1887

Abstract

The taxonomic position of a strain isolated from kerosene-contaminated soil in Hungary and formerly misidentified as was examined using a polyphasic approach. The isolate, designated CHB-20p, could be clearly assigned to the genus (family ) on the basis of 16S rRNA gene sequence similarity. Strain CHB-20p, a moderate oil degrader, was a Gram-negative, aerobic, mesophilic microbe with a temperature optimum of 28–30 °C. Predominant fatty acids were iso-C, summed feature 3 (comprising C 7 and/or iso-C 2-OH) and iso-C 3-OH. Menaquinone-6 (MK-6) was the predominant respiratory quinone; MK-5 was present as a minor component. The almost complete 16S rRNA gene sequence of strain CHB-20p shared 94–97 % similarity with sequences of the type strains of species of the genus . DNA–DNA relatedness between strain CHB-20p and its closest relative, , was lower than 46 %. Moreover, several diagnostic phenotypic properties distinguished strain CHB-20p from . On the basis of biochemical, chemotaxonomic and genotypic data, isolate CHB-20p represents a novel species within the genus , sp. nov.; the type strain is CHB-20p (=NCAIM B2269=DSM 19684).

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2008-12-01
2020-01-23
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API ZYM profiles of species of the genus . [ PDF] 91 KB

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Electron micrographs of negatively stained cells of sp. nov. CHB-20p , DSM 15235 , DSM 14219 and LMG 13343 . [ PDF] 531 KB

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