1887

Abstract

Five human clinical isolates of an unknown, strictly anaerobic, slow-growing, Gram-stain-negative, rod-shaped micro-organism were subjected to a polyphasic taxonomic study. Comparative 16S rRNA gene sequence-based phylogeny showed that the isolates grouped in a clade that included members of the genera , , and ; the type strain of was the closest relative with 91.5–91.7 % 16S rRNA gene sequence similarity. The novel strains were mainly asaccharolytic and unreactive in most conventional biochemical tests. Major metabolic end products in trypticase/glucose/yeast extract broth were acetic acid and propionic acid and the major cellular fatty acids were C and C, each of which could be used to differentiate the strains from . The DNA G+C content based on whole genome sequencing for the reference strain 22-5-S 12D6FAA was 57 mol%. Based on these data, a new genus, gen. nov., is proposed with one novel species, sp. nov., named after the exclusive and rare finding of the taxon in human samples. is the fifth genus of the 14 currently characterized in the phylum and the third one in subdivision B that includes human isolates. The type strain of is ADV70 ( = LMG 28163 = CCUG 65426).

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2015-11-01
2021-02-26
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