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Abstract

Two novel, Gram-stain-positive, rod-shaped, halotolerent bacteria, strains JG 03 and JG 05 were isolated from the rhizosphere of , an extreme halophyte. Comparative analyses of 16S rRNA gene sequences showed that they were closely related to members of the genus , with sequence similarities of 96.9–99.1 %. The sequence similarity of strains JG 03 and JG 05 with each other was 99.4 %. DNA–DNA hybridization of JG 03 and JG 05 with other species of the genus with validly published names showed reassociation values of 19.8 %–53.4 % and a value of 91.4 % between each other. The peptidoglycan type of both strains was A4α and MK-9 and MK-10 were the predominant menaquinones. The predominant fatty acid in JG 03 was anteiso-C and anteiso-C. However, iso-C, anteiso-C and anteiso-C were the major fatty acids in strain JG 05. The DNA G+C content of strains JG 03 and JG 05 was 70.0 and 70.1 mol%, respectively. In nutrient broth medium both strains grew at NaCl concentrations of up to 15 % (w/v). On the basis of chemotaxonomic characteristics and phylogenetic analyses, strains JG 03 and JG 05 should be affiliated to the genus . Strains JG 03 and JG 05 represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is JG 03 ( = DSM 23187 = IMCC 253).

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2015-09-01
2021-03-08
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