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Abstract

A novel actinomycete, designated strain NEAU-A-2, was isolated from the rhizosphere soil of Prain collected from Heilongjiang province, north-east China. The taxonomic status of this organism was established using a polyphasic approach. The isolate formed irregular sporangia containing motile spores on the substrate mycelium. The whole-cell sugars were xylose and galactose. The predominant menaquinones were MK-9(H), MK-9(H), MK-10(H) and MK-10(H). The major fatty acids were iso-C, iso-C and anteiso-C. The polar lipids were diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids and an unidentified glycolipid. 16S rRNA gene sequence similarity studies showed that strain NEAU-A-2 belongs to the genus with the highest sequence similarities to NBRC 13912 (97.7 % 16S rRNA gene sequence similarity), IMSNU 22125 (97.5 %), MN07-A0368 (97.2 %) and NBRC 108556 (97.2 %); similarities to type strains of other species of this genus were < 97 %. Two tree-making algorithms showed that strain NEAU-A-2 formed a distinct clade with NBRC 13912 and NBRC 108556. However, low DNA–DNA relatedness values allowed the isolate to be differentiated from the above-mentioned two species of the genus . Moreover, strain NEAU-A-2 could also be distinguished from the most closely related species by morphological and physiological characteristics. Therefore, in conclusion, isolate NEAU-A-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-A-2 ( = CGMCC 4.7133 = DSM 46672).

Funding
This study was supported by the:
  • the National Outstanding Youth Foundation (Award 31225024)
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2015-12-01
2021-04-13
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