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Abstract

An actinomycete, strain K55, was isolated from a composite soil sample from a nickel mine, collected from Yueyang, Shaanxi Province, PR China. Strain K55 showed 16S rRNA gene sequence similarities of 98.73 %–98.51 % to species of the genus , including 232617, 2-30-b(28), 2602GPT1-05, IMSNU 22003, NEAU-MES19, and P0402. This strain harboured -diaminopimelic acid, alanine and glycine as the major cell-wall amino acids, xylose and glucose as the characteristic whole-cell sugars, and iso-C (20.53 %),iso-C (12.74 %), iso-C (12.15 %), anteiso-C (7.97 %), Cω8c (7.49 %) and C (6.63 %) as the dominant fatty acids. The major menaquinones were MK-10(H) and MK-10(H). The phospholipid profile comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol and unknown phosphoglycolipids. The DNA G+C content was 71.4 mol%. A comprehensive analysis of several physiological and biochemical traits and DNA–DNA relatedness indicated that strain K55 was different from closely related species. These phenotypic, genotypic and chemotaxonomic data suggest that strain K55 represents a novel species of the genus , for which the name sp. nov., is proposed. The type strain is K55 ( = JCM 30559 = ACCC19713).

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2015-12-01
2019-12-06
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References

  1. Bérdy J.. ( 2005;). Bioactive microbial metabolites. J Antibiot (Tokyo) 58: 1–26 [CrossRef] [PubMed].
    [Google Scholar]
  2. Chun J., Goodfellow M.. ( 1995;). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45: 240–245 [CrossRef] [PubMed].
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 [CrossRef] [PubMed].
    [Google Scholar]
  4. Eguchi T., Takada N., Nakamura S., Tanaka T., Makino T., Oshima Y.. ( 1993;). Streptomyces bungoensis sp. nov. Int J Syst Bacteriol 43: 794–798 [CrossRef].
    [Google Scholar]
  5. Everest G. J., Meyers P. R.. ( 2013;). Micromonospora equina sp. nov., isolated from soil from a racecourse. Int J Syst Evol Microbiol 63: 879–885 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  7. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  8. Goodfellow M.. ( 1971;). Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69: 33–80 [CrossRef] [PubMed].
    [Google Scholar]
  9. Gordon R. E., Mihm J. M.. ( 1962;). The type species of the genus Nocardia. J Gen Microbiol 27: 1–10 [CrossRef] [PubMed].
    [Google Scholar]
  10. Guo J. K., Lin Y. B., Zhao M. L., Sun R., Wang T. T., Tang M., Wei G. H.. ( 2009;). Streptomyces plumbiresistens sp. nov., a lead-resistant actinomycete isolated from lead-polluted soil in north-west China. Int J Syst Evol Microbiol 59: 1326–1330 [CrossRef] [PubMed].
    [Google Scholar]
  11. Gurovic M.S.V., Müller S., Domin N., Seccareccia I., Nietzsche S., Martin K., Nett M.. ( 2013;). Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil. Int J Syst Evol Microbiol 63: 3812–3817 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42: 989–1005 [CrossRef].
    [Google Scholar]
  13. Kawamoto I.. ( 1989;). Genus Micromonospora in S.T. . In In Bergey's Manual of Systematic Bacteriologyvol. 4, pp. 2442–2450. Edited by Krieg N. R., Parte A., Ludwig W., Whitman W., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D.. Baltimore: Williams & Wilkins;.
    [Google Scholar]
  14. Kelly K. L.. ( 1964;). Inter-Society Color Council-National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;.
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  16. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20: 435–443 [CrossRef].
    [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. A.. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy, pp. 227–291. Edited by Dietz A., Thayer D. W.. Arlington, VA: Society for Industrial Microbiology;.
    [Google Scholar]
  19. Li L., Tang Y. L., Wei B., Xie Q. Y., Deng Z., Hong K.. ( 2013;). Micromonospora sonneratiae sp. nov., isolated from a root of Sonneratia apetala. Int J Syst Evol Microbiol 63: 2383–2388 [CrossRef] [PubMed].
    [Google Scholar]
  20. Lin Y. B., Wang X. Y., Li H. F., Wang N. N., Wang H. X., Tang M., Wei G. H.. ( 2011;). Streptomyces zinciresistens sp. nov., a zinc-resistant actinomycete isolated from soil from a copper and zinc mine. Int J Syst Evol Microbiol 61: 616–620 [CrossRef] [PubMed].
    [Google Scholar]
  21. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118 [CrossRef] [PubMed].
    [Google Scholar]
  22. Minnikin D., O'donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  23. Nimaichand S., Zhang Y. G., Cheng J., Li L., Zhang D. F., Zhou E. M., Dong L., Ningthoujam D. S., Li W. J.. ( 2013;). Micromonospora kangleipakensis sp. nov., isolated from a sample of limestone quarry. Int J Syst Evol Microbiol 63: 4546–4551 [CrossRef] [PubMed].
    [Google Scholar]
  24. Ørskov J.. ( 1923;). Investigations into the Morphology of the Ray Fungi Copenhagen: Levin and Munksgaard Publishers;.
    [Google Scholar]
  25. Ren J., Li L., Wei B., Tang Y. L., Deng Z. X., Sun M., Hong K.. ( 2013;). Micromonospora wenchangensis sp. nov., isolated from mangrove soil. Int J Syst Evol Microbiol 63: 2389–2395 [CrossRef] [PubMed].
    [Google Scholar]
  26. Rzhetsky A., Nei M.. ( 1993;). Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10: 1073–1095 [PubMed].
    [Google Scholar]
  27. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  28. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313–340 [CrossRef].
    [Google Scholar]
  29. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28: 226–231 [PubMed].
    [Google Scholar]
  30. Supong K., Suriyachadkun C., Tanasupawat S., Suwanborirux K., Pittayakhajonwut P., Kudo T., Thawai C.. ( 2013;). Micromonospora sediminicola sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 63: 570–575 [CrossRef] [PubMed].
    [Google Scholar]
  31. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24: 1596–1599 [CrossRef] [PubMed].
    [Google Scholar]
  32. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  33. Tindall B. J.. ( 1990;). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  34. Tsukamura M.. ( 1966;). Adansonian classification of mycobacteria. J Gen Microbiol 45: 253–273 [CrossRef] [PubMed].
    [Google Scholar]
  35. Wayne L., Brenner D., Colwell R., Grimont P., Kandler O., Krichevsky M., Moore L., Moore W., Murray R., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  36. Williams S. T., Goodfellow M., Alderson G., Wellington E.M.H., Sneath P.H.A., Sackin M. J.. ( 1983;). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129: 1743–1813 [PubMed].
    [Google Scholar]
  37. Xiang W., Yu C., Liu C., Zhao J., Yang L., Xie B., Li L., Hong K., Wang X.. ( 2014;). Micromonospora polyrhachis sp. nov., an actinomycete isolated from edible Chinese black ant (Polyrhachis vicina Roger). Int J Syst Evol Microbiol 64: 495–500 [CrossRef] [PubMed].
    [Google Scholar]
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