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Volume 65, Issue Pt_12

sp. nov., isolated from seawater Free

Abstract

A Gram-stain-negative, rod-shaped bacterium, strain SW112, was isolated from a seawater sample collected from the Indian Ocean. The strain was strictly aerobic and catalase- and oxidase-positive. Strain SW112 grew at 4–42 °C (optimum 30 °C), at pH 5.5–9.5 (optimum pH 7.5) and in the presence of 0–9.0 % (w/v) NaCl (optimum 2.0–3.0 %). The predominant cellular fatty acids were iso-C (29.7 %), iso-C3-OH (14.3 %) and summed feature 3 (comprising Cω7 and/or Cω6, 15.1 %). The major menaquinone was menaquinone-7 and the major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of strain SW112 was 39 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SW112 was related to members of the genus , showing the highest similarity with TS-T86and DSM 15883 (96.5 % and 96.4 % sequence similarity, respectively). On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that strain SW112 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SW112 ( = CGMCC 1.15180 = KCTC 33694).

  • Published Online:
Funding
This study was supported by the:
  • the program of China Ocean Mineral Resources R & D Association (Award DY125-15-R-02)
  • he National Basic Research Program of China (Award 2011CB808800)
© 2015 IUMS
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2015-12-01
2024-04-26
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References

  1. Akhwale J. K., Göker M., Rohde M., Schumann P., Klenk H.-P., Boga H. I. ( 2015;). Belliella kenyensis sp. nov., isolated from an alkaline lake. Int J Syst Evol Microbiol 65 457–462 [View Article] [PubMed] .
     [Google Scholar]
  2. Anil Kumar P., Srinivas T. N., Madhu S., Sravan R., Singh S., Naqvi S. W., Mayilraj S., Shivaji S. ( 2012;). Cecembia lonarensis gen. nov., sp. nov., a haloalkalitolerant bacterium of the family Cyclobacteriaceae, isolated from a haloalkaline lake and emended descriptions of the genera Indibacter, Nitritalea and Belliella.. Int J Syst Evol Microbiol 62 2252–2258 [View Article] [PubMed] .
     [Google Scholar]
  3. Arun A. B., Young C. C., Chen W. M., Hung M. H., Lai W. A., Chou J. H., Rekha P. D., Shen F. T., Su S. P. ( 2009;). Belliella pelovolcani sp. nov., isolated from a mud-volcano in Taiwan. Int J Syst Evol Microbiol 59 2534–2537 [View Article] [PubMed] .
     [Google Scholar]
  4. Brettar I., Christen R., Höfle M. G. ( 2004;). Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54 65–70 [View Article] [PubMed] .
     [Google Scholar]
  5. Chen S., Dong X. ( 2004;). Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water. Int J Syst Evol Microbiol 54 2257–2262 [View Article] [PubMed] .
     [Google Scholar]
  6. Collins M. D. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
     [Google Scholar]
  7. Dong X.-Z., Cai M.-Y. ( 2001). Determinative Manual for Routine Bacteriology Beijing: Scientific Press;.
     [Google Scholar]
  8. Embley T. M., Wait R. ( 1994;). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;.
     [Google Scholar]
  9. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed] .
     [Google Scholar]
  11. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. ( 1988;). Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38 358–361 [View Article].
     [Google Scholar]
  12. Lee Y.-J., Wagner I. D., Brice M. E., Kevbrin V. V., Mills G. L., Romanek C. S., Wiegel J. ( 2005;). Thermosediminibacter oceani gen. nov., sp. nov. and Thermosediminibacter litoriperuensis sp. nov., new anaerobic thermophilic bacteria isolated from Peru Margin. Extremophiles 9 375–383 [View Article] [PubMed] .
     [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159–167 [View Article].
     [Google Scholar]
  14. Miller L. T. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16 584–586 [PubMed].
     [Google Scholar]
  15. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  16. Stackebrandt E., Ebers J. ( 2006;). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33 152–155.
     [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24 1596–1599 [View Article] [PubMed] .
     [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed] .
     [Google Scholar]
  19. Tindall B. J. ( 1990;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66 199–202 [View Article].
     [Google Scholar]
  20. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60 249–266 [View Article] [PubMed] .
     [Google Scholar]
  21. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16 176–178.
     [Google Scholar]
  22. Zhong Z.-P., Liu Y., Liu H.-C., Wang F., Zhou Y.-G., Liu Z.-P. ( 2014;). Roseibium aquae sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 64 2812–2818 [View Article] [PubMed] .
     [Google Scholar]
  23. Zhong Z.-P., Liu Y., Hou T.-T., Zhou Y.-G., Liu H.-C., Liu Z. P. ( 2015;). Belliella aquatica sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 65 1622–1627 [View Article] [PubMed] .
     [Google Scholar]
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Belliella marina sp. nov., isolated from seawater
Int J Syst Evol Microbiol 65, 4353 (2015); https://doi.org/10.1099/ijsem.0.000578
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