1887

Abstract

Four novel bacterial strains belonging to the genus were isolated from the surface of a weevil of the family Curculionidae (strain 10y-14), and from bark samples of hybrid poplar,  ×  (strains 6-3, 2-5 and 06C10-3-14), in Puyang, Henan Province, China. Phylogenetic analyses of the 16S rRNA gene and multilocus sequence analysis (MLSA) data showed that the four strains form a distinct cluster in the genus , indicating that they all belong to a single taxon within the genus. DNA–DNA hybridization levels between strain 10y-4 and LAM0618 and DSM 24639 were 58.31 and 53.92 %, respectively. This indicates that the four novel strains represent a species distinct from these two closely related species. The DNA G+C content of the novel strains was 42.1–42.6 %. The major fatty acids were iso-C and anteiso-C.The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid and unidentified aminophospholipids. The predominant menaquinones were MK-7 (90 %) and MK-6 (10 %). The major cell-wall amino acids were lysine, alanine, glutamic acid and glycine. On the basis of the MLSA and 16S rRNA gene sequence phylogenetic analyses, DNA–DNA reassociation values, DNA base composition, and biochemical and phenotypic characteristics, the four strains are considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 10y-14 ( = CFCC 11600 = KCTC 33522).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31200486)
  • National Infrastructure of Microbial Resources (Award NIMR-2014-7)
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2015-11-01
2021-04-10
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