1887

Abstract

There are two major centres of genetic diversification of common bean ( L.), the Mesoamerican and the Andean, and the legume is capable of establishing nitrogen-fixing symbioses with several rhizobia; seems to be the dominant species in both centres. Another genetic pool of common bean, in Peru and Ecuador, is receiving increasing attention, and studies of microsymbionts from the region can help to increase our knowledge about coevolution of this symbiosis. We have previously reported several putative new lineages from this region and here present data indicating that strains belonging to one of them, PEL4, represent a novel species. Based on 16S rRNA gene sequence phylogeny, PEL4 strains are positioned in the // clade, but show unique properties in several morphological, physiological and biochemical analyses, as well as in BOX-PCR profiles ( < 75 % similarity with related species). PEL4 strains also differed from related species based on multilocus sequence analysis of three housekeeping genes (, and ). Nucleotide identities of the three concatenated genes between PEL4 strains and related species ranged from 91.8 to 94.2 %, being highest with . DNA–DNA hybridization ( < 47 % DNA relatedness) and average nucleotide identity values of the whole genomes ( < 90.2 %) also supported the novel species status. The PEL4 strains were effective in nodulating and fixing N with common beans. The data supported the view that PEL4 strains represent a novel species, sp. nov. The type strain is CNPSo 671 ( = UMR 1450 = PIMAMPIRS I 5 = LMG 27578).

Funding
This study was supported by the:
  • CNPq (Award 02.13.08.001.00.00)
  • CAPES (Award 562008/2010-1)
  • Embrapa
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2015-09-01
2021-04-22
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