1887

Abstract

Two actinomycete strains, designated LIPI11-2-Ac034 and LIPI11-2-Ac042, were isolated from leaf litter collected from Cibodas Botanical Garden, West Java, Indonesia. Phylogenetic analysis based on 16S rRNA gene sequences suggested that both isolates belong to the genus . These isolates were closely related to and with similarity values of 98.2 % and 97.7 % respectively, for strain LIPI11-2-Ac034, and 99.0 % and 97.4–97.7 % respectively for strain LIPI11-2-Ac042. Both isolates grew well on ISP 7 medium with brown soluble pigment production. Spores were motile and sporangia were irregular. The isolates contained -diaminopimelic acid in cell-wall hydrolysates, and mannose, glucose and galactose in whole-cell hydrolysates. The predominant menaquinone of strain LIPI11-2-Ac034 was MK-9(H4) while that of strain LIPI11-2-Ac042 was MK-9(H6). The major cellular fatty acids were iso-C, iso-C and anteiso-C for strain LIPI11-2-Ac034, and iso-C, anteiso-C, iso-C and anteiso-C for strain LIPI11-2-Ac042. Phosphatidylethanolamine was detected as the diagnostic polar lipid. The DNA G+C contents of strains LIPI11-2-Ac034 and LIPI11-2-Ac042 were 71.5 and 70.7 mol%, respectively. Based on the differential phenotypic characteristics and the results of DNA–DNA hybridization and phylogenetic analysis, it is proposed that strains LIPI11-2-Ac034 and LIPI11-2-Ac042 represent two novel species of the genus , for which the names sp. nov. (type strain LIPI11-2-Ac034 = InaCC A459 = NBRC 110973) and sp. nov. (type strain LIPI11-2-Ac042 = InaCC A458 = NBRC 110974) are proposed.

Funding
This study was supported by the:
  • , Science and Technology Research Partnership for Sustainable Development (SATREPS)
  • , Japan Science and Technology Agency
  • , the Japan International Cooperation Agency (JICA)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000499
2015-11-01
2021-03-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/3824.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000499&mimeType=html&fmt=ahah

References

  1. Couch J. N. ( 1950;). Actinoplanes. A new genus of the Actinomycetales. J Elisha Mitchell Sci Soc 66 8792.
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [CrossRef].
    [Google Scholar]
  3. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [CrossRef] [PubMed].
    [Google Scholar]
  4. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [CrossRef].
    [Google Scholar]
  5. Goodfellow M., Stanton L. J., Simpson K. E., Minnikin D. E. ( 1990;). Numerical and chemical classification of Actinoplanes and some related actinomycetes. J Gen Microbiol 136 1936 [CrossRef].
    [Google Scholar]
  6. Hamada M., Yamamura H., Komukai C., Tamura T., Suzuki K., Hayakawa M. ( 2012;). Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium . J Antibiot (Tokyo) 65 427431 [CrossRef] [PubMed].
    [Google Scholar]
  7. Hayakawa M., Nonomura H. ( 1987;). Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65 501509 [CrossRef].
    [Google Scholar]
  8. Hayakawa M., Otoguro M., Takeuchi T., Yamazaki T., Iimura Y. ( 2000;). Application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. Antonie van Leeuwenhoek 78 171185 [CrossRef] [PubMed].
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [CrossRef] [PubMed].
    [Google Scholar]
  10. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [CrossRef] [PubMed].
    [Google Scholar]
  11. Koch C., Kroppenstedt R. M., Rainey F. A., Stackebrandt E. ( 1996;). 16S ribosomal DNA analysis of the genera Micromonospora, Actinoplanes, Catellatospora, Catenuloplanes, Couchioplanes, Dactylosporangium, and Pilimelia and emendation of the family Micromonosporaceae . Int J Syst Bacteriol 46 765768 [CrossRef] [PubMed].
    [Google Scholar]
  12. Lechevalier M. P., De Bièvre C., Lechevalier H. A. ( 1977;). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5 249260 [CrossRef].
    [Google Scholar]
  13. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [CrossRef].
    [Google Scholar]
  14. Palleroni N. J. ( 1979;). New species of the genus Actinoplanes, Actinoplanes ferrugineus . Int J Syst Bacteriol 29 5155 [CrossRef].
    [Google Scholar]
  15. Rayner R. W. ( 1970). A Mycological Colour Chart., Kew, UK: Commonwealth Mycological Institute and British Mycological Society;.
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  17. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  18. Schleifer K. H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407477 [PubMed].
    [Google Scholar]
  19. Shirling E. B., Gottlieb D. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16 313340 [CrossRef].
    [Google Scholar]
  20. Stackebrandt E., Kroppenstedt R. M. ( 1987;). Union of the genera Actinoplanes couch, Ampullariella couch, and Amorphosporangium couch in a redefined genus Actinoplanes . Syst Appl Microbiol 9 110114 [CrossRef].
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000499
Loading
/content/journal/ijsem/10.1099/ijsem.0.000499
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error