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Abstract

The novel actinomycete strain PWB-003, which produced fluostatins B and C antibiotics, was isolated from nearshore sediment collected from Panwa Cape, Phuket Province, Thailand. Data from the present polyphasic study indicated that strain PWB-003 represented a member of the genus . It produced single spores on substrate mycelia and contained -diaminopimelic acid in the cell-wall peptidoglycan. Whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. The predominant menaquinone was MK-10 (H). Cellular fatty acids comprised Cω9, iso-C, anteiso-C, iso-C and iso-C. On the basis of 16S rRNA gene sequence similarity analysis, the novel strain was closely related to LK2-10 (99.38 %), MC5-1 (99.16 %), FXJ6.011 (98.97 %), DSM 43904 (98.97 %), DSM 43817 (98.97 %), DSM 43912 and JCM 12357 (both 98.97 %). The G+C content of the DNA was 74.5 mol%. DNA–DNA relatedness values among strain PWB-003 and related type strains ranged from 11.3 ± 1.3 to 38.8 ± 1.1 %. On the basis of these observations, strain PWB-003 could be distinguished from its closely related type strains and is considered to represent a novel species of the genus , for which the name sp. nov. (type strain PWB-003 = JCM 30529 = PCU 341 = TISTR 2345) is proposed.

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2015-12-01
2021-04-10
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