1887

Abstract

A Gram-stain-positive bacterial strain, designated as NR2, isolated from noni ( L.) branch was investigated using a polyphasic taxonomic approach. The cells were small coccoid to ovoid, non-spore-forming and motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was a representative of a member of the genus , to which the most closely related neighbours were M-6-3 (97.90 % similarity), DSM 4810 (97.50 %), LMG 20345 (97.41 %), phenol-A (97.36 %), DSM 9573 (97.36 %) and LMG 19848 (97.32 %). The polar lipid profile of strain NR2 consisted of diphosphatidylglycerol, phosphatidylglycerol, unknown phospholipids and unknown glycolipids. The predominant respiratory quinone was MK-8, with MK-9 and MK-7 as minor components. The major fatty acids were anteiso-C and iso-C. Strain NR2 was clearly distinguishable from the type strains of related species on the basis of phylogenetic analysis, DNA–DNA hybridization, fatty acid composition data analysis and a range of physiological and comparison of biochemical characteristics. It is evident from the genotypic and phenotypic data that strain NR2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NR2 ( = DSM 29535 = CICC 10874).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31300008)
  • Fund of National Infrastructure of Microbial Resources (Award NIMR2015-4)
  • Beijing Nova Program (Award Z141105001814095)
  • Scientific and Technological Development Project of China National Research Institute of Food and Fermentation Industries (Award 2012KJFZ-BS-01)
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2015-11-01
2021-04-10
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References

  1. Chou J.H., Lin K.Y., Lin M.C., Sheu S.Y., Wei Y.H., Arun A.B., Young C.C., Chen W.M. ( 2007;). Brachybacterium phenoliresistens sp. nov., isolated from oil-contaminated coastal sand. Int J Syst Evol Microbiol 57 26742679 [CrossRef] [PubMed].
    [Google Scholar]
  2. Chou J.H., Cho N.T., Arun A.B., Young C.C., Chen W.M. ( 2008;). Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. Int J Syst Evol Microbiol 58 20512055 [CrossRef] [PubMed].
    [Google Scholar]
  3. Collins M.D., Brown J., Jones D. ( 1988;). Brachybacterium faecium gen. nov. sp. nov., a coryneform bacterium from poultry deep litter. Int J Syst Bacteriol 38 4548 [CrossRef].
    [Google Scholar]
  4. Eck R.V., Dayhoff M.O. ( 1966). Atlas of Protein Sequence and Structure., Silver Springs, Maryland: National Biomedical Research Foundation;.
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [CrossRef].
    [Google Scholar]
  6. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [CrossRef] [PubMed].
    [Google Scholar]
  7. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [CrossRef].
    [Google Scholar]
  8. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [CrossRef].
    [Google Scholar]
  9. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994). Methods for General and Molecular Bacteriology., Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  10. Gvozdyak O.R., Nogina T.M., Schumann P. ( 1992;). Taxonomic study of the genus Brachybacterium: Brachybacterium nesterenkovii sp. nov. Int J Syst Bacteriol 42 7478 [CrossRef] [PubMed].
    [Google Scholar]
  11. Heyrman J., Balcaen A., De Vos P., Schumann P., Swings J. ( 2002;). Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Evol Microbiol 52 16411646 [PubMed].
    [Google Scholar]
  12. Hoang V.A., Kim Y.J., Nguyen N.L., Yang D.C. ( 2014;). Brachybacterium ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 64 30633068 [CrossRef] [PubMed].
    [Google Scholar]
  13. Holding A.J., Collee J.G. ( 1971;). Routine biochemical tests. Methods Microbiol 6A 132. [CrossRef]
    [Google Scholar]
  14. Kimura M. ( 1983). Neutral Theory of Molecular Evolution., Cambridge: Cambridge University Press; [CrossRef].
    [Google Scholar]
  15. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161207 [CrossRef].
    [Google Scholar]
  16. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematic, pp. 115175. Edited by Stackebrandt E., Goodfellow M. Chichester, England: John. Willey and Sons Ltd;.
    [Google Scholar]
  17. Liu Y., Xie Q.Y., Shi W., Li L., An J.Y., Zhao Y.M., Hong K. ( 2014;). Brachybacterium huguangmaarense sp. nov., isolated from lake sediment. Int J Syst Evol Microbiol 64 16731678 [CrossRef] [PubMed].
    [Google Scholar]
  18. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [CrossRef].
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167 [CrossRef].
    [Google Scholar]
  20. Park S.K., Kim M.S., Jung M.J., Nam Y.D., Park E.J., Roh S.W., Bae J.W. ( 2011;). Brachybacterium squillarum sp. nov., isolated from salt-fermented seafood. Int J Syst Evol Microbiol 61 11181122 [CrossRef] [PubMed].
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  22. Schleifer K.H. ( 1985;). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18 123156 [CrossRef].
    [Google Scholar]
  23. Schleifer K.H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407477 [PubMed].
    [Google Scholar]
  24. Skerman V.B.D. ( 1967). A Guide to the Identification of the Genera of Bacteria , 2nd edn.., Baltimore: Williams & Wilkins;.
    [Google Scholar]
  25. Takeuchi M., Fang C.-X., Yokota A. ( 1995;). Taxonomic study of the genus Brachybacterium Proposal of Brachybacterium conglomeratum sp. nov., nom. rev., Brachybacterium paraconglomeratum sp. nov., and Brachybacterium rhamnosum sp. nov. Int J Syst Bacteriol 45 160168 [CrossRef].
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [CrossRef] [PubMed].
    [Google Scholar]
  27. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [CrossRef] [PubMed].
    [Google Scholar]
  28. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E. et al., ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [CrossRef].
    [Google Scholar]
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