1887

Abstract

A novel actinomycete, designated HM 537, was isolated from soil in Hamedan Province, Iran. Cell-wall hydrolysates of strain HM 537 contained -diaminopimelic acid, and whole-cell hydrolysates contained ribose, glucose, galactose, rhamnose and traces of mannose. The main phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol and an unknown phospholipid. MK-9(H), an unknown MK and MK-10(H) were the predominant menaquinones. The major fatty acids included iso-C, iso-C, iso-C G and 9(?)-methyl C. Strain HM 537 had the highest 16S rRNA gene sequence similarity to DSM 45457 (99.5 %) and DSM 45456 (99.0 %). DNA–DNA hybridization studies showed relatedness values of 13.8 ± 3.3 % with DSM 45457 and 16.3 ± 3.5 % with DSM 45456. Based on the results of phenotypic and genotypic studies, strain HM 537 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HM 537 ( = DSM 45486 = UTMC 00537 = CCUG 63021).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000607
2015-12-01
2020-01-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4544.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000607&mimeType=html&fmt=ahah

References

  1. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81: 461–466 [CrossRef] [PubMed].
    [Google Scholar]
  2. Collins M. D.. ( 1985;). Analysis of isoprenoid quinones. Methods Microbiol 18: 329–366 [CrossRef].
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 [CrossRef] [PubMed].
    [Google Scholar]
  4. Euzeby J. P.. ( 2015;).http://www.bacterio.net, List of Prokaryotic names with Standing in Nomenclature.. http://www.bacterio.net/index.html.
  5. Göker M., Cleland D., Saunders E., Lapidus A., Nolan M., Lucas S., Hammon N., Deshpande S., Cheng J.-F., other authors. ( 2011;). Complete genome sequence of Isosphaera pallida type strain (IS1BT). Stand Genomic Sci 4: 63–71 [CrossRef] [PubMed].
    [Google Scholar]
  6. Huss V.A.R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4: 184–192 [CrossRef] [PubMed].
    [Google Scholar]
  7. Kawato N., Shinobu R.. ( 1959;). On Streptomyces herbaricolor sp. nov., supplement: a single technique for microscopical observation. Mem Osaka Univ Lib Arts Educ B Nat Sci 8: 114–119.
    [Google Scholar]
  8. Kelly K. L.. ( 1964;). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;.
    [Google Scholar]
  9. Kroppenstedt R. M.. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phase. J Liq Chromatogr 5: 2359–2367 [CrossRef].
    [Google Scholar]
  10. Kroppenstedt R. M., Goodfellow M.. ( 2006;). The family Thermomonosporaceae, Actinocorallia, Actinomadura, Spirillospora, Thermomonospora. [CrossRef] In The Prokaryotes, vol. 3, , 3rd edn.., pp. 682–724. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E.. New York: Springer;.
    [Google Scholar]
  11. Labeda D.. ( 2006;). The Family Actinosynnemataceae. [CrossRef] In The Prokaryotes, vol. 3, , 3rd edn.., pp. 654–668. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E.. New York: Springer;.
    [Google Scholar]
  12. Labeda D. P., Kroppenstedt R. M.. ( 2000;). Phylogenetic analysis of Saccharothrix and related taxa: proposal for Actinosynnemataceae fam. nov. Int J Syst Evol Microbiol 50: 331–336 [CrossRef] [PubMed].
    [Google Scholar]
  13. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A.. ( 1984;). Saccharothrix: a new genus of the Actinomycetales related to Nocardiopsis. Int J Syst Bacteriol 34: 426–431 [CrossRef].
    [Google Scholar]
  14. Lechevalier M. P., Lechevalier H. A.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20: 435–443 [CrossRef].
    [Google Scholar]
  15. Lechevalier M. P., Lechevalier H. A.. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication no. 6), pp. 227–291. Edited by Dietz A., Thayer J.. Arlington, VA: Society for Industrial Microbiology;.
    [Google Scholar]
  16. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H.-P.. ( 2013;). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195: 413–418 [CrossRef] [PubMed].
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  18. Montero-Calasanz M. C., Göker M., Pötter G., Rohde M., Spröer C., Schumann P., Gorbushina A. A., Klenk H.-P.. ( 2013;). Geodermatophilus africanus sp. nov., a halotolerant actinomycete isolated from Saharan desert sand. Antonie van Leeuwenhoek 104: 207–216 [CrossRef] [PubMed].
    [Google Scholar]
  19. Nolan R. D., Cross T.. ( 1988;). Isolation and screening of actinomycetes. . In Actinomycetes in Biotechnology, pp. 1–8. Edited by Goodfellow M., Williams S. T., Mordarski M.. London: Academic Press; [CrossRef].
    [Google Scholar]
  20. Nonomura H., Ohara Y.. ( 1971;). Distribution of actinomycetes in soil. XI: Some new species of the genus Actinomadura Lechevalier et al. J Ferment Technol 49: 904–912.
    [Google Scholar]
  21. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. ( 1996;). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46: 1088–1092 [CrossRef] [PubMed].
    [Google Scholar]
  22. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids., MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  23. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36: 407–477 [PubMed].
    [Google Scholar]
  24. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313–340 [CrossRef].
    [Google Scholar]
  25. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28: 226–231 [PubMed].
    [Google Scholar]
  26. Vaas L.A.I., Sikorski J., Michael V., Göker M., Klenk H.-P.. ( 2012;). Visualization and curve-parameter estimation strategies for efficient exploration of phenotype microarray kinetics. PLoS One 7: e34846 [CrossRef] [PubMed].
    [Google Scholar]
  27. Vaas L.A.I., Sikorski J., Hofner B., Fiebig A., Buddruhs N., Klenk H.-P., Göker M.. ( 2013;). opm: an R package for analysing OmniLog(R) phenotype microarray data. Bioinformatics 29: 1823–1824 [CrossRef] [PubMed].
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P.A.D., Kandler O., Krichevsky M. I., Moore L. H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  29. Williams S. T., Goodfellow M., Alderson G.. ( 1989;). Genus Streptomyces Waksman and Henrici 1943, 339AL. . In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T., Sharpe M. E., Holt J. G.. Baltimore: Williams & Wilkins;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000607
Loading
/content/journal/ijsem/10.1099/ijsem.0.000607
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error