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Abstract

A Gram-stain-negative, vibrio-shaped, spheroplast-forming, motile, aerobic bacterium was isolated from the soil of a salt desert in Kutch, Gujarat, India. The strain, designated JC232, was oxidase- and catalase-positive. 16S rRNA gene sequence analysis indicated that strain JC232 was a member of the genus and was related most closely to AK4 (98.9 % similarity) and K92 (96.8 %). Genome relatedness based on DNA–DNA hybridization of strain JC232 with the type strains of closely related species was less than 40 %. The DNA G+C content of strain JC232 was 70 mol%. Phosphatidylglycerol, phosphatidylethanolamine, phosphotidylcholine, diphosphatidylglycerol, two unidentified amino lipids (AL1 and 2) and four unidentified lipids (UL1–4) were the polar lipids of strain JC232. Cω7/Cω6, C and Cω7/Cω6 were the major (>15 %) fatty acids of strain JC232, with minor amounts of C, C 3-OH/iso-C I, C 2-OH, C, C 3-OH and Ccycloω8c. Although strain JC232 shared the predominant ubiquinone system (Q10) with the type strains of and , it differed from the latter in polar lipid profile, NaCl growth range and other phenotypic/physiological properties. On the basis of morphological, physiological, genotypic, phylogenetic and chemotaxonomic analyses, strain JC232 is considered to represent a novel species of the genus , for which the name Caenispirillum sp. nov. is proposed. The type strain is JC232 ( = KCTC 42064 = NBRC 110150).

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2015-09-01
2021-03-07
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References

  1. Biebl H., Pfennig N. ( 1981;). Isolation of members of the family Rhodospirillaceae . . In The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 267273. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. vol. 1 New York: Springer; [CrossRef].
    [Google Scholar]
  2. Cappuccino J.G., Sherman N. ( 1998). Microbiology: a Laboratory Manual , 5th edn.., Menlo Park, CA: Benjamin/Cummings;.
    [Google Scholar]
  3. CLSI ( 2003). Performance Standards for Antimicrobial Disk Diffusion Susceptibility Tests; Approved Standard., 8th edn, Supplement M2-A8 Wayne, PA: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  4. Gupta R., Singal R., Shankar A., Kuhad R.C., Saxena K. ( 1994;). A modified plate assay for screening phosphate solubilizing microorganisms. J Gen Appl Microbiol 40 255260 [CrossRef].
    [Google Scholar]
  5. Imhoff J.F. ( 2005;). Genus I. Rhodospirillum Molisch 1907, 24AL emend. Imhoff, Petri and Süling 1998, 796. . In Bergey's Mannual of Systematic Bacteriology, pp. 16. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. Part-C vol. 2 , 2nd edn.., New York: Springer; [CrossRef].
    [Google Scholar]
  6. Kates M. ( 1972). Techniques of Lipidology., New York: Elsevier; [CrossRef].
    [Google Scholar]
  7. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [CrossRef] [PubMed] .
    [Google Scholar]
  8. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [CrossRef] [PubMed] .
    [Google Scholar]
  9. Kimura H., Sashihara T., Matsusaki H., Sonomoto K., Ishizaki A. ( 1998;). Novel bacteriocin of Pediococcus sp. ISK-1 isolated from well-aged bed of fermented rice bran. Ann N Y Acad Sci 864 345348 [CrossRef] [PubMed] .
    [Google Scholar]
  10. Lakshmi K.V.N.S., Sasikala Ch., Takaichi S., Ramana Ch.V. ( 2011;). Phaeospirillum oryzae sp. nov., a spheroplast-forming, phototrophic alphaproteobacterium from a paddy soil. Int J Syst Evol Microbiol 61 16561661 [CrossRef] [PubMed] .
    [Google Scholar]
  11. Lakshmi K.V.N.S., Divyasree B., Ramprasad E.V.V., Sasikala Ch., Ramana Ch.V. ( 2014;). Reclassification of Rhodospirillum photometricum Molisch 1907, Rhodospirillum sulfurexigens Anil Kumar et al. 2008 and Rhodospirillum oryzae Lakshmi et al. 2013 in a new genus, Pararhodospirillum gen. nov., as Pararhodospirillum photometricum comb. nov., Pararhodospirillum sulfurexigens comb. nov. and Pararhodospirillum oryzae comb. nov., respectively, and emended description of the genus Rhodospirillum . Int J Syst Evol Microbiol 64 11541159. [CrossRef]
    [Google Scholar]
  12. Loper J.E., Schroth M.N. ( 1986;). Influence of bacterial sources of indole-3-acetic acid biosynthetic on root elongation of sugar beet. Phytopathology 76 386389 [CrossRef].
    [Google Scholar]
  13. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [CrossRef].
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39 159167 [CrossRef].
    [Google Scholar]
  15. Oren A., Duker S., Ritter S. ( 1996;). The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138 135140 [CrossRef].
    [Google Scholar]
  16. Ritika C., Suresh K., Kumar P.A. ( 2012;). Caenispirillum salinarum sp. nov., a member of the family Rhodospirillaceae isolated from a solar saltern. Int J Syst Evol Microbiol 62 16981702 [CrossRef] [PubMed] .
    [Google Scholar]
  17. Sasikala K., Ramana Ch.V., Raghuveer Rao P., Subrahmanyam M. ( 1990;). Photoproduction of hydrogen, nitrogenase and hydrogenase activities of free and immobilized whole cells of Rhodobacter sphaeroides OU 001. FEMS Microbiol Lett 72 2328 [CrossRef].
    [Google Scholar]
  18. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids., MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  19. Smibert R.M., Krieg N.R. ( 1981;). General characterization. . In Manual of Methods for General Microbiology, pp. 409443. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  20. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  21. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44 846849.
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis (mega) using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [CrossRef] [PubMed] .
    [Google Scholar]
  23. Tourova T.P., Antonov A.S. ( 1987;). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19 333355. [CrossRef]
    [Google Scholar]
  24. Tulasne R., Minck R., Kirn A., Krembel J. ( 1960;). [Delimitation of the concept of L forms of bacteria: protoplasts, spheroplasts and L forms]. Ann Inst Pasteur (Paris) 99 859 [in French]. [PubMed] .
    [Google Scholar]
  25. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [CrossRef].
    [Google Scholar]
  26. Xie C.H., Yokota A. ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49 345349 [CrossRef] [PubMed] .
    [Google Scholar]
  27. Yoon J.H., Kang S.-J., Park S., Oh T.-K. ( 2007a;). Caenispirillum bisanense gen. nov., sp. nov., isolated from sludge of a dye works. Int J Syst Evol Microbiol 57 12171221 [CrossRef] [PubMed] .
    [Google Scholar]
  28. Yoon J.H., Kang S.-J., Park S., Lee S.Y., Oh T.-K. ( 2007b;). Reclassification of Aquaspirillum itersonii and Aquaspirillum peregrinum as Novispirillum itersonii gen. nov., comb. nov. and Insolitispirillum peregrinum gen. nov., comb. nov. Int J Syst Evol Microbiol 57 28302835 [CrossRef] [PubMed] .
    [Google Scholar]
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