1887

Abstract

A Gram-staining-negative and halotolerant bacterium, designated SN2, capable of biodegrading polycyclic aromatic hydrocarbons, was isolated from a tidal flat contaminated with crude oil in Korea. Cells were strictly aerobic, catalase- and oxidase-positive, motile rods, with a single polar flagellum. Growth was observed at 4–37 °C (optimum, 25–30 °C) at pH 6.0–9.0 (optimum, pH 7.0–7.5) and in the presence of 0.5–9.0 % (w/v) NaCl (optimum, 2.0 %). Only ubiquinone 8 was detected as the isoprenoid quinone, and summed feature 3 (comprising Cω7 and/or iso-C 2-OH), C, Cω7 and C were observed as the major cellular fatty acids. The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, a glycolipid, an aminolipid and three unidentified lipids. The DNA G+C content was 43.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SN2 formed a phylogenetic lineage with and within the genus , which was consistent with multilocus phylogenetic and MALDI-TOF MS analyses. Strain SN2 was most closely related to the type strains of , and , with 16S rRNA gene sequence similarities of 99.5, 99.3 and 98.4 % and DNA–DNA relatedness of 48.7 ± 6.6, 24.9 ± 7.5 and 27.9 ± 8.4 %, respectively. In conclusion, strain SN2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SN2 ( = KCTC 11700BP = JCM 17741 = KACC 18427).

Funding
This study was supported by the:
  • , Ministry of Environment (MOE), Republic of Korea
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2015-11-01
2021-03-07
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