1887

Abstract

Four brown-alga-degrading, Gram-stain-negative, aerobic, non-flagellated, gliding and rod-shaped bacteria, designated LMG 28520, LMG 28521, LMG 28522 and LMG 28523, were isolated from the gut of the abalone obtained in Japan. The four isolates had identical random amplified polymorphic DNA patterns and grew optimally at 25 °C, at pH 6.0–9.0 and in the presence of 1.0–4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences placed the isolates in the genus with and as closest neighbours. LMG 28520 and LMG 28522 showed 100 % DNA–DNA relatedness to each other, 16–17 % towards LMG 28216 and 17–20 % towards LMG 28318; they could be differentiated phenotypically from these established species. The predominant fatty acids of isolates LMG 28520 and LMG 28522 were summed feature 3 (iso-C 2-OH and/or Cω7), iso-C G and iso-C. Isolate LMG 28520 contained menaquinone-6 (MK-6) as the major respiratory quinone and phosphatidylethanolamine, two unknown aminolipids and an unknown lipid as the major polar lipids. The DNA G+C content was 34.4 mol% for LMG 28520 and 35.5 mol% for LMG 28522. On the basis of their phylogenetic and genetic distinctiveness, and differential phenotypic properties, the four isolates are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LMG 28520 ( = NBRC 111189).

Funding
This study was supported by the:
  • CREST program from the Japan Science and Technology Agency (JST)
  • Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation from the Japan Society for the Promotion of Science (JSPS)
  • Federal Public Planning Service – Science Policy, Belgium
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2015-12-01
2021-04-16
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