1887

Abstract

A Gram-stain-negative bacterium, strain TS-T30, was isolated from a saline lake (Lake Tuosu) in Qaidam basin, Qinghai province, China, and its taxonomic position was determined by using a polyphasic approach. Cells were non-spore-forming rods, non-motile, 0.8–1.4 μm wide and 1.9–4.0 μm long. Strain TS-T30 was strictly heterotrophic and aerobic. Catalase- and oxidase-positive. Growth was observed in the presence of 0.5–11.0 % (w/v) NaCl (optimum 3.0 %), and at 10–35 °C (optimum 25 °C) and pH 6.5–10.0 (optimum pH 8.5). Strain TS-T30 contained Cω7 as the only predominant fatty acid. The major respiratory quinone was Q-10. The DNA G+C content was 62 mol% ( ). Phylogenetic trees based on 16S rRNA gene sequences showed that strain TS-T30 formed a distinct lineage that was independent of other most closely related genera: (95.2–95.9 % 16S rRNA gene sequence similarities), (95.4 %), (92.8–94.9 %), (93.6–94.9 %), (94.7 %), (94.7 %), (93.9–94.7 %), (92.6–94.5 %) and (94.5 %). The major polar lipids were phosphatidylglycerol, one unidentified phospholipid and an unknown aminolipid; phosphatidylcholine was not detected. These data demonstrated that strain TS-T30 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is TS-T30 ( = CGMCC 1.12477 = NBRC 110969).

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2015-12-01
2019-09-21
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