mol%. DNA–DNA hybridization data combined with other physiological and biochemical features could distinguish strain 2902at01 from the reference strains Y22 and TJ2-2 . On the basis of these phenotypic and genotypic data, strain 2902at01 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2902at01 ( = CCTCC AA2014018 = DSM 45902).

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2015-12-01
2021-10-15
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