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Volume 65, Issue Pt_12

sp. nov., isolated from leaf litter in Indonesia Free

Abstract

A novel actinomycete strain, designated LIPI11-2-Ac046, was isolated from a leaf litter sample obtained from Cibodas Botanical Garden, West Java, Indonesia, using the rehydration and centrifugation method. The taxonomic status of this organism was established using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain LIPI11-2-Ac046 had the closest sequence similarities with members of the genus (97.99–98.90 %). The strain grew well on ISP 4 and ISP 5 media and formed sporangia. Spores of this strain were motile. The strain grew in the presence of 0–2 % (w/v) NaCl and the temperature range of 15–28 °C. The cell-wall hydrolysate contained -diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysate contained mannose, glucose, galactose, ribose and xylose, together with one unidentified -methyl-pentose. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H), and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were Cω9, iso-C, C and Cω9. These phenotypic characteristics corresponded to those of the genus . Meanwhile, the results of DNA–DNA hybridization as well as physiological and biochemical analyses distinguished strain LIPI11-2-Ac046 from known members of the genus . On the basis of these data, it is proposed that strain LIPI11-2-Ac046 represents a novel species of the genus , with the name sp. nov. The type strain is LIPI11-2-Ac046 ( = InaCC A457 = NBRC 110976).

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2015-12-01
2024-09-20
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References

  1. Ara I., Tsetseg B., Daram D., Suto M., Ando K. ( 2012;). Cryptosporangium mongoliense sp. nov., isolated from soil. Int J Syst Evol Microbiol 62 2480–2484 [View Article] [PubMed] .
     [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224–229 [View Article].
     [Google Scholar]
  3. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376 [View Article] [PubMed] .
     [Google Scholar]
  4. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  5. Fitch W. M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  6. Hamada M., Yamamura H., Komukai C., Tamura T., Suzuki K., Hayakawa M. ( 2012;). Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium . J Antibiot (Tokyo) 65 427–431 [View Article] [PubMed] .
     [Google Scholar]
  7. Hamada M., Shibata C., Tamura T., Suzuki K. ( 2014;). Agromyces marinus sp. nov., a novel actinobacterium isolated from sea sediment. J Antibiot (Tokyo) 67 703–706 [View Article] [PubMed] .
     [Google Scholar]
  8. Hayakawa M., Nonomura H. ( 1987;). Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65 501–509 [View Article].
     [Google Scholar]
  9. Hayakawa M., Otoguro M., Takeuchi T., Yamazaki T., Iimura Y. ( 2000;). Application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. Antonie van Leeuwenhoek 78 171–185 [View Article] [PubMed] .
     [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed] .
     [Google Scholar]
  11. Lechevalier M. P., De Bièvre C., Lechevalier H. A. ( 1977;). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5 249–260 [View Article].
     [Google Scholar]
  12. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  13. Rayner R. W. ( 1970). A Mycological Colour Chart Kew, UK: Commonwealth Mycological Institute and British Mycological Society;.
     [Google Scholar]
  14. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  15. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  16. Shirling E. B., Gottlieb D. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16 313–340 [View Article].
     [Google Scholar]
  17. Tamura T., Hatano K. ( 2001;). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51 2119–2125 [View Article] [PubMed] .
     [Google Scholar]
  18. Tamura T., Hayakawa M., Hatano K. ( 1998;). A new genus of the order Actinomycetales, Cryptosporangium gen. nov., with descriptions of Cryptosporangium arvum sp. nov. and Cryptosporangium japonicum sp. nov. Int J Syst Bacteriol 48 995–1005 [View Article] [PubMed] .
     [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed] .
     [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P.A.D., Kandler O., Krichevsky M. I., Moore L. H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463–464 [View Article].
     [Google Scholar]
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Cryptosporangium cibodasense sp. nov., isolated from leaf litter in Indonesia
Int J Syst Evol Microbiol 65, 4632 (2015); https://doi.org/10.1099/ijsem.0.000625
/content/journal/ijsem/10.1099/ijsem.0.000625
/content/journal/ijsem/10.1099/ijsem.0.000625
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