1887

Abstract

A novel actinomycete strain, designated LIPI11-2-Ac046, was isolated from a leaf litter sample obtained from Cibodas Botanical Garden, West Java, Indonesia, using the rehydration and centrifugation method. The taxonomic status of this organism was established using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain LIPI11-2-Ac046 had the closest sequence similarities with members of the genus (97.99–98.90 %). The strain grew well on ISP 4 and ISP 5 media and formed sporangia. Spores of this strain were motile. The strain grew in the presence of 0–2 % (w/v) NaCl and the temperature range of 15–28 °C. The cell-wall hydrolysate contained -diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysate contained mannose, glucose, galactose, ribose and xylose, together with one unidentified -methyl-pentose. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H), and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were Cω9, iso-C, C and Cω9. These phenotypic characteristics corresponded to those of the genus . Meanwhile, the results of DNA–DNA hybridization as well as physiological and biochemical analyses distinguished strain LIPI11-2-Ac046 from known members of the genus . On the basis of these data, it is proposed that strain LIPI11-2-Ac046 represents a novel species of the genus , with the name sp. nov. The type strain is LIPI11-2-Ac046 ( = InaCC A457 = NBRC 110976).

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2015-12-01
2019-10-21
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