1887

Abstract

A novel actinomycete, designated strain NEAU-GQTH1-3, was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, north-east China and characterized using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus and forms a stable clade with its closest relative JCM 13067 (98.2 % 16S rRNA gene sequence similarity). Moreover, morphological and chemotaxonomic properties of strain NEAU-GQTH1-3 also confirmed the affiliation of the isolate to the genus . The cell wall contained -diaminopimelic acid and the whole-cell sugars were glucose, galactose, madurose, mannose and ribose. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, phosphoglycolipid, an unidentified phospholipid and an unidentified glycolipid. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The predominant cellular fatty acids were iso-C and 10-methyl C. Mycolic acids were absent. The DNA G+C content was 70.4 mol%. However, the low level of DNA–DNA relatedness and some phenotypic characteristics allowed the isolate to be differentiated from its closest relative. Therefore, it is concluded that strain NEAU-GQTH1-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-GQTH1-3 ( = CGMCC 4.7199 = JCM 30346).

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2015-11-01
2019-10-21
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References

  1. Ara I., Kudo T.. ( 2007;). Sphaerosporangium gen. nov., a new member of the family Streptosporangiaceae, with descriptions of three new species as Sphaerosporangium melleum sp. nov., Sphaerosporangium rubeum sp. nov. and Sphaerosporangium cinnabarinum sp. nov., and transfer of Streptosporangium viridialbum Nonomura and Ohara 1960 to Sphaerosporangium viridialbum comb. nov. Actinomycetologica 21: 11–21 [CrossRef].
    [Google Scholar]
  2. Cao Y.R., Jiang Y., Xu L.H., Jiang C.L.. ( 2009;). Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China. Int J Syst Evol Microbiol 59: 1679–1684 [CrossRef] [PubMed].
    [Google Scholar]
  3. Collins M.D.. ( 1985;). Isoprenoid quinone analyses in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;.
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 [CrossRef] [PubMed].
    [Google Scholar]
  5. Duangmal K., Mingma R., Pathom-Aree W., Inahashi Y., Matsumoto A., Thamchaipenet A., Takahashi Y.. ( 2011;). Sphaerisporangium siamense sp. nov., an actinomycete isolated from rubber-tree rhizospheric soil. J Antibiot (Tokyo) 64: 293–296 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  8. Gao R., Liu C., Zhao J., Jia F., Yu C., Yang L., Wang X., Xiang W.. ( 2014;). Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora. Antonie van Leeuwenhoek 105: 307–315 [CrossRef] [PubMed].
    [Google Scholar]
  9. Gordon R.E., Barnett D.A., Handerhan J.E., Pang C.H.-N.. ( 1974;). Nocardia coeliaca. Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24: 54–63 [CrossRef].
    [Google Scholar]
  10. Hayakawa M., Nonomura H.. ( 1987;). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65: 501–509 [CrossRef].
    [Google Scholar]
  11. Huss V.A.R., Festl H., Schleifer K.H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4: 184–192 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kelly K.L.. ( 1964;). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors., Washington, DC: US Government Printing Office;.
    [Google Scholar]
  13. Kim S.B., Brown R., Oldfield C., Gilbert S.C., Iliarionov S., Goodfellow M.. ( 2000;). Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 50: 2031–2036 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  16. Lechevalier M.P., Lechevalier H.A.. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication no. 6), pp. 227–291. Edited by Dietz A., Thayer D. W.. Arlington, VA: Society for Industrial Microbiology;.
    [Google Scholar]
  17. Mandel M., Marmur J.. ( 1968;). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B: 195–206 [CrossRef].
    [Google Scholar]
  18. McKerrow J., Vagg S., McKinney T., Seviour E.M., Maszenan A.M., Brooks P., Seviour R.J.. ( 2000;). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol 30: 178–182 [CrossRef] [PubMed].
    [Google Scholar]
  19. Mingma R., Duangmal K., Trakulnaleamsai S., Thamchaipenet A., Matsumoto A., Takahashi Y.. ( 2014;). Sphaerisporangium rufum sp. nov., an endophytic actinomycete from roots of Oryza sativa L. Int J Syst Evol Microbiol 64: 1077–1082 [CrossRef] [PubMed].
    [Google Scholar]
  20. Minnikin D.E., Hutchinson I.G., Caldicott A.B., Goodfellow M.. ( 1980;). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188: 221–233 [CrossRef].
    [Google Scholar]
  21. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  22. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  23. Shirling E.B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313–340 [CrossRef].
    [Google Scholar]
  24. Smibert R.M., Krieg N.R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  25. Suriyachadkun C., Chunhametha S., Ngaemthao W., Tamura T., Kirtikara K., Sanglier J.J., Kitpreechavanich V.. ( 2011;). Sphaerisporangium krabiense sp. nov., isolated from soil. Int J Syst Evol Microbiol 61: 2890–2894 [CrossRef] [PubMed].
    [Google Scholar]
  26. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  27. Uchida K., Aida K.. ( 1977;). Acyl type of bacterial cell wall: its simple identification by a colorimetric method. J Gen Appl Microbiol 23: 249–260 [CrossRef].
    [Google Scholar]
  28. Wang X., Liu C., Cheng J., Zhang Y., Ma Z., Li L., Wang X., Xiang W.. ( 2015;). Sphaerisporangium corydalis sp. nov., isolated from the root of Corydalis yanhusuo L. Antonie van Leeuwenhoek 108: 133–139 [CrossRef] [PubMed].
    [Google Scholar]
  29. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  30. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16: 176–178.
    [Google Scholar]
  31. Xiang W., Liu C., Wang X., Du J., Xi L., Huang Y.. ( 2011;). Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol 61: 1165–1169 [CrossRef] [PubMed].
    [Google Scholar]
  32. Xing J., Liu C., Zhang Y., He H., Zhou Y., Li L., Zhao J., Liu S., Wang X., Xiang W.. ( 2015;). Sphaerisporangium dianthi sp. nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L. Antonie van Leeuwenhoek 107: 9–14 [CrossRef] [PubMed].
    [Google Scholar]
  33. Yokota A., Tamura T., Hasegawa T., Huang L.H.. ( 1993;). Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales. Int J Syst Bacteriol 43: 805–812 [CrossRef].
    [Google Scholar]
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