1887

Abstract

Five human clinical isolates of an unknown, strictly anaerobic, slow-growing, Gram-stain-negative, rod-shaped micro-organism were subjected to a polyphasic taxonomic study. Comparative 16S rRNA gene sequence-based phylogeny showed that the isolates grouped in a clade that included members of the genera , , and ; the type strain of was the closest relative with 91.5–91.7 % 16S rRNA gene sequence similarity. The novel strains were mainly asaccharolytic and unreactive in most conventional biochemical tests. Major metabolic end products in trypticase/glucose/yeast extract broth were acetic acid and propionic acid and the major cellular fatty acids were C and C, each of which could be used to differentiate the strains from . The DNA G+C content based on whole genome sequencing for the reference strain 22-5-S 12D6FAA was 57 mol%. Based on these data, a new genus, gen. nov., is proposed with one novel species, sp. nov., named after the exclusive and rare finding of the taxon in human samples. is the fifth genus of the 14 currently characterized in the phylum and the third one in subdivision B that includes human isolates. The type strain of is ADV70 ( = LMG 28163 = CCUG 65426).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000520
2015-11-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/3965.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000520&mimeType=html&fmt=ahah

References

  1. Belibasakis G. N., Oztürk V.Ö., Emingil G., Bostanci N.. ( 2013;). Synergistetes cluster A in saliva is associated with periodontitis. J Periodontal Res 48: 727–732 [PubMed].
    [Google Scholar]
  2. Carlier J.-P.. ( 1985;). Gas chromatography of fermentation products: its application in diagnosis of anaerobic bacteria. Bull Inst Pasteur 83: 57–69.
    [Google Scholar]
  3. Carlier J.-P., Marchandin H., Jumas-Bilak E., Lorin V., Henry C., Carrière C., Jean-Pierre H.. ( 2002;). Anaeroglobus geminatus gen. nov., sp. nov., a novel member of the family Veillonellaceae. Int J Syst Evol Microbiol 52: 983–986 [PubMed].
    [Google Scholar]
  4. Carlier J. P., K'ouas G., Bonne I., Lozniewski A., Mory F.. ( 2004;). Oribacterium sinus gen. nov., sp. nov., within the family ‘Lachnospiraceae’ (phylum Firmicutes). Int J Syst Evol Microbiol 54: 1611–1615 [CrossRef] [PubMed].
    [Google Scholar]
  5. Dereeper A., Guignon V., Blanc G., Audic S., Buffet S., Chevenet F., Dufayard J.-F., Guindon S., Lefort V., other authors. ( 2008;). Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 36: W465–W469 [CrossRef] [PubMed].
    [Google Scholar]
  6. Downes J., Vartoukian S. R., Dewhirst F. E., Izard J., Chen T., Yu W. H., Sutcliffe I. C., Wade W. G.. ( 2009;). Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum ‘Synergistetes’ isolated from the human oral cavity. Int J Syst Evol Microbiol 59: 972–980 [CrossRef] [PubMed].
    [Google Scholar]
  7. Ganesan A., Chaussonnerie S., Tarrade A., Dauga C., Bouchez T., Pelletier E., Le Paslier D., Sghir A.. ( 2008;). Cloacibacillus evryensis gen. nov., sp. nov., a novel asaccharolytic, mesophilic, amino-acid-degrading bacterium within the phylum ‘Synergistetes’, isolated from an anaerobic sludge digester. Int J Syst Evol Microbiol 58: 2003–2012 [CrossRef] [PubMed].
    [Google Scholar]
  8. Godon J. J., Morinière J., Moletta M., Gaillac M., Bru V., Delgènes J. P.. ( 2005;). Rarity associated with specific ecological niches in the bacterial world: the ‘Synergistes’ example. Environ Microbiol 7: 213–224 [CrossRef] [PubMed].
    [Google Scholar]
  9. Grice E. A., Kong H. H., Conlan S., Deming C. B., Davis J., Young A. C., Bouffard G. G., Blakesley R. W., Murray P. R., other authors. ( 2009;). Topographical and temporal diversity of the human skin microbiome. Science 324: 1190–1192 [CrossRef] [PubMed].
    [Google Scholar]
  10. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98.
    [Google Scholar]
  11. Holdeman L. V., Cato E. P., Moore W. E. C.. ( 1977;). Anaerobe Laboratory Manual, 4th edn.., Blacksburg: Virginia Polytechnic Institute/State University;.
    [Google Scholar]
  12. Hongoh Y., Sato T., Dolan M. F., Noda S., Ui S., Kudo T., Ohkuma M.. ( 2007;). The motility symbiont of the termite gut flagellate Caduceia versatilis is a member of the Synergistes group. Appl Environ Microbiol 73: 6270–6276 [CrossRef] [PubMed].
    [Google Scholar]
  13. Horz H. P., Citron D. M., Warren Y. A., Goldstein E. J. C., Conrads G.. ( 2006;). Synergistes group organisms of human origin. J Clin Microbiol 44: 2914–2920 [CrossRef] [PubMed].
    [Google Scholar]
  14. Jousimies-Somer H. R., Summanen P., Citron D. M., Baron E. J., Wexler H. M., Finegold S. M.. ( 2002;). Wadworth-KTL Anaerobic Bacteriology Manual, 6th edn.., Belmont, CA: Star Publishing Company;.
    [Google Scholar]
  15. Jumas-Bilak E., Marchandin H.. ( 2014;). Phylum Synergistetes. . In The Prokaryotes - Other Major Lineages of Bacteria and the Archaea, 4th edn.vol. 7, pp. 931–954. Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F.. Berlin: Springer;.
    [Google Scholar]
  16. Jumas-Bilak E., Carlier J.-P., Jean-Pierre H., Citron D., Bernard K., Damay A., Gay B., Teyssier C., Campos J., Marchandin H.. ( 2007;). Jonquetella anthropi gen. nov., sp. nov., the first member of the candidate phylum ‘Synergistetes’ isolated from man. Int J Syst Evol Microbiol 57: 2743–2748 [CrossRef] [PubMed].
    [Google Scholar]
  17. Jumas-Bilak E., Roudière L., Marchandin H.. ( 2009;). Description of ‘Synergistetes’ phyl. nov. and emended description of the phylum ‘Deferribacteres’ and of the family Syntrophomonadaceae, phylum ‘Firmicutes’. Int J Syst Evol Microbiol 59: 1028–1035 [CrossRef] [PubMed].
    [Google Scholar]
  18. Looft T., Levine U. Y., Stanton T. B.. ( 2013;). Cloacibacillus porcorum sp. nov., a mucin-degrading bacterium from the swine intestinal tract and emended description of the genus Cloacibacillus. Int J Syst Evol Microbiol 63: 1960–1966 [CrossRef] [PubMed].
    [Google Scholar]
  19. Marchandin H., Damay A., Roudière L., Teyssier C., Zorgniotti I., Dechaud H., Jean-Pierre H., Jumas-Bilak E.. ( 2010;). Phylogeny, diversity and host specialization in the phylum Synergistetes with emphasis on strains and clones of human origin. Res Microbiol 161: 91–100 [CrossRef] [PubMed].
    [Google Scholar]
  20. Soutschek E., Winter J., Schindler F., Kandler O.. ( 1984;). Acetomicrobium flavidum, gen. nov., sp. nov., a thermophilic, anaerobic bacterium from sewage sludge, forming acetate, CO2 and H2 from glucose. Syst Appl Microbiol 5: 377–390 [CrossRef].
    [Google Scholar]
  21. Vartoukian S. R., Palmer R. M., Wade W. G.. ( 2009;). Diversity and morphology of members of the phylum Synergistetes in periodontal health and disease. Appl Environ Microbiol 75: 3777–3786 [CrossRef] [PubMed].
    [Google Scholar]
  22. Vartoukian S. R., Palmer R. M., Wade W. G.. ( 2010;). Cultivation of a Synergistetes strain representing a previously uncultivated lineage. Environ Microbiol 12: 916–928 [CrossRef] [PubMed].
    [Google Scholar]
  23. Vartoukian S. R., Downes J., Palmer R. M., Wade W. G.. ( 2013;). Fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 63: 458–463 [CrossRef] [PubMed].
    [Google Scholar]
  24. Veys A., Callewaert W., Waelkens E., Van den Abbeele K.. ( 1989;). Application of gas-liquid chromatography to the routine identification of nonfermenting gram-negative bacteria in clinical specimens. J Clin Microbiol 27: 1538–1542 [PubMed].
    [Google Scholar]
  25. Yarza P., Yilmaz P., Pruesse E., Glöckner F. O., Ludwig W., Schleifer K. H., Whitman W. B., Euzéby J., Amann R., Rosselló-Móra R.. ( 2014;). Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 12: 635–645 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000520
Loading
/content/journal/ijsem/10.1099/ijsem.0.000520
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error