Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 58, Issue 12

sp. nov., isolated from a marine electroactive biofilm Free

Abstract

Seventy isolates were obtained from a marine electroactive biofilm that was generated on a cathodically polarized stainless steel electrode (Genoa, Italy). The genetic diversity was investigated by means of BOX-PCR fingerprinting and two clusters of isolates with similar BOX-PCR profiles were delineated. Whole-cell fatty acid methyl ester analysis and 16S rRNA gene sequence analysis showed that the isolates belonged to the lineage of the class . DNA–DNA hybridization experiments and a biochemical analysis demonstrated that four isolates belonged to the species . However, 66 isolates from the second BOX-PCR cluster constituted a novel species within the genus , for which the name sp. nov. is proposed. The DNA G+C content was 61.0±0.4 %. The type strain is LMG 24367 (=CCUG 55858).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65843-0
2008-12-01
2026-01-13

Metrics

Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/12/2726.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65843-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R., Macián, M. C., Garay, E. & Pujalte, M. J.(2005).Thalassobius mediterraneus gen. nov., sp. nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov. Int J Syst Evol Microbiol 55, 2371–2376.[CrossRef] [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A.(1993).Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R., Kersters, K. & Vandamme, P.(1999). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef] [Google Scholar]
  4. Edwards, U., Rogall, T., Blocker, H., Emde, M. & Bottger, E. C.(1989). Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17, 7843–7853.[CrossRef] [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  6. Faimali, M., Chelossi, E., Garaventa, F., Corrà, C., Greco, G. & Mollica, A.(2008). Evolution of oxygen reduction current and biofilm on stainless steels cathodically polarised in natural aerated seawater. Electrochim Acta 54, 148–153.[CrossRef] [Google Scholar]
  7. González, J. M., Covert, J. S., Whitman, W. B., Henriksen, J. R., Mayer, F., Scharf, B., Schmitt, R., Buchan, A., Fuhrman, J. A. & other authors(2003).Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. Int J Syst Evol Microbiol 53, 1261–1269.[CrossRef] [Google Scholar]
  8. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  9. Lee, K., Choo, Y.-J., Giovannoni, S. J. & Cho, J.-C.(2007).Ruegeria pelagia sp. nov., isolated from the Sargasso Sea, Atlantic Ocean. Int J Syst Evol Microbiol 57, 1815–1818.[CrossRef] [Google Scholar]
  10. Martens, T., Heidorn, T., Pukall, R., Simon, M., Tindall, B. J. & Brinkhoff, T.(2006). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. Int J Syst Evol Microbiol 56, 1293–1304.[CrossRef] [Google Scholar]
  11. Mast, J., Nanbru, C., Van Den Berg, T. & Meulemans, G.(2005). Ultrastructural changes of the tracheal epithelium after vaccination of day-old chickens with the La Sota strain of Newcastle disease virus. Vet Pathol 42, 559–565.[CrossRef] [Google Scholar]
  12. Mergaert, J., Verhelst, A., Cnockaert, M. C., Tan, T. L. & Swings, J.(2001). Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences. Syst Appl Microbiol 24, 98–107.[CrossRef] [Google Scholar]
  13. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  14. Muramatsu, Y., Uchino, Y., Kasai, H., Suzuki, K. & Nakagawa, Y.(2007).Ruegeria mobilis sp. nov., a member of the Alphaproteobacteria isolated in Japan and Palau. Int J Syst Evol Microbiol 57, 1304–1309.[CrossRef] [Google Scholar]
  15. Petursdottir, S. K. & Kristjansson, J. K.(1997).Silicibacter lacuscaerulensis gen. nov., sp. nov., a mesophilic moderately halophilic bacterium characteristic of the Blue Lagoon geothermal lake in Iceland. Extremophiles 1, 94–99.[CrossRef] [Google Scholar]
  16. Pitcher, D. G., Saunders, N. A. & Owen, R. J.(1989). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef] [Google Scholar]
  17. Rademaker, J. L. W. & De Bruijn, F. J.(1997). Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer-assisted pattern analysis. In DNA Markers: Protocols, Applications, and Overviews, pp. 151–171. Edited by G. Caetano-Anollès & P. M. Gresshoff. New York: Wiley.
  18. Rademaker, J. L. W., Hoste, B., Louws, F. J., Kersters, K., Swings, J., Vauterin, L., Vauterin, P. & De Bruijn, F. J.(2000). Comparison of AFLP and rep-PCR genomic fingerprinting with DNA–DNA homology studies: Xanthomonas as a model system. Int J Syst Evol Microbiol 50, 665–677.[CrossRef] [Google Scholar]
  19. Raes, J. & Van De Peer, Y.(1999). ForCon: a software tool for the conversion of sequence alignments. (http://www.ebi.ac.uk/embnet.news/vol6_1/ForCon/body_forcon.html).
  20. Reichenbach, H. & Dworkin, M.(1981). Introduction to the gliding bacteria. In The Prokaryotes, vol. 1, pp. 315–327. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  21. Rüger, H. J. & Höfle, M. G.(1992). Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int J Syst Bacteriol 42, 133–143.[CrossRef] [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Sierra, G.(1957). A simple method for the detection of lipolytic activity of microorganisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek 23, 15–22.[CrossRef] [Google Scholar]
  24. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155. [Google Scholar]
  25. Tan, T. L. & Rüger, H. J.(1999). Enrichment, isolation, and Biolog metabolic fingerprints of oligotrophic bacteria from the Antarctic Ocean. Arch Hydrobiol Spec Issues 54, 255–272. [Google Scholar]
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  27. Uchino, Y., Hirata, A., Yokota, A. & Sugiyama, J.(1998). Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44, 201–210.[CrossRef] [Google Scholar]
  28. Vandecandelaere, I., Nercessian, O., Segaert, E., Achouak, W., Mollica, A., Faimali, M., De Vos, P. & Vandamme, P.(2008).Alteromonas genovensis sp. nov., isolated from a marine electroactive biofilm and emended description of Alteromonas macleodii Baumann et al. 1972 (Approved Lists 1980). Int J Syst Evol Microbiol 58, 2589–2596.[CrossRef] [Google Scholar]
  29. Van De Peer, Y. & De Wachter, R.(1994).treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570. [Google Scholar]
  30. Versalovic, J., Schneider, M., de Bruijn, F. J. & Lupski, J. R.(1994). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40. [Google Scholar]
  31. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  32. Yi, H., Lim, Y. W. & Chun, J.(2007). Taxonomic evaluation of the genera Ruegeria and Silicibacter: a proposal to transfer the genus Silicibacter Petursdottir and Kristjansson 1999 to the genus Ruegeria Uchino et al. 1999. Int J Syst Evol Microbiol 57, 815–819.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.65843-0
Loading
Ruegeria scottomollicae sp. nov., isolated from a marine electroactive biofilm
Int J Syst Evol Microbiol 58, 2726 (2008); https://doi.org/10.1099/ijs.0.65843-0
/content/journal/ijsem/10.1099/ijs.0.65843-0
/content/journal/ijsem/10.1099/ijs.0.65843-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2726 - 2733

Dendrogram representing the BOX-PCR profiles of the 70 isolates described in this paper. [PDF](455 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error