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Abstract

A Gram-stain-negative, vibrio-shaped, spheroplast-forming, motile, aerobic bacterium was isolated from the soil of a salt desert in Kutch, Gujarat, India. The strain, designated JC232, was oxidase- and catalase-positive. 16S rRNA gene sequence analysis indicated that strain JC232 was a member of the genus and was related most closely to AK4 (98.9 % similarity) and K92 (96.8 %). Genome relatedness based on DNA–DNA hybridization of strain JC232 with the type strains of closely related species was less than 40 %. The DNA G+C content of strain JC232 was 70 mol%. Phosphatidylglycerol, phosphatidylethanolamine, phosphotidylcholine, diphosphatidylglycerol, two unidentified amino lipids (AL1 and 2) and four unidentified lipids (UL1–4) were the polar lipids of strain JC232. Cω7/Cω6, C and Cω7/Cω6 were the major (>15 %) fatty acids of strain JC232, with minor amounts of C, C 3-OH/iso-C I, C 2-OH, C, C 3-OH and Ccycloω8c. Although strain JC232 shared the predominant ubiquinone system (Q10) with the type strains of and , it differed from the latter in polar lipid profile, NaCl growth range and other phenotypic/physiological properties. On the basis of morphological, physiological, genotypic, phylogenetic and chemotaxonomic analyses, strain JC232 is considered to represent a novel species of the genus , for which the name Caenispirillum sp. nov. is proposed. The type strain is JC232 ( = KCTC 42064 = NBRC 110150).

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2015-09-01
2019-10-17
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