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Abstract

A Gram-stain-negative, coccobacilli-shaped bacterium, designated YC6724, was isolated from the rhizosphere of rice in Jinju, Korea. The taxonomy of strain YC6724 was studied using a polyphasic approach. Strain YC6724 grew optimally at 30 °C and pH 7.0–8.0. Comparative 16S rRNA gene sequence analyses showed that the strain was most closely related to 5N26 (98.4 % 16S rRNA gene sequence similarity), TH-G33 (97.3 %) and DS-48 (97.3 %). Sequence similarities with other species of the genus with validly published names were lower than 94.0 %. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain YC6724 formed a distinct phyletic lineage within the genus . Strain YC6724 had DNA–DNA relatedness values of 16.6 %, 44.0 % and 33.2 % with KACC 16376, KACC 12677 and KACC 11678, respectively. The predominant fatty acids of strain YC6724 were Cω7 and/or Cω6, C and C 2-OH. The polar lipid profile contained phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminolipid and two unknown lipids. The G+C content of the genomic DNA was 70.5 mol% and the major quinone was Q-10. Strain YC6724 contained spermidine as the major polyamine. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain YC6724 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YC6724 ( = KCTC 22478 = NBRC 109439).

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2015-12-01
2019-10-23
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