A strictly aerobic, non-motile, short rod-shaped actinomycete, designated strain FR1436, was isolated from sand sediment from a beach on Dokdo Island, Republic of Korea. The major menaquinone was MK-8(H), the predominant cellular fatty acid was iso-C (40.4 %) and the G+C content of the DNA was 69.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain FR1436 formed a lineage within the genus and was closely related to SBS-12 (97.8 % sequence similarity) and CL-Z59 (97.4 %). Phenotypic characteristics and DNA–DNA relatedness data served to distinguish strain FR1436 from . On the basis of the evidence presented in this study, strain FR1436 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FR1436 (=KCTC 19309 =JCM 14815).


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  1. Baik, K. S., Park, Y.-D., Seong, C. N., Kim, E. M., Bae, K. S. & Chun, J.(2006).Glaciecola nitratireducens sp. nov. isolated from seawater. Int J Syst Evol Microbiol 56, 2185–2188.[CrossRef] [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A.(1993).Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Choi, D. H., Kim, H. M., Noh, J.-H. & Cho, B. C.(2007).Nocardioides marinus sp. nov. Int J Syst Evol Microbiol 57, 775–779.[CrossRef] [Google Scholar]
  4. Chun, J. & Goodfellow, M.(1995). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef] [Google Scholar]
  5. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J.(2000).Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913. [Google Scholar]
  6. Collins, M. D.(1985). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366. [Google Scholar]
  7. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  8. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef] [Google Scholar]
  9. Gordon, R. E. & Mihm, J. M.(1962). Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 98, 628–636. [Google Scholar]
  10. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  11. Hendrickson, D. A. & Krenz, M. M.(1991). Reagents and stains. In Manual of Clinical Microbiology, pp. 1289–1314. Edited by A. Balows, W. J. Hausler, Jr, K. L. Herrmann, H. D. Isenberg & H. J. Shadomy. Washington, DC: American Society for Microbiology.
  12. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  13. Kim, H. M., Choi, D. H., Hwang, C. Y. & Cho, B. C.(2008).Nocardioides salarius sp. nov., isolated from seawater enriched with zooplankton. Int J Syst Evol Microbiol 58, 2056–2064.[CrossRef] [Google Scholar]
  14. Lawson, P. A., Collins, M. D., Schumann, P., Tindall, B. J., Hirsch, P. & Labrenz, M.(2000). New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella lacustris sp. nov. Syst Appl Microbiol 23, 219–229.[CrossRef] [Google Scholar]
  15. Lee, S. D.(2007).Nocardioides furvisabuli sp. nov., isolated from black sand. Int J Syst Evol Microbiol 57, 35–39.[CrossRef] [Google Scholar]
  16. Lee, D. W., Hyun, C.-G. & Lee, S. D.(2007).Nocardioides marinisabuli sp. nov., a novel actinobacterium isolated from beach sand. Int J Syst Evol Microbiol 57, 2960–2963.[CrossRef] [Google Scholar]
  17. Lee, S. D., Lee, D. W. & Kim, J. S.(2008).Nocardioides hwasunensis sp. nov. Int J Syst Evol Microbiol 58, 278–281.[CrossRef] [Google Scholar]
  18. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  19. MIDI(1999).Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI, Inc.
  20. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athayle, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  21. NCCLS(2003).Performance Standards for Antimicrobial Disk Susceptibility Tests, 8th edn. Approved Standard, M2-A8. Wayne, PA: National Committee for Clinical Laboratory Standards.
  22. O'Donnell, A. G., Goodfellow, M. & Minnikin, D. E.(1982). Lipids in the classification of Nocardioides: reclassification of Arthrobacter simplex (Jensen) Lochhead in the genus Nocardioides (Prauser) emend. O'Donnell et al. as Nocardioides simplex comb. nov. Arch Microbiol 133, 323–329.[CrossRef] [Google Scholar]
  23. Park, S. C., Kim, M. S., Baik, K. S., Kim, E. M., Rhee, M. S. & Seong, C. N.(2008).Chryseobacterium aquifrigidense sp. nov., isolated from a water-cooling system. Int J Syst Evol Microbiol 58, 607–611.[CrossRef] [Google Scholar]
  24. Prauser, H.(1976).Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 58–65.[CrossRef] [Google Scholar]
  25. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  26. Seong, C. N., Kim, Y. S., Baik, K. S., Lee, S. D., Hah, Y. C., Kim, S. B. & Goodfellow, M.(1999). Mycolic acid-containing actinomycetes associated with activated sludge foam. J Microbiol 37, 66–72. [Google Scholar]
  27. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  28. Stevenson, I. L.(1967). Utilisation of aromatic hydrocarbons by Arthrobacter spp. Can J Microbiol 13, 205–212.[CrossRef] [Google Scholar]
  29. Swofford, D. L.(1998). Phylogenetic analysis using parsimony (paup). Version 4. Sunderland, MA: Sinauer Associates.
  30. Tóth, E. M., Kéki, Z., Homonnay, Z. G., Borsodi, A. K., Márialigeti, K. & Schumann, P.(2008).Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera). Int J Syst Evol Microbiol 58, 78–83.[CrossRef] [Google Scholar]
  31. Tsukamura, M.(1975). Numerical analysis of the relationship between Mycobacterium, Rhodochrous group, and Nocardia by use of hypothetical median organisms. Int J Syst Bacteriol 25, 329–355.[CrossRef] [Google Scholar]
  32. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  33. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813. [Google Scholar]
  34. Yi, H. & Chun, J.(2004a).Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 1295–1299.[CrossRef] [Google Scholar]
  35. Yi, H. & Chun, J.(2004b).Nocardioides aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 2151–2154.[CrossRef] [Google Scholar]
  36. Yoon, J.-H., Kim, I.-G., Lee, M.-H. & Oh, T.-K.(2005).Nocardioides kribbensis sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 55, 1611–1614.[CrossRef] [Google Scholar]
  37. Yoon, J.-H., Lee, C.-H. & Oh, T.-K.(2006a).Nocardioides lentus sp. nov., isolated from an alkaline soil. Int J Syst Evol Microbiol 56, 271–275.[CrossRef] [Google Scholar]
  38. Yoon, J.-H., Lee, J.-K., Jung, S.-Y., Kim, J.-A., Kim, H.-K. & Oh, T.-K.(2006b).Nocardioides kongjuensis sp. nov., an N-acylhomoserine lactone-degrading bacterium. Int J Syst Evol Microbiol 56, 1783–1787.[CrossRef] [Google Scholar]
  39. Yoon, J.-H., Kang, S.-J., Lee, C.-H. & Oh, T.-K.(2007).Nocardioides insulae sp. nov., isolated from soil. Int J Syst Evol Microbiol 57, 136–140.[CrossRef] [Google Scholar]
  40. Yoon, J.-H., Kang, S.-J., Lee, M.-H. & Oh, T.-K.(2008).Nocardioides hankookensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 58, 434–437.[CrossRef] [Google Scholar]

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vol. , part 11, pp. 2619 - 2623

Fatty acid composition of strain FR1436 after 24 h incubation at 25 °C

Results from the GP2 MicroPlate (Biolog) for strain FR1436 after 2 days incubation at 25 °C on TSA

[PDF file of Supplementary Tables S1 and S2](67 KB)

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