1887

Abstract

Four bacterial strains, SN-14, SN-4, M6-46 and M6-58B, were isolated from water of ponds of two salterns located in Huelva (Spain). They were Gram-stain-negative, aerobic and slightly curved rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains belong to the genus , being related most closely to F23 (98.4–98.0% sequence similarity), CL-SP19 (98.3–98.0%), TPS4-2 (97.9–97.4%), OS145 (97.5–97.4%) and KMM 231 (97.6–97.0%). The level of similarity with the type species of the genus, KMM 227, was 97.2–96.7%. The novel strains exhibited optimal growth at 5–10% (w/v) total salts, pH 7 and 37 °C. The major fatty acids of strain SN-14 were iso-C, iso-C, Cω7/Cω6, C and iso-Cω9/C 10-methyl. The DNA G+C content range was 47.6–50.8 mol%. The level of DNA–DNA relatedness between strain SN-14 and F23 was 13%, while those between strain SN-14 and the other three new isolates were between 77 and 99%. These data demonstrated that the four isolates constitute a novel species of the genus . Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the four strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SN-14 ( = CCM 8471 = CECT 8360 = LMG 27613).

Funding
This study was supported by the:
  • Spanish Ministry of Science and Innovation (Award CGL2013-46941-P)
  • Junta de Andalucía (Award P10-CVI-6226)
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2015-12-01
2024-12-14
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References

  1. Arahal D. R., García M. T., Ludwig W., Schleifer K. H., Ventosa A. ( 2001a;). Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51 14431448 [View Article] [PubMed].
    [Google Scholar]
  2. Arahal D. R., García M. T., Vargas C., Cánovas D., Nieto J. J., Ventosa A. ( 2001b;). Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174. Int J Syst Evol Microbiol 51 14571462 [View Article] [PubMed].
    [Google Scholar]
  3. Clarke P. H. ( 1953;). Hydrogen sulphide production by bacteria. J Gen Microbiol 8 397407 [View Article] [PubMed].
    [Google Scholar]
  4. Cowan S. T., Steel K. J. ( 1977). Manual for the Identification of Medical Bacteria London: Cambridge University Press;.
    [Google Scholar]
  5. De Ley J., Tijtgat R. ( 1970;). Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36 461474 [View Article] [PubMed].
    [Google Scholar]
  6. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  7. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  8. Fitch W. M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  9. Ivanova E. P., Romanenko L. A., Chun J., Matte M. H., Matte G. R., Mikhailov V. V., Svetashev V. I., Huq A., Maugel T., Colwell R. R. ( 2000;). Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50 901907 [View Article] [PubMed].
    [Google Scholar]
  10. Jean W. D., Shieh W. Y., Chiu H. H. ( 2006;). Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae . Int J Syst Evol Microbiol 56 899905 [View Article] [PubMed].
    [Google Scholar]
  11. Johnson J. L. ( 1994;). Similarity analysis of DNAs. . In Methods for General and Molecular Bacteriology, pp. 655681. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  12. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  13. Koser S. A. ( 2019;). Utilization of the salts of organic acids by the colon-aerogenes group. J Bacteriol 8 493523.
    [Google Scholar]
  14. Kovacs N. ( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178 703 [View Article] [PubMed].
    [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner,A., Lai T., Steppi S., other authors. ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32 13631371 [View Article] [PubMed].
    [Google Scholar]
  16. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [View Article].
    [Google Scholar]
  17. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109118 [View Article] [PubMed].
    [Google Scholar]
  18. Márquez M. C., Carrasco I. J., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. ( 2008;). Aquisalibacillus elongatus gen. nov., sp. nov., a moderately halophilic bacterium of the family Bacillaceae isolated from a saline lake. Int J Syst Evol Microbiol 58 19221926 [View Article] [PubMed].
    [Google Scholar]
  19. Martínez-Cánovas M. J., Béjar V., Martínez-Checa F., Páez R., Quesada E. ( 2004;). Idiomarina fontislapidosi sp. nov. and Idiomarina ramblicola sp. nov., isolated from inland hypersaline habitats in Spain. Int J Syst Evol Microbiol 54 17931797. [CrossRef]
    [Google Scholar]
  20. MIDI ( 2008). Sherlock Microbial Identification System Operating Manual, version 6.1 Newark, DE: MIDI Inc;.
    [Google Scholar]
  21. Owen R. J., Hill L. R. ( 1979;). The estimation of base compositions, base pairing and genome sizes of bacterial deoxyribonucleic acids. . In Identification Methods for Microbiologists , 2nd edn., pp. 277296. Edited by Skinner F. A., Lovelock D. W. London: Academic Press;.
    [Google Scholar]
  22. Parte A. C. ( 2014;). LPSN – list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42 (D1), D613D616 [View Article] [PubMed].
    [Google Scholar]
  23. Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1984;). Deleya halophila, a new species of moderately halophilic bacteria. Int J Syst Bacteriol 34 287292 [View Article].
    [Google Scholar]
  24. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 .
    [Google Scholar]
  25. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  26. Stackebrandt E., Goebel B. M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [View Article].
    [Google Scholar]
  27. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P.A.D., Kämpfer P., Maiden M.C.J., Nesme X., Rosselló-Mora R., Swings J., other authors. ( 2002;). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52 10431047.
    [Google Scholar]
  28. Taborda M., Antunes A., Tiago I., Veríssimo A., Nobre M. F., da Costa M. S. ( 2009;). Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina . Syst Appl Microbiol 32 371378 [View Article] [PubMed].
    [Google Scholar]
  29. Ventosa A., Quesada E., Rodríguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1982;). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128 19591968.
    [Google Scholar]
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