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Abstract

An obligately thermophilic, chemolithotrophic, microaerophilic bacterium, designated strain GBS1, was isolated from the water column of Great Boiling Spring, Nevada, USA. Thiosulfate was required for growth. Although capable of autotrophy, growth of GBS1 was enhanced in the presence of acetate, peptone or Casamino acids. Growth occurred at 70–85 °C with an optimum at 80 °C, at pH 6.50–7.75 with an optimum at pH 7.25, with 0.5–8 % oxygen with an optimum at 1–2 % and with ≤ 200 mM NaCl. The doubling time under optimal growth conditions was 1.3 h, with a final mean cell density of 6.2 ± 0.5 × 10 cells ml. Non-motile, rod-shaped cells 1.4–2.4 × 0.4–0.6 μm in size occurred singly or in pairs. The major cellular fatty acids (>5 % of the total) were Cω9, C, C and C. Phylogenetic analysis of the GBS1 16S rRNA gene sequence indicated an affiliation with and other species of the genus , but determination of 16S rRNA gene sequence similarity ( ≤ 97.10 %) and estimated DNA–DNA hybridization values ( ≤ 18.4 %) with the type strains of recognized species indicate that the novel strain is distinct from described species. Based on phenotypic, genotypic and phylogenetic characteristics, a novel species, sp. nov., is proposed, with GBS1 ( = JCM 19133 = DSM 27162) as the type strain.

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2015-12-01
2024-12-05
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References

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. ( 1979;). Methanogens: reevaluation of a unique biological group. Microbiol Rev 43 260296.
    [Google Scholar]
  2. Blank C. E., Cady S. L., Pace N. R. ( 2002;). Microbial composition of near-boiling silica-depositing thermal springs throughout Yellowstone National Park. Appl Environ Microbiol 68 51235135 [View Article].
    [Google Scholar]
  3. Boyd E. S., Leavitt W. D., Geesey G. G. ( 2009;). CO2 uptake and fixation by a thermoacidophilic microbial community attached to precipitated sulfur in a geothermal spring. Appl Environ Microbiol 75 42894296 [View Article].
    [Google Scholar]
  4. Caldwell S. L., Liu Y., Ferrera I., Beveridge T., Reysenbach A.-L. ( 2010;). Thermocrinis minervae sp. nov., a hydrogen- and sulfur-oxidizing, thermophilic member of the Aquificales from a Costa Rican terrestrial hot spring. Int J Syst Evol Microbiol 60 338343 [View Article].
    [Google Scholar]
  5. Cole J. K., Peacock J. P., Dodsworth J. A., Williams A. J., Thompson D. B., Dong H., Wu G., Hedlund B. P. ( 2013a;). Sediment microbial communities in Great Boiling Spring are controlled by temperature and distinct from water communities. ISME J 7 718729 [View Article].
    [Google Scholar]
  6. Cole J. K., Gieler B. A., Heisler D. L., Palisoc M. M., Williams A. J., Dohnalkova A. C., Ming H., Yu T. T., Dodsworth J. A., other authors. ( 2013b;). Kallotenue papyrolyticum gen. nov., sp. nov., a cellulolytic and filamentous thermophile that represents a novel lineage (Kallotenuales ord. nov., Kallotenuaceae fam. nov.) within the class Chloroflexia . Int J Syst Evol Microbiol 63 46754682 [View Article].
    [Google Scholar]
  7. Connon S. A., Koski A. K., Neal A. L., Wood S. A., Magnuson T. S. ( 2008;). Ecophysiology and geochemistry of microbial arsenic oxidation within a high arsenic, circumneutral hot spring system of the Alvord Desert. FEMS Microbiol Ecol 64 117128 [View Article].
    [Google Scholar]
  8. Costa K. C., Navarro J. B., Shock E. L., Zhang C. L., Soukup D., Hedlund B. P. ( 2009;). Microbiology and geochemistry of great boiling and mud hot springs in the United States Great Basin. Extremophiles 13 447459 [View Article].
    [Google Scholar]
  9. Dodsworth J. A., Gevorkian J., Despujos F., Cole J. K., Murugapiran S. K., Ming H., Li W.-J., Zhang G., Dohnalkova A., Hedlund B. P. ( 2014;). Thermoflexus hugenholtzii gen. nov., sp. nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov. and Thermoflexales ord. nov. Int J Syst Evol Microbiol 64 21192127 [View Article].
    [Google Scholar]
  10. Eder W., Huber R. ( 2002;). New isolates and physiological properties of the Aquificales and description of Thermocrinis albus sp. nov. Extremophiles 6 309318 [View Article].
    [Google Scholar]
  11. Felsenstein J. ( 1989;). phylip – phylogeny inference package (version 3.2). Cladistics 5 164166.
    [Google Scholar]
  12. Hall J. R., Mitchell K. R., Jackson-Weaver O., Kooser A. S., Cron B. R., Crossey L. J., Takacs-Vesbach C. D. ( 2008;). Molecular characterization of the diversity and distribution of a thermal spring microbial community by using rRNA and metabolic genes. Appl Environ Microbiol 74 49104922 [View Article].
    [Google Scholar]
  13. Hedlund B. P., Reysenbach A.-L., Huang L., Ong J. C., Liu Z., Dodsworth J. A., Ahmed R., Williams A. J., Briggs B. R., other authors. ( 2015;). Isolation of diverse members of the Aquificales from geothermal springs in Tengchong. China. Front Microbiol 6 157 [View Article].
    [Google Scholar]
  14. Huber R., Eder W., Heldwein S., Wanner G., Huber H., Rachel R., Stetter K. O. ( 1998;). Thermocrinis ruber gen. nov., sp. nov., a pink-filament-forming hyperthermophilic bactrium isolated from Yellowstone National Park. Appl Environ Microbiol 64 35763583.
    [Google Scholar]
  15. Jahnke L. L., Eder W., Huber R., Hope J. M., Hinrichs K. U., Hayes J. M., Des Marais D. J., Cady S. L., Summons R. E. ( 2001;). Signature lipids and stable carbon isotope analyses of Octopus Spring hyperthermophilic communities compared with those of Aquificales representatives. Appl Environ Microbiol 67 51795189 [View Article].
    [Google Scholar]
  16. Johnson D. B., Rolfe S., Hallberg K. B., Iversen E. ( 2001;). Isolation and phylogenetic characterization of acidophilic microorganisms indigenous to acidic drainage waters at an abandoned Norwegian copper mine. Environ Microbiol 3 630637 [View Article].
    [Google Scholar]
  17. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article].
    [Google Scholar]
  18. Meier-Kolthoff J. P., Auch A. F., Klenk H.-P., Göker M. ( 2013;). Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14 60 [View Article].
    [Google Scholar]
  19. Meyer-Dombard D. R., Shock E. L., Amend J. P. ( 2005;). Archaeal and bacterial communities in geochemically diverse hot springs of Yellowstone National Park, USA. Geobiology 3 211227 [View Article].
    [Google Scholar]
  20. Meyer-Dombard D. R., Swingley W., Raymond J., Havig J., Shock E. L., Summons R. E. ( 2011;). Hydrothermal ecotones and streamer biofilm communities in the Lower Geyser Basin, Yellowstone National Park. Environ Microbiol 13 22162231 [View Article].
    [Google Scholar]
  21. Quast C., Pruesse E., Yilmaz P., Gerken J., Schweer T., Yarza P., Peplies J., Glöckner F. O., ribosomal R.N.A. ( 2013;). The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41 (D1), D590D596 [View Article].
    [Google Scholar]
  22. Reysenbach A. L., Wickham G. S., Pace N. R. ( 1994;). Phylogenetic analysis of the hyperthermophilic pink filament community in Octopus Spring, Yellowstone National Park. Appl Environ Microbiol 60 21132119.
    [Google Scholar]
  23. Schloss P. D., Westcott S. L., Ryabin T., Hall J. R., Hartmann M., Hollister E. B., Lesniewski R. A., Oakley B. B., Parks D. H., other authors. ( 2009;). Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75 75377541 [View Article].
    [Google Scholar]
  24. Spear J. R., Walker J. J., McCollom T. M., Pace N. R. ( 2005;). Hydrogen and bioenergetics in the Yellowstone geothermal ecosystem. Proc Natl Acad Sci U S A 102 25552560 [View Article].
    [Google Scholar]
  25. Stamatakis A. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22 26882690 [View Article].
    [Google Scholar]
  26. Sutcliffe I. C. ( 2011;). Cell envelope architecture in the Chloroflexi: a shifting frontline in a phylogenetic turf war. Environ Microbiol 13 279282 [View Article].
    [Google Scholar]
  27. Takacs C. D., Ehringer M., Favre R., Cermola M., Eggertsson G., Palsdottir A., Reysenbach A.-L. ( 2001;). Phylogenetic characterization of the blue filamentous bacterial community from an Icelandic geothermal spring. FEMS Microbiol Ecol 35 123128 [View Article].
    [Google Scholar]
  28. Vick T. J., Dodsworth J. A., Costa K. C., Shock E. L., Hedlund B. P. ( 2010;). Microbiology and geochemistry of Little Hot Creek, a hot spring environment in the Long Valley Caldera. Geobiology 8 140154 [View Article].
    [Google Scholar]
  29. Wirth R., Sikorski J., Brambilla E., Misra M., Lapidus A., Copeland A., Nolan M., Lucas S., Chen F., other authors. ( 2010;). Complete genome sequence of Thermocrinis albus type strain (HI 11/12). Stand Genomic Sci 2 194202 [View Article].
    [Google Scholar]
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